Optimization of sonication process for high newcastle disease virus (NDV)titer

Cell disruption focuses on obtaining a desired bioproduct within a cell, and it is the cell wall that must be disrupted to allow access to the contents of the cell. In animal cells, the plasma membrane is the only barrier separating cell contents from the environment.Sound waves from sonication, a m...

Full description

Bibliographic Details
Main Authors:	Nuhu Jaafar, Jaafar, Mel, Maizirwan, Abdul Karim, Mohamed Ismail, Ideris, Aini, Syed Hassan, Sharifah
Format:	Article
Language:	English
Published:	David Publishing 2010
Subjects:	TP155 Chemical engineering TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/4245/ http://irep.iium.edu.my/4245/1/Journal_CCE-Paper_Jaafar_%28Sonication%29.pdf

id	iium-4245
recordtype	eprints
spelling	iium-42452012-02-01T03:39:28Z http://irep.iium.edu.my/4245/ Optimization of sonication process for high newcastle disease virus (NDV)titer Nuhu Jaafar, Jaafar Mel, Maizirwan Abdul Karim, Mohamed Ismail Ideris, Aini Syed Hassan, Sharifah TP155 Chemical engineering TP248.13 Biotechnology Cell disruption focuses on obtaining a desired bioproduct within a cell, and it is the cell wall that must be disrupted to allow access to the contents of the cell. In animal cells, the plasma membrane is the only barrier separating cell contents from the environment.Sound waves from sonication, a mechanical technique for cell disintegration, have been used to disrupt as well as to aggregate cells as a step towards purification of a desired bioproduct. In the present study, an improved sonication process for the high yield of Newcastle disease virus (NDV) propagated in tissue culture was described. DF-1 cell was cultured in 25cm2 T flask. When cells were about 80 confluent, a lentogenic strain of NDV (F strain) was used to infect the cell monolayer. With evident cytopathic effect, cells were subjected to cycles of freeze-thaw before sonicating with varying combinations of amplitude, temperature and time. Cells were sonicated using a water bath Sonicator, Jac Ultrasonic 1505 JEIO TECH 4 KHz. From ANOVA analysis, a significant interaction between sonication time and amplitude was observed. This also corresponds to the highest F value observed. David Publishing 2010-04 Article NonPeerReviewed application/pdf en http://irep.iium.edu.my/4245/1/Journal_CCE-Paper_Jaafar_%28Sonication%29.pdf Nuhu Jaafar, Jaafar and Mel, Maizirwan and Abdul Karim, Mohamed Ismail and Ideris, Aini and Syed Hassan, Sharifah (2010) Optimization of sonication process for high newcastle disease virus (NDV)titer. Journal of Chemistry and Chemical Engineering, 4 (4 ). pp. 49-54. ISSN 1934-7375
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP155 Chemical engineering TP248.13 Biotechnology
spellingShingle	TP155 Chemical engineering TP248.13 Biotechnology Nuhu Jaafar, Jaafar Mel, Maizirwan Abdul Karim, Mohamed Ismail Ideris, Aini Syed Hassan, Sharifah Optimization of sonication process for high newcastle disease virus (NDV)titer
description	Cell disruption focuses on obtaining a desired bioproduct within a cell, and it is the cell wall that must be disrupted to allow access to the contents of the cell. In animal cells, the plasma membrane is the only barrier separating cell contents from the environment.Sound waves from sonication, a mechanical technique for cell disintegration, have been used to disrupt as well as to aggregate cells as a step towards purification of a desired bioproduct. In the present study, an improved sonication process for the high yield of Newcastle disease virus (NDV) propagated in tissue culture was described. DF-1 cell was cultured in 25cm2 T flask. When cells were about 80 confluent, a lentogenic strain of NDV (F strain) was used to infect the cell monolayer. With evident cytopathic effect, cells were subjected to cycles of freeze-thaw before sonicating with varying combinations of amplitude, temperature and time. Cells were sonicated using a water bath Sonicator, Jac Ultrasonic 1505 JEIO TECH 4 KHz. From ANOVA analysis, a significant interaction between sonication time and amplitude was observed. This also corresponds to the highest F value observed.
format	Article
author	Nuhu Jaafar, Jaafar Mel, Maizirwan Abdul Karim, Mohamed Ismail Ideris, Aini Syed Hassan, Sharifah
author_facet	Nuhu Jaafar, Jaafar Mel, Maizirwan Abdul Karim, Mohamed Ismail Ideris, Aini Syed Hassan, Sharifah
author_sort	Nuhu Jaafar, Jaafar
title	Optimization of sonication process for high newcastle disease virus (NDV)titer
title_short	Optimization of sonication process for high newcastle disease virus (NDV)titer
title_full	Optimization of sonication process for high newcastle disease virus (NDV)titer
title_fullStr	Optimization of sonication process for high newcastle disease virus (NDV)titer
title_full_unstemmed	Optimization of sonication process for high newcastle disease virus (NDV)titer
title_sort	optimization of sonication process for high newcastle disease virus (ndv)titer
publisher	David Publishing
publishDate	2010
url	http://irep.iium.edu.my/4245/ http://irep.iium.edu.my/4245/1/Journal_CCE-Paper_Jaafar_%28Sonication%29.pdf
first_indexed	2023-09-18T20:12:24Z
last_indexed	2023-09-18T20:12:24Z
_version_	1777407564621807616

Optimization of sonication process for high newcastle disease virus (NDV)titer

Similar Items