Preliminary study on biohydrogen production by bacteria from sago waste

Sago waste which has not been fully utilized is a potential substrate for biotechnology production. In lieu of that, a study focused on utilizing the waste to produce biohydrogen as the green- technology product was conducted. This study is concerned with the hydrogen gas production by Escherichia c...

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Main Authors:	Mohamad Puad, Noor Illi, Sulaiman, Sarina, Azmi, Azlin Suhaida, Shamsudin, Yusra, Mel, Maizirwan
Format:	Conference or Workshop Item
Language:	English English
Published:	2014
Subjects:	T Technology (General)
Online Access:	http://irep.iium.edu.my/42264/ http://irep.iium.edu.my/42264/2/Pse170_vetted_formatted_final.pdf http://irep.iium.edu.my/42264/5/programme_booklet.pdf

id	iium-42264
recordtype	eprints
spelling	iium-422642018-06-18T13:39:05Z http://irep.iium.edu.my/42264/ Preliminary study on biohydrogen production by bacteria from sago waste Mohamad Puad, Noor Illi Sulaiman, Sarina Azmi, Azlin Suhaida Shamsudin, Yusra Mel, Maizirwan T Technology (General) Sago waste which has not been fully utilized is a potential substrate for biotechnology production. In lieu of that, a study focused on utilizing the waste to produce biohydrogen as the green- technology product was conducted. This study is concerned with the hydrogen gas production by Escherichia coli (E. coli) using dark fermentation by utilizing sago waste as the substrate. Acid pre-treatment process for sago waste was executed and the content of reducing sugar was analysed using Dinitrosalicylic acid (DNS) method. The glucose content after pre-treatment process was 2.150 g/L when treated at the temperature of 90C for 40 minutes. Dark fermentation process was performed for 14 hours and three factors which were inoculum size (10-30%), pH (6-8) and temperature (28-40C) were investigated. The design of experiment was done using central composite design (Design Expert v6.0.8). Maximum yield of 2.22 mol H2/ mol glucose was obtained from Run 8 at processing parameters of pH 6, temperature of 40C and 30% inoculum size. The findings of this study can be applied in production of biohydrogen at a wider range of parameters (pH, temperature, inoculum size) for further studies. 2014-10 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/42264/2/Pse170_vetted_formatted_final.pdf application/pdf en http://irep.iium.edu.my/42264/5/programme_booklet.pdf Mohamad Puad, Noor Illi and Sulaiman, Sarina and Azmi, Azlin Suhaida and Shamsudin, Yusra and Mel, Maizirwan (2014) Preliminary study on biohydrogen production by bacteria from sago waste. In: 27th Symposium of Malaysian Chemical Engineers (SOMChE 2014) in conjunction with 21st Regional Symposium on Chemical Engineering (RSCE 2014), 28th-30th Oct. 2014, Taylor's University Lakeside Campus, Subang Jaya, Selangor. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	T Technology (General)
spellingShingle	T Technology (General) Mohamad Puad, Noor Illi Sulaiman, Sarina Azmi, Azlin Suhaida Shamsudin, Yusra Mel, Maizirwan Preliminary study on biohydrogen production by bacteria from sago waste
description	Sago waste which has not been fully utilized is a potential substrate for biotechnology production. In lieu of that, a study focused on utilizing the waste to produce biohydrogen as the green- technology product was conducted. This study is concerned with the hydrogen gas production by Escherichia coli (E. coli) using dark fermentation by utilizing sago waste as the substrate. Acid pre-treatment process for sago waste was executed and the content of reducing sugar was analysed using Dinitrosalicylic acid (DNS) method. The glucose content after pre-treatment process was 2.150 g/L when treated at the temperature of 90C for 40 minutes. Dark fermentation process was performed for 14 hours and three factors which were inoculum size (10-30%), pH (6-8) and temperature (28-40C) were investigated. The design of experiment was done using central composite design (Design Expert v6.0.8). Maximum yield of 2.22 mol H2/ mol glucose was obtained from Run 8 at processing parameters of pH 6, temperature of 40C and 30% inoculum size. The findings of this study can be applied in production of biohydrogen at a wider range of parameters (pH, temperature, inoculum size) for further studies.
format	Conference or Workshop Item
author	Mohamad Puad, Noor Illi Sulaiman, Sarina Azmi, Azlin Suhaida Shamsudin, Yusra Mel, Maizirwan
author_facet	Mohamad Puad, Noor Illi Sulaiman, Sarina Azmi, Azlin Suhaida Shamsudin, Yusra Mel, Maizirwan
author_sort	Mohamad Puad, Noor Illi
title	Preliminary study on biohydrogen production by bacteria from sago waste
title_short	Preliminary study on biohydrogen production by bacteria from sago waste
title_full	Preliminary study on biohydrogen production by bacteria from sago waste
title_fullStr	Preliminary study on biohydrogen production by bacteria from sago waste
title_full_unstemmed	Preliminary study on biohydrogen production by bacteria from sago waste
title_sort	preliminary study on biohydrogen production by bacteria from sago waste
publishDate	2014
url	http://irep.iium.edu.my/42264/ http://irep.iium.edu.my/42264/2/Pse170_vetted_formatted_final.pdf http://irep.iium.edu.my/42264/5/programme_booklet.pdf
first_indexed	2023-09-18T21:00:16Z
last_indexed	2023-09-18T21:00:16Z
_version_	1777410576786391040

Preliminary study on biohydrogen production by bacteria from sago waste

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