Identification of RS9960767 at TCF4 gene in Malaysian schizophrenia and rheumatoid arhtritis patients: a preliminary study

Objectives: Mutation of a TCF4 gene was previously reported as the cause of Schizophrenia. As documented long ago, there were inverse relationship between Schizophrenia (SZ) and Rheumatoid Arthritis (RA). Aim of this study is to detect genetic associations of a Sing...

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Main Authors:	Ab Rajab, Nur Syafawati, Salleh, Mohd Razali, Mohd Yassin, Mohd Azhar, Wan Ghazali, Wan Syamimee, A.Talib, Norlelawati, Wan Taib, Wan Rohani, Sulong, Sarina
Format:	Conference or Workshop Item
Language:	English
Published:	2015
Subjects:	RZ Other systems of medicine
Online Access:	http://irep.iium.edu.my/42403/ http://irep.iium.edu.my/42403/ http://irep.iium.edu.my/42403/1/42403.pdf

id	iium-42403
recordtype	eprints
spelling	iium-424032016-01-26T08:11:44Z http://irep.iium.edu.my/42403/ Identification of RS9960767 at TCF4 gene in Malaysian schizophrenia and rheumatoid arhtritis patients: a preliminary study Ab Rajab, Nur Syafawati Salleh, Mohd Razali Mohd Yassin, Mohd Azhar Wan Ghazali, Wan Syamimee A.Talib, Norlelawati Wan Taib, Wan Rohani Sulong, Sarina RZ Other systems of medicine Objectives: Mutation of a TCF4 gene was previously reported as the cause of Schizophrenia. As documented long ago, there were inverse relationship between Schizophrenia (SZ) and Rheumatoid Arthritis (RA). Aim of this study is to detect genetic associations of a Single Nucleotide Polymorphism (SNP), rs9960767 at TCF4 gene between both patients group with controls. Methods: A total of 3ml whole blood was obtained from 47 SZ patients, 47 RA patients and 48 controls. Genomic DNA was extracted and PCR amplification of the exon 18 of TCF4 gene was performed. The PCR amplicon was digested with AluI restriction enzyme for rapid genotyping of the SNP and followed with 3% gel electrophoresis. Single association was analyzed using online SHEsis software based on Hardy-Weinberg Equilibrium using Chi-square calculation with 95% confidence interval (CI) and P value <0.05 is considered statistically significant. Results: The incidence of the mutation allele among the patients were 3.2% in SZ and 2.1% in RA while compared to controls incidence are 3.1% (p=0.98, OR=0.978; %95 CI=0.193-4.98 between SZ and controls and p=0.67, OR=1.48; %95 CI=0.24-9.09 between RA and controls). Genotype frequencies for SZ, RA and controls for AA (wild type homozygous) are 93.6 %, 97.9% and 93.8% respectively. AC genotype frequencies (mutant heterozygous) are 6.4% for SZ, 4.3% for RA and 6.2% for controls. However, none was found for allele CC (mutant homozygous) in all groups. Conclusion: The presence of SNP rs9960767 of the TCF4 gene statistically did not show association in our SZ patients. The negative association arises when the C allele from the SNP predispose to RA and protects from having SZ. However, a bigger sample size is needed to confirm this correlation. 2015-03 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/42403/1/42403.pdf Ab Rajab, Nur Syafawati and Salleh, Mohd Razali and Mohd Yassin, Mohd Azhar and Wan Ghazali, Wan Syamimee and A.Talib, Norlelawati and Wan Taib, Wan Rohani and Sulong, Sarina (2015) Identification of RS9960767 at TCF4 gene in Malaysian schizophrenia and rheumatoid arhtritis patients: a preliminary study. In: Human Genome Meeting 2015 (HGM 2015), 14th-17th March 2015, Kuala Lumpur Convention Centre, Kuala Lumpur. (Unpublished) http://www.hugo-hgm.org/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RZ Other systems of medicine
