Comparison of the neurotoxic and myotoxic effects of two Moroccan scorpion venoms and their neutralization by experimental polyclonal antivenom

Aims: Scorpion venoms contain complex mixtures of molecules, including peptides. These peptides specifically bind to various targets, in particular ion channels. Toxins modulating Na+, K+, Ca2+ and Cl − currents were described from venoms. The Androctonus and Buthus geni of scorpions are widely dis...

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Main Authors: Oukkache, Naoual, Ahmad Rusmili, Muhamad Rusdi, Othman, Iekhsan, Ghalim, Noreddine, Chgoury, Fatima, Boussadda, Lofti, Elmdaghri, Naima, Sabatier, Jean-Marc
Format: Article
Language:English
English
Published: Life Science Journal 2015
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http://irep.iium.edu.my/42591/1/Paper_Noual_2015.pdf
http://irep.iium.edu.my/42591/4/42591_Comparison%20of%20the%20neurotoxic%20and%20myotoxic%20effects%20of%20two%20Moroccan%20scorpion%20venoms%20and%20their%20neutralization%20by%20experimental%20polyclonal%20antivenom_SCOPUS.pdf
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spelling iium-425912017-11-15T02:49:57Z http://irep.iium.edu.my/42591/ Comparison of the neurotoxic and myotoxic effects of two Moroccan scorpion venoms and their neutralization by experimental polyclonal antivenom Oukkache, Naoual Ahmad Rusmili, Muhamad Rusdi Othman, Iekhsan Ghalim, Noreddine Chgoury, Fatima Boussadda, Lofti Elmdaghri, Naima Sabatier, Jean-Marc QH Natural history RM Therapeutics. Pharmacology RS Pharmacy and materia medica Aims: Scorpion venoms contain complex mixtures of molecules, including peptides. These peptides specifically bind to various targets, in particular ion channels. Toxins modulating Na+, K+, Ca2+ and Cl − currents were described from venoms. The Androctonus and Buthus geni of scorpions are widely distributed in Morocco. Their stings can cause pain, inflammation, necrosis, muscle paralysis and death. The myotoxicity is predominantly associated with neurotoxic effects and is a cause of mortality and morbidity. In this study, pharmacological effects of venoms were investigated in vitro on neuromuscular transmission. Main methods: Effects of Androctonus mauretanicus (Am) and Buthus occitanus (Bo) venoms were investigated using the chick biventer cervicis nerve-muscle preparations. The protective activity of antivenom was also 33 investigated. The antivenom was made from serum of horse that was hyperimmunizedwith Bo and Androctonus australis hector (Aah) venoms and one venom from Middle East species (Lq). The protective activity of the antivenom was assessed on the neuromuscular system by using stimulated chick nerve muscle. The results were compared with lethal activity neutralization in mice. Key findings: Am and Bo venoms contain myotoxins and postsynaptic neurotoxins. In agreement with lethal potencies of these venoms in mice, Am venom displays greater neurotoxicity and myotoxicity. The antivenom prevented lethality caused by Am, Bo and Aah venoms. The antivenom did not prevent toxic effects caused by Am venom whereas it neutralized Bo venom. Significance: Amand Bo venoms contain distinct toxins that are responsible for myotoxicity and neurotoxicity. It would be appropriate to add Am venom to produce more efficient antivenom. Life Science Journal 2015-03 Article PeerReviewed application/pdf en http://irep.iium.edu.my/42591/1/Paper_Noual_2015.pdf application/pdf en http://irep.iium.edu.my/42591/4/42591_Comparison%20of%20the%20neurotoxic%20and%20myotoxic%20effects%20of%20two%20Moroccan%20scorpion%20venoms%20and%20their%20neutralization%20by%20experimental%20polyclonal%20antivenom_SCOPUS.pdf Oukkache, Naoual and Ahmad Rusmili, Muhamad Rusdi and Othman, Iekhsan and Ghalim, Noreddine and Chgoury, Fatima and Boussadda, Lofti and Elmdaghri, Naima and Sabatier, Jean-Marc (2015) Comparison of the neurotoxic and myotoxic effects of two Moroccan scorpion venoms and their neutralization by experimental polyclonal antivenom. Life Science Journal, 124. pp. 1-7. ISSN 1097-8135 http://www.sciencedirect.com/science/article/pii/S002432051500051X 10.1016/j.lfs.2014.12.031
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
topic QH Natural history
RM Therapeutics. Pharmacology
RS Pharmacy and materia medica
spellingShingle QH Natural history
RM Therapeutics. Pharmacology
RS Pharmacy and materia medica
Oukkache, Naoual
Ahmad Rusmili, Muhamad Rusdi
Othman, Iekhsan
Ghalim, Noreddine
Chgoury, Fatima
Boussadda, Lofti
Elmdaghri, Naima
Sabatier, Jean-Marc
Comparison of the neurotoxic and myotoxic effects of two Moroccan scorpion venoms and their neutralization by experimental polyclonal antivenom
description Aims: Scorpion venoms contain complex mixtures of molecules, including peptides. These peptides specifically bind to various targets, in particular ion channels. Toxins modulating Na+, K+, Ca2+ and Cl − currents were described from venoms. The Androctonus and Buthus geni of scorpions are widely distributed in Morocco. Their stings can cause pain, inflammation, necrosis, muscle paralysis and death. The myotoxicity is predominantly associated with neurotoxic effects and is a cause of mortality and morbidity. In this study, pharmacological effects of venoms were investigated in vitro on neuromuscular transmission. Main methods: Effects of Androctonus mauretanicus (Am) and Buthus occitanus (Bo) venoms were investigated using the chick biventer cervicis nerve-muscle preparations. The protective activity of antivenom was also 33 investigated. The antivenom was made from serum of horse that was hyperimmunizedwith Bo and Androctonus australis hector (Aah) venoms and one venom from Middle East species (Lq). The protective activity of the antivenom was assessed on the neuromuscular system by using stimulated chick nerve muscle. The results were compared with lethal activity neutralization in mice. Key findings: Am and Bo venoms contain myotoxins and postsynaptic neurotoxins. In agreement with lethal potencies of these venoms in mice, Am venom displays greater neurotoxicity and myotoxicity. The antivenom prevented lethality caused by Am, Bo and Aah venoms. The antivenom did not prevent toxic effects caused by Am venom whereas it neutralized Bo venom. Significance: Amand Bo venoms contain distinct toxins that are responsible for myotoxicity and neurotoxicity. It would be appropriate to add Am venom to produce more efficient antivenom.
format Article
author Oukkache, Naoual
Ahmad Rusmili, Muhamad Rusdi
Othman, Iekhsan
Ghalim, Noreddine
Chgoury, Fatima
Boussadda, Lofti
Elmdaghri, Naