Optimisation of hydrolysis conditions for ethanol production from sorghum starch

The conversion of starch to sugar can be achieved by hydrolysis process. The two-step enzymatic hydrolysis of sweet sorghum was performed by commercially available α-amylase and glucoamylase. An optimisation study was carried out to optimise the factors of the hydrolysis process, namely, amount of s...

Full description

Bibliographic Details
Main Authors:	Nadir, Najiah, Mel, Maizirwan, Abdul Karim, Mohamed Ismail, Mohd Yunus, Rosli
Format:	Article
Language:	English
Published:	The Institution of Engineers 2010
Subjects:	TP155 Chemical engineering TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/4240/ http://irep.iium.edu.my/4240/ http://irep.iium.edu.my/4240/1/5._Nadir_et_al_-_Journal_IEM_Malaysia.pdf

id	iium-4240
recordtype	eprints
spelling	iium-42402011-09-22T07:32:16Z http://irep.iium.edu.my/4240/ Optimisation of hydrolysis conditions for ethanol production from sorghum starch Nadir, Najiah Mel, Maizirwan Abdul Karim, Mohamed Ismail Mohd Yunus, Rosli TP155 Chemical engineering TP248.13 Biotechnology The conversion of starch to sugar can be achieved by hydrolysis process. The two-step enzymatic hydrolysis of sweet sorghum was performed by commercially available α-amylase and glucoamylase. An optimisation study was carried out to optimise the factors of the hydrolysis process, namely, amount of substrate, liquefaction and saccharification temperature, liquefaction and saccharification time, and amount of α-amylase and glucoamylase enzymes. The screening of significant hydrolysis factors were done by using the two-level factorial design (TLFD) under the factorial design (FD). The results indicated that the liquefaction and saccharification temperature, and amount of glucoamylase enzyme were found to be the major factors for further optimisation. The major factors for hydrolysis were optimised by the central composite design (CCD) under the response surface method (RSM). The analysis of variance(ANOVA) result showed that glucoamylase enzyme (p < 0.0021) and saccharification temperature (p < 0.0181) were significant factors for hydrolysis of sorghum starch. Also, the statistical analysis showed that the optimum dextrose equivalent (69.07% (g/g)) were obtained at 90°C of liquefaction temperature, 47°C of saccharification temperature, and 0.24% (v/w) of glucoamylase enzyme. The Institution of Engineers 2010-09 Article NonPeerReviewed application/pdf en http://irep.iium.edu.my/4240/1/5._Nadir_et_al_-_Journal_IEM_Malaysia.pdf Nadir, Najiah and Mel, Maizirwan and Abdul Karim, Mohamed Ismail and Mohd Yunus, Rosli (2010) Optimisation of hydrolysis conditions for ethanol production from sorghum starch. Journal - The Institution of Engineers, Malaysia, 71 (3). pp. 26-34. ISSN 0126-513X http://www.myiem.org.my/content/iem_journal_2010-181.aspx
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP155 Chemical engineering TP248.13 Biotechnology
spellingShingle	TP155 Chemical engineering TP248.13 Biotechnology Nadir, Najiah Mel, Maizirwan Abdul Karim, Mohamed Ismail Mohd Yunus, Rosli Optimisation of hydrolysis conditions for ethanol production from sorghum starch
description	The conversion of starch to sugar can be achieved by hydrolysis process. The two-step enzymatic hydrolysis of sweet sorghum was performed by commercially available α-amylase and glucoamylase. An optimisation study was carried out to optimise the factors of the hydrolysis process, namely, amount of substrate, liquefaction and saccharification temperature, liquefaction and saccharification time, and amount of α-amylase and glucoamylase enzymes. The screening of significant hydrolysis factors were done by using the two-level factorial design (TLFD) under the factorial design (FD). The results indicated that the liquefaction and saccharification temperature, and amount of glucoamylase enzyme were found to be the major factors for further optimisation. The major factors for hydrolysis were optimised by the central composite design (CCD) under the response surface method (RSM). The analysis of variance(ANOVA) result showed that glucoamylase enzyme (p < 0.0021) and saccharification temperature (p < 0.0181) were significant factors for hydrolysis of sorghum starch. Also, the statistical analysis showed that the optimum dextrose equivalent (69.07% (g/g)) were obtained at 90°C of liquefaction temperature, 47°C of saccharification temperature, and 0.24% (v/w) of glucoamylase enzyme.
format	Article
author	Nadir, Najiah Mel, Maizirwan Abdul Karim, Mohamed Ismail Mohd Yunus, Rosli
author_facet	Nadir, Najiah Mel, Maizirwan Abdul Karim, Mohamed Ismail Mohd Yunus, Rosli
author_sort	Nadir, Najiah
title	Optimisation of hydrolysis conditions for ethanol production from sorghum starch
title_short	Optimisation of hydrolysis conditions for ethanol production from sorghum starch
title_full	Optimisation of hydrolysis conditions for ethanol production from sorghum starch
title_fullStr	Optimisation of hydrolysis conditions for ethanol production from sorghum starch
title_full_unstemmed	Optimisation of hydrolysis conditions for ethanol production from sorghum starch
title_sort	optimisation of hydrolysis conditions for ethanol production from sorghum starch
publisher	The Institution of Engineers
publishDate	2010
url	http://irep.iium.edu.my/4240/ http://irep.iium.edu.my/4240/ http://irep.iium.edu.my/4240/1/5._Nadir_et_al_-_Journal_IEM_Malaysia.pdf
first_indexed	2023-09-18T20:12:23Z
last_indexed	2023-09-18T20:12:23Z
_version_	1777407563904581632

Optimisation of hydrolysis conditions for ethanol production from sorghum starch

Similar Items