spellingShingle	RZ Other systems of medicine Ab Rajab, Nur Syafawati Salleh, Mohd Razali Mohd Yassin, Mohd Azhar Wan Ghazali, Wan Syamimee A.Talib, Norlelawati Wan Taib, Wan Rohani Sulong, Sarina Identification of RS9960767 at TCF4 gene in Malaysian schizophrenia and rheumatoid arhtritis patients: a preliminary study
description	Objectives: Mutation of a TCF4 gene was previously reported as the cause of Schizophrenia. As documented long ago, there were inverse relationship between Schizophrenia (SZ) and Rheumatoid Arthritis (RA). Aim of this study is to detect genetic associations of a Single Nucleotide Polymorphism (SNP), rs9960767 at TCF4 gene between both patients group with controls. Methods: A total of 3ml whole blood was obtained from 47 SZ patients, 47 RA patients and 48 controls. Genomic DNA was extracted and PCR amplification of the exon 18 of TCF4 gene was performed. The PCR amplicon was digested with AluI restriction enzyme for rapid genotyping of the SNP and followed with 3% gel electrophoresis. Single association was analyzed using online SHEsis software based on Hardy-Weinberg Equilibrium using Chi-square calculation with 95% confidence interval (CI) and P value <0.05 is considered statistically significant. Results: The incidence of the mutation allele among the patients were 3.2% in SZ and 2.1% in RA while compared to controls incidence are 3.1% (p=0.98, OR=0.978; %95 CI=0.193-4.98 between SZ and controls and p=0.67, OR=1.48; %95 CI=0.24-9.09 between RA and controls). Genotype frequencies for SZ, RA and controls for AA (wild type homozygous) are 93.6 %, 97.9% and 93.8% respectively. AC genotype frequencies (mutant heterozygous) are 6.4% for SZ, 4.3% for RA and 6.2% for controls. However, none was found for allele CC (mutant homozygous) in all groups. Conclusion: The presence of SNP rs9960767 of the TCF4 gene statistically did not show association in our SZ patients. The negative association arises when the C allele from the SNP predispose to RA and protects from having SZ. However, a bigger sample size is needed to confirm this correlation.
format	Conference or Workshop Item
author	Ab Rajab, Nur Syafawati Salleh, Mohd Razali Mohd Yassin, Mohd Azhar Wan Ghazali, Wan Syamimee A.Talib, Norlelawati Wan Taib, Wan Rohani Sulong, Sarina
author_facet	Ab Rajab, Nur Syafawati Salleh, Mohd Razali Mohd Yassin, Mohd Azhar Wan Ghazali, Wan Syamimee A.Talib, Norlelawati Wan Taib, Wan Rohani Sulong, Sarina
author_sort	Ab Rajab, Nur Syafawati
title	Identification of RS9960767 at TCF4 gene in Malaysian schizophrenia and rheumatoid arhtritis patients: a preliminary study
title_short	Identification of RS9960767 at TCF4 gene in Malaysian schizophrenia and rheumatoid arhtritis patients: a preliminary study
title_full	Identification of RS9960767 at TCF4 gene in Malaysian schizophrenia and rheumatoid arhtritis patients: a preliminary study
title_fullStr	Identification of RS9960767 at TCF4 gene in Malaysian schizophrenia and rheumatoid arhtritis patients: a preliminary study
title_full_unstemmed	Identification of RS9960767 at TCF4 gene in Malaysian schizophrenia and rheumatoid arhtritis patients: a preliminary study
title_sort	identification of rs9960767 at tcf4 gene in malaysian schizophrenia and rheumatoid arhtritis patients: a preliminary study
publishDate	2015
url	http://irep.iium.edu.my/42403/ http://irep.iium.edu.my/42403/ http://irep.iium.edu.my/42403/1/42403.pdf
first_indexed	2023-09-18T21:00:27Z
last_indexed	2023-09-18T21:00:27Z
_version_	1777410587594063872

Identification of RS9960767 at TCF4 gene in Malaysian schizophrenia and rheumatoid arhtritis patients: a preliminary study

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