Intracellular biosynthesis of Au and Ag nanoparticles using ethanolic extract of Brassica oleracea L. and studies on their physicochemical and biological properties
In this present study, we reported broccoli (Brassica oleracea L.) as a potential candidate for the synthesis of gold and silver nanoparticles (NPs) in green chemistry method. The synthesized metal nanoparticles are evaluated their antimicrobial efficacy against different human pathogenic organisms....
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Chinese Academy of Sciences
2015
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iium-424022015-07-27T08:40:22Z http://irep.iium.edu.my/42402/ Intracellular biosynthesis of Au and Ag nanoparticles using ethanolic extract of Brassica oleracea L. and studies on their physicochemical and biological properties Kuppusamy, Palaniselvam Ichwan, Solachuddin J. A. Parine, Narasimha Reddy Yusoff, Mashitah M. Maniam, Gaanty Pragas Govindan, Natanamurugaraj QR Microbiology In this present study, we reported broccoli (Brassica oleracea L.) as a potential candidate for the synthesis of gold and silver nanoparticles (NPs) in green chemistry method. The synthesized metal nanoparticles are evaluated their antimicrobial efficacy against different human pathogenic organisms. The physico-chemical properties of gold nanoparticles were analyzed using different analytical techniques such as a UV-Vis spectrophotometer, Field Emission Scanning Electron Microscopy, energy dispersive X-ray spectroscopy, X-ray diffraction and a Fourier Transform Infrared spectrophotometer. In addition, gold and silver NP antimicrobial efficacy was checked by disc diffusion assay. UV-Vis color intensity of the nanoparticles was shown at 540 and 450. nm for gold and silver nanoparticles respectively. Higher magnification of the Field Emission Scanning Electron Microscopy image shows the variable morphology of the gold nanoparticles such as spherical, rod and triangular shapes and silver nanoparticles were seen in spherical shapes. The average spherical size of the particles was observed in 24-38. nm for gold and 30-45. nm for silver NPs. X-ray diffraction pattern confirmed the presence of gold nanoparticles and silver nanoparticles which were crystalline in nature. Additionally, the functional metabolites were identified by the Fourier Transform Infrared spectroscopy. IR spectra revealed phenols, alcohols, aldehydes (sugar moieties), vitamins and proteins are present in the broccoli extract which are accountable to synthesize the nanoparticles. The synthesized gold and silver NPs inhibited the growth of the tested bacterial and fungal pathogens at the concentration of 50. ;Cg/mL respectively. In addition, broccoli mediated gold and silver nanoparticles have shown potent antimicrobial activity against human pathogens. Chinese Academy of Sciences 2015-03-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/42402/1/Journal_of_Environmental_Sciences.pdf Kuppusamy, Palaniselvam and Ichwan, Solachuddin J. A. and Parine, Narasimha Reddy and Yusoff, Mashitah M. and Maniam, Gaanty Pragas and Govindan, Natanamurugaraj (2015) Intracellular biosynthesis of Au and Ag nanoparticles using ethanolic extract of Brassica oleracea L. and studies on their physicochemical and biological properties. Journal of Environmental Sciences, 29. pp. 151-157. ISSN 1001-0742 http://www.sciencedirect.com/science/article/pii/S1001074215000169 10.1016/j.jes.2014.06.050 |
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Digital Repository |
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Local University |
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International Islamic University Malaysia |
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Online Access |
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English |
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QR Microbiology |
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QR Microbiology Kuppusamy, Palaniselvam Ichwan, Solachuddin J. A. Parine, Narasimha Reddy Yusoff, Mashitah M. Maniam, Gaanty Pragas Govindan, Natanamurugaraj Intracellular biosynthesis of Au and Ag nanoparticles using ethanolic extract of Brassica oleracea L. and studies on their physicochemical and biological properties |
| description |
In this present study, we reported broccoli (Brassica oleracea L.) as a potential candidate for the synthesis of gold and silver nanoparticles (NPs) in green chemistry method. The synthesized metal nanoparticles are evaluated their antimicrobial efficacy against different human pathogenic organisms. The physico-chemical properties of gold nanoparticles were analyzed using different analytical techniques such as a UV-Vis spectrophotometer, Field Emission Scanning Electron Microscopy, energy dispersive X-ray spectroscopy, X-ray diffraction and a Fourier Transform Infrared spectrophotometer. In addition, gold and silver NP antimicrobial efficacy was checked by disc diffusion assay. UV-Vis color intensity of the nanoparticles was shown at 540 and 450. nm for gold and silver nanoparticles respectively. Higher magnification of the Field Emission Scanning Electron Microscopy image shows the variable morphology of the gold nanoparticles such as spherical, rod and triangular shapes and silver nanoparticles were seen in spherical shapes. The average spherical size of the particles was observed in 24-38. nm for gold and 30-45. nm for silver NPs. X-ray diffraction pattern confirmed the presence of gold nanoparticles and silver nanoparticles which were crystalline in nature. Additionally, the functional metabolites were identified by the Fourier Transform Infrared spectroscopy. IR spectra revealed phenols, alcohols, aldehydes (sugar moieties), vitamins and proteins are present in the broccoli extract which are accountable to synthesize the nanoparticles. The synthesized gold and silver NPs inhibited the growth of the tested bacterial and fungal pathogens at the concentration of 50. ;Cg/mL respectively. In addition, broccoli mediated gold and silver nanoparticles have shown potent antimicrobial activity against human pathogens. |
| format |
Article |
| author |
Kuppusamy, Palaniselvam Ichwan, Solachuddin J. A. Parine, Narasimha Reddy Yusoff, Mashitah M. Maniam, Gaanty Pragas Govindan, Natanamurugaraj |
| author_facet |
Kuppusamy, Palaniselvam Ichwan, Solachuddin J. A. Parine, Narasimha Reddy Yusoff, Mashitah M. Maniam, Gaanty Pragas Govindan, Natanamurugaraj |
| author_sort |
Kuppusamy, Palaniselvam |
| title |
Intracellular biosynthesis of Au and Ag nanoparticles using ethanolic extract of Brassica oleracea L. and studies on their physicochemical and biological properties |
| title_short |
Intracellular biosynthesis of Au and Ag nanoparticles using ethanolic extract of Brassica oleracea L. and studies on their physicochemical and biological properties |
| title_full |
Intracellular biosynthesis of Au and Ag nanoparticles using ethanolic extract of Brassica oleracea L. and studies on their physicochemical and biological properties |
| title_fullStr |
Intracellular biosynthesis of Au and Ag nanoparticles using ethanolic extract of Brassica oleracea L. and studies on their physicochemical and biological properties |
| title_full_unstemmed |
Intracellular biosynthesis of Au and Ag nanoparticles using ethanolic extract of Brassica oleracea L. and studies on their physicochemical and biological properties |
| title_sort |
intracellular biosynthesis of au and ag nanoparticles using ethanolic extract of brassica oleracea l. and studies on their physicochemical and biological properties |
| publisher |
Chinese Academy of Sciences |
| publishDate |
2015 |
| url |
http://irep.iium.edu.my/42402/ http://irep.iium.edu.my/42402/ http://irep.iium.edu.my/42402/ http://irep.iium.edu.my/42402/1/Journal_of_Environmental_Sciences.pdf |
| first_indexed |
2023-09-18T21:00:26Z |
| last_indexed |
2023-09-18T21:00:26Z |
| _version_ |
1777410587460894720 |