Detection and characterization of enterocin encoding genes in enterococcus mundtii strain C4L10 from the cecum of non-broiler chicken

This study was carried out to isolate and detect the enterocin genes of the bacteriocin producing strain Enterococcus mundtii C4L10 isolated from Malaysian non-broiler chicken. Amplified bacteriocin encoding genes were extracted from the total genome of Enterococcus mundtii strain C4L10 using speci...

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Main Authors:	Alhaji Yusuf, Moshood, Tengku Abdul Hamid, Tengku Haziyamin
Format:	Article
Language:	English
Published:	IJPPS 2015
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/42293/ http://irep.iium.edu.my/42293/ http://irep.iium.edu.my/42293/1/42293.pdf

id	iium-42293
recordtype	eprints
spelling	iium-422932017-08-08T02:22:01Z http://irep.iium.edu.my/42293/ Detection and characterization of enterocin encoding genes in enterococcus mundtii strain C4L10 from the cecum of non-broiler chicken Alhaji Yusuf, Moshood Tengku Abdul Hamid, Tengku Haziyamin Q Science (General) This study was carried out to isolate and detect the enterocin genes of the bacteriocin producing strain Enterococcus mundtii C4L10 isolated from Malaysian non-broiler chicken. Amplified bacteriocin encoding genes were extracted from the total genome of Enterococcus mundtii strain C4L10 using specific sets of primers. The translated protein of the resultant enterocin gene sequence showed that the bacteriocin contain 2 sets of Enterocins; Enterocin_P and Enterocin_B. The enterocin gene was found to be located in the chromosome. The bacteriocin of C4L10 has a molecular mass of approximately 10 kDa. At the amino acid level, bacteriocin of strain C4L10 is 85% homologous to enterocin_P which is similar to class IIa bacteriocins produced by Lactoctobacillus salivarius strain. The secondary structure prediction showed that the Enterocin_P contain double glycine motifs and YGNGP instead of the normal YGNGV characteristics of class IIa bacteriocin. Conserved domain search of the encoding gene revealed the presence of Glycosyltransferases domain in the Enterocin_B. The antimicrobial spot/region of the C4L10 enerocins showed the presence of two antimicrobial regions in Enterocin_P whereas only one antimicrobial spot was detected in Enterocin_B. In this study, two enterocin genes; enterocin_P and Enterocin_B were successfully amplified and characterised from Ent. Mundtii strain C4L10. The sequences showed strong similarity with reported class IIa bacteriocin, such as having double glycine and YGNGV motifs, and potential regions with antimicrobials activities which are useful for future exploitation in rational design of antimicrobial peptides. IJPPS 2015-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/42293/1/42293.pdf Alhaji Yusuf, Moshood and Tengku Abdul Hamid, Tengku Haziyamin (2015) Detection and characterization of enterocin encoding genes in enterococcus mundtii strain C4L10 from the cecum of non-broiler chicken. International Journal of Pharmacy and Pharmaceutical Sciences, 7 (4). pp. 131-136. ISSN 0975-1491 http://innovareacademics.in/journals/index.php/ijpps/article/view/3597
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Alhaji Yusuf, Moshood Tengku Abdul Hamid, Tengku Haziyamin Detection and characterization of enterocin encoding genes in enterococcus mundtii strain C4L10 from the cecum of non-broiler chicken
description	This study was carried out to isolate and detect the enterocin genes of the bacteriocin producing strain Enterococcus mundtii C4L10 isolated from Malaysian non-broiler chicken. Amplified bacteriocin encoding genes were extracted from the total genome of Enterococcus mundtii strain C4L10 using specific sets of primers. The translated protein of the resultant enterocin gene sequence showed that the bacteriocin contain 2 sets of Enterocins; Enterocin_P and Enterocin_B. The enterocin gene was found to be located in the chromosome. The bacteriocin of C4L10 has a molecular mass of approximately 10 kDa. At the amino acid level, bacteriocin of strain C4L10 is 85% homologous to enterocin_P which is similar to class IIa bacteriocins produced by Lactoctobacillus salivarius strain. The secondary structure prediction showed that the Enterocin_P contain double glycine motifs and YGNGP instead of the normal YGNGV characteristics of class IIa bacteriocin. Conserved domain search of the encoding gene revealed the presence of Glycosyltransferases domain in the Enterocin_B. The antimicrobial spot/region of the C4L10 enerocins showed the presence of two antimicrobial regions in Enterocin_P whereas only one antimicrobial spot was detected in Enterocin_B. In this study, two enterocin genes; enterocin_P and Enterocin_B were successfully amplified and characterised from Ent. Mundtii strain C4L10. The sequences showed strong similarity with reported class IIa bacteriocin, such as having double glycine and YGNGV motifs, and potential regions with antimicrobials activities which are useful for future exploitation in rational design of antimicrobial peptides.
format	Article
author	Alhaji Yusuf, Moshood Tengku Abdul Hamid, Tengku Haziyamin
author_facet	Alhaji Yusuf, Moshood Tengku Abdul Hamid, Tengku Haziyamin
author_sort	Alhaji Yusuf, Moshood
title	Detection and characterization of enterocin encoding genes in enterococcus mundtii strain C4L10 from the cecum of non-broiler chicken
title_short	Detection and characterization of enterocin encoding genes in enterococcus mundtii strain C4L10 from the cecum of non-broiler chicken
title_full	Detection and characterization of enterocin encoding genes in enterococcus mundtii strain C4L10 from the cecum of non-broiler chicken
title_fullStr	Detection and characterization of enterocin encoding genes in enterococcus mundtii strain C4L10 from the cecum of non-broiler chicken
title_full_unstemmed	Detection and characterization of enterocin encoding genes in enterococcus mundtii strain C4L10 from the cecum of non-broiler chicken
title_sort	detection and characterization of enterocin encoding genes in enterococcus mundtii strain c4l10 from the cecum of non-broiler chicken
publisher	IJPPS
publishDate	2015
url	http://irep.iium.edu.my/42293/ http://irep.iium.edu.my/42293/ http://irep.iium.edu.my/42293/1/42293.pdf
first_indexed	2023-09-18T21:00:19Z
last_indexed	2023-09-18T21:00:19Z
_version_	1777410579280953344

Detection and characterization of enterocin encoding genes in enterococcus mundtii strain C4L10 from the cecum of non-broiler chicken

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