DNA barcoding of Stolephorus indicus, Stolephorus commersonnii and Terapon jarbua of Parangipettai Coastal Waters

Three teleost fishes, Stolephorus indicus, Stolephorus commersonnii and Terapon jarbua occurring in Parangipettai waters, were barcoded (sequenced) for 648 bp region of the mitochondrial cytochrome oxidase subunit I gene (COI) for phylogenetic analysis. All the possible barcode sequences of these th...

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Main Authors:	Khan, S. Ajmal, Lyla, P.S., John, Akbar, Kumar, C. Prasanna, Murugan, S., Khan Chowdhury, Ahmed Jalal
Format:	Article
Language:	English
Published:	Asian Network for Scientific Information 2010
Subjects:	QL Zoology
Online Access:	http://irep.iium.edu.my/4212/ http://irep.iium.edu.my/4212/ http://irep.iium.edu.my/4212/ http://irep.iium.edu.my/4212/1/373-377.pdf

id	iium-4212
recordtype	eprints
spelling	iium-42122017-12-07T05:05:11Z http://irep.iium.edu.my/4212/ DNA barcoding of Stolephorus indicus, Stolephorus commersonnii and Terapon jarbua of Parangipettai Coastal Waters Khan, S. Ajmal Lyla, P.S. John, Akbar Kumar, C. Prasanna Murugan, S. Khan Chowdhury, Ahmed Jalal QL Zoology Three teleost fishes, Stolephorus indicus, Stolephorus commersonnii and Terapon jarbua occurring in Parangipettai waters, were barcoded (sequenced) for 648 bp region of the mitochondrial cytochrome oxidase subunit I gene (COI) for phylogenetic analysis. All the possible barcode sequences of these three fishes were also extracted via FASTA format from NCBI (National Centre for Biotechnological Information). The genetic distances within the species was small compared to the genetic distances between the species i.e., the genetic distances within S. indicus was 7.67% and the average genetic distance between S. indicus and S. commersonnii was 9.11%. The genetic distance between the families Engraulidae and Terapontidae was 26.48% which was found higher than the genetic distance calculated within the family of Engraulidae (9.11%). This clearly showed that when organisms from distantly placed families are taken, the genetic distance increases. In the present study some phylogeographical signal was apparent in the data. In the neighbor-joining tree for all 10 sequences, two major groups were apparent: clade A comprising of Terapon jarbua and clade B comprising of Stolephorus species. The grouping pattern of clade A showed some phylogeographical signals. The barcode sequence of Lates calcarifer shuffled within other sequences during phylogram constructions was unambiguously placed as an out group in the phylogram. The clades after bootstrapping corresponded well with the expectations. We conclude that precise and accurate identification of Stolephorus indicus, S.commersonnii and Terapon jarbua could be performed using the barcode sequences of the mitochondrial DNA (in the COI gene) of these fishes. Asian Network for Scientific Information 2010 Article PeerReviewed application/pdf en http://irep.iium.edu.my/4212/1/373-377.pdf Khan, S. Ajmal and Lyla, P.S. and John, Akbar and Kumar, C. Prasanna and Murugan, S. and Khan Chowdhury, Ahmed Jalal (2010) DNA barcoding of Stolephorus indicus, Stolephorus commersonnii and Terapon jarbua of Parangipettai Coastal Waters. Biotechnology, 9 (3). pp. 373-377. ISSN 1682-2978 (O), 1682-296X (P) http://scialert.net/abstract/?doi=biotech.2010.373.377 10.3923/biotech.2010.373.377
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QL Zoology
spellingShingle	QL Zoology Khan, S. Ajmal Lyla, P.S. John, Akbar Kumar, C. Prasanna Murugan, S. Khan Chowdhury, Ahmed Jalal DNA barcoding of Stolephorus indicus, Stolephorus commersonnii and Terapon jarbua of Parangipettai Coastal Waters
description	Three teleost fishes, Stolephorus indicus, Stolephorus commersonnii and Terapon jarbua occurring in Parangipettai waters, were barcoded (sequenced) for 648 bp region of the mitochondrial cytochrome oxidase subunit I gene (COI) for phylogenetic analysis. All the possible barcode sequences of these three fishes were also extracted via FASTA format from NCBI (National Centre for Biotechnological Information). The genetic distances within the species was small compared to the genetic distances between the species i.e., the genetic distances within S. indicus was 7.67% and the average genetic distance between S. indicus and S. commersonnii was 9.11%. The genetic distance between the families Engraulidae and Terapontidae was 26.48% which was found higher than the genetic distance calculated within the family of Engraulidae (9.11%). This clearly showed that when organisms from distantly placed families are taken, the genetic distance increases. In the present study some phylogeographical signal was apparent in the data. In the neighbor-joining tree for all 10 sequences, two major groups were apparent: clade A comprising of Terapon jarbua and clade B comprising of Stolephorus species. The grouping pattern of clade A showed some phylogeographical signals. The barcode sequence of Lates calcarifer shuffled within other sequences during phylogram constructions was unambiguously placed as an out group in the phylogram. The clades after bootstrapping corresponded well with the expectations. We conclude that precise and accurate identification of Stolephorus indicus, S.commersonnii and Terapon jarbua could be performed using the barcode sequences of the mitochondrial DNA (in the COI gene) of these fishes.
format	Article
author	Khan, S. Ajmal Lyla, P.S. John, Akbar Kumar, C. Prasanna Murugan, S. Khan Chowdhury, Ahmed Jalal
author_facet	Khan, S. Ajmal Lyla, P.S. John, Akbar Kumar, C. Prasanna Murugan, S. Khan Chowdhury, Ahmed Jalal
author_sort	Khan, S. Ajmal
title	DNA barcoding of Stolephorus indicus, Stolephorus commersonnii and Terapon jarbua of Parangipettai Coastal Waters
title_short	DNA barcoding of Stolephorus indicus, Stolephorus commersonnii and Terapon jarbua of Parangipettai Coastal Waters
title_full	DNA barcoding of Stolephorus indicus, Stolephorus commersonnii and Terapon jarbua of Parangipettai Coastal Waters
title_fullStr	DNA barcoding of Stolephorus indicus, Stolephorus commersonnii and Terapon jarbua of Parangipettai Coastal Waters
title_full_unstemmed	DNA barcoding of Stolephorus indicus, Stolephorus commersonnii and Terapon jarbua of Parangipettai Coastal Waters
title_sort	dna barcoding of stolephorus indicus, stolephorus commersonnii and terapon jarbua of parangipettai coastal waters
publisher	Asian Network for Scientific Information
publishDate	2010
url	http://irep.iium.edu.my/4212/ http://irep.iium.edu.my/4212/ http://irep.iium.edu.my/4212/ http://irep.iium.edu.my/4212/1/373-377.pdf
first_indexed	2023-09-18T20:12:20Z
last_indexed	2023-09-18T20:12:20Z
_version_	1777407561169895424

DNA barcoding of Stolephorus indicus, Stolephorus commersonnii and Terapon jarbua of Parangipettai Coastal Waters

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