Articular cartilage restoration in load-bearing osteochondral defects by implantation of autologous chondrocyte-fibrin constructs: an experimental study in sheep

Ovine articular chondrocytes were isolated from cartilage biopsy and culture expanded in vitro. Approximately 30 million cells per ml of cultured chondrocytes were incorporated with autologous plasma-derived fibrin to form a three-dimensional construct. Fullthickness punch hole defects were created...

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Main Authors:	Sha'ban, Munirah, Osman Cassim, Samsudin, Chen, Hui Cheng, S. H., Sharifah Salmah, Saim, Aminuddin, Idrus, Ruszymah
Format:	Article
Language:	English
Published:	The Journal of Bone and Joint Surgery 2007
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/41854/ http://irep.iium.edu.my/41854/ http://irep.iium.edu.my/41854/1/Munirah_et_al._2007.pdf

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recordtype	eprints
spelling	iium-418542015-04-16T01:36:32Z http://irep.iium.edu.my/41854/ Articular cartilage restoration in load-bearing osteochondral defects by implantation of autologous chondrocyte-fibrin constructs: an experimental study in sheep Sha'ban, Munirah Osman Cassim, Samsudin Chen, Hui Cheng S. H., Sharifah Salmah Saim, Aminuddin Idrus, Ruszymah R Medicine (General) Ovine articular chondrocytes were isolated from cartilage biopsy and culture expanded in vitro. Approximately 30 million cells per ml of cultured chondrocytes were incorporated with autologous plasma-derived fibrin to form a three-dimensional construct. Fullthickness punch hole defects were created in the lateral and medial femoral condyles. The defects were implanted with either an autologous ‘chondrocyte-fibrin’ construct (ACFC), autologous chondrocytes (ACI) or fibrin blanks (AF) as controls. Animals were killed after 12 weeks. The gross appearance of the treated defects was inspected and photographed. The repaired tissues were studied histologically and by scanning electron microscopy analysis. All defects were assessed using the International Cartilage Repair Society (ICRS) classification. Those treated with ACFC, ACI and AF exhibited median scores which correspond to a nearly-normal appearance. On the basis of the modified O’Driscoll histological scoring scale, ACFC implantation significantly enhanced cartilage repair compared to ACI and AF. Using scanning electron microscopy, ACFC and ACI showed characteristic organisation of chondrocytes and matrices, which were relatively similar to the surrounding adjacent cartilage. Implantation of ACFC resulted in superior hyaline-like cartilage regeneration when compared with ACI. If this result is applicable to humans, a better outcome would be obtained than by using conventional ACI. The Journal of Bone and Joint Surgery 2007 Article PeerReviewed application/pdf en http://irep.iium.edu.my/41854/1/Munirah_et_al._2007.pdf Sha'ban, Munirah and Osman Cassim, Samsudin and Chen, Hui Cheng and S. H., Sharifah Salmah and Saim, Aminuddin and Idrus, Ruszymah (2007) Articular cartilage restoration in load-bearing osteochondral defects by implantation of autologous chondrocyte-fibrin constructs: an experimental study in sheep. The Journal of Bone and Joint Surgery, 89-B (8). pp. 1099-1109. ISSN 0021-9355 http://www.jbjs.org.uk/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Sha'ban, Munirah Osman Cassim, Samsudin Chen, Hui Cheng S. H., Sharifah Salmah Saim, Aminuddin Idrus, Ruszymah Articular cartilage restoration in load-bearing osteochondral defects by implantation of autologous chondrocyte-fibrin constructs: an experimental study in sheep
description	Ovine articular chondrocytes were isolated from cartilage biopsy and culture expanded in vitro. Approximately 30 million cells per ml of cultured chondrocytes were incorporated with autologous plasma-derived fibrin to form a three-dimensional construct. Fullthickness punch hole defects were created in the lateral and medial femoral condyles. The defects were implanted with either an autologous ‘chondrocyte-fibrin’ construct (ACFC), autologous chondrocytes (ACI) or fibrin blanks (AF) as controls. Animals were killed after 12 weeks. The gross appearance of the treated defects was inspected and photographed. The repaired tissues were studied histologically and by scanning electron microscopy analysis. All defects were assessed using the International Cartilage Repair Society (ICRS) classification. Those treated with ACFC, ACI and AF exhibited median scores which correspond to a nearly-normal appearance. On the basis of the modified O’Driscoll histological scoring scale, ACFC implantation significantly enhanced cartilage repair compared to ACI and AF. Using scanning electron microscopy, ACFC and ACI showed characteristic organisation of chondrocytes and matrices, which were relatively similar to the surrounding adjacent cartilage. Implantation of ACFC resulted in superior hyaline-like cartilage regeneration when compared with ACI. If this result is applicable to humans, a better outcome would be obtained than by using conventional ACI.
format	Article
author	Sha'ban, Munirah Osman Cassim, Samsudin Chen, Hui Cheng S. H., Sharifah Salmah Saim, Aminuddin Idrus, Ruszymah
author_facet	Sha'ban, Munirah Osman Cassim, Samsudin Chen, Hui Cheng S. H., Sharifah Salmah Saim, Aminuddin Idrus, Ruszymah
author_sort	Sha'ban, Munirah
title	Articular cartilage restoration in load-bearing osteochondral defects by implantation of autologous chondrocyte-fibrin constructs: an experimental study in sheep
title_short	Articular cartilage restoration in load-bearing osteochondral defects by implantation of autologous chondrocyte-fibrin constructs: an experimental study in sheep
title_full	Articular cartilage restoration in load-bearing osteochondral defects by implantation of autologous chondrocyte-fibrin constructs: an experimental study in sheep
title_fullStr	Articular cartilage restoration in load-bearing osteochondral defects by implantation of autologous chondrocyte-fibrin constructs: an experimental study in sheep
title_full_unstemmed	Articular cartilage restoration in load-bearing osteochondral defects by implantation of autologous chondrocyte-fibrin constructs: an experimental study in sheep
title_sort	articular cartilage restoration in load-bearing osteochondral defects by implantation of autologous chondrocyte-fibrin constructs: an experimental study in sheep
publisher	The Journal of Bone and Joint Surgery
publishDate	2007
url	http://irep.iium.edu.my/41854/ http://irep.iium.edu.my/41854/ http://irep.iium.edu.my/41854/1/Munirah_et_al._2007.pdf
first_indexed	2023-09-18T20:59:48Z
last_indexed	2023-09-18T20:59:48Z
_version_	1777410546959646720

Articular cartilage restoration in load-bearing osteochondral defects by implantation of autologous chondrocyte-fibrin constructs: an experimental study in sheep

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