Production of laccase by Resinicium bicolor in submerged cultures: application of the Plackett-Burman experimental design to screen major factors
—The present work was aimed towards the evaluation of effects of the physical and chemical factors on laccase production by white rot fungus, Resinicium bicolor ATCC 64897 under submerged condition in shake flask using PlackettBurman design. R.bicolor potentially detoxifies ground tire rubber (...
| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2014
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/41885/ http://irep.iium.edu.my/41885/ http://irep.iium.edu.my/41885/1/41885.pdf |
| Summary: | —The present work was aimed towards the evaluation
of effects of the physical and chemical factors on laccase
production by white rot fungus, Resinicium bicolor ATCC
64897 under submerged condition in shake flask using PlackettBurman
design. R.bicolor potentially detoxifies ground tire
rubber (GTR) and facilitates growth of Thiobacillus
ferrooxidans, a bacterium that performs devulcanization of GTR
but is sensitive towards rubber additives present in GTR. In this
experiment out of 11 factors screened, copper sulphate and
yellow flame bark powder were found to be the most significant
factors affecting the laccase production. The study indicated that
the screened factors can be optimized for enhanced laccase
production and can potentially be utilized for attaining maximum
detoxification of GTR and hence further devulcanization. |
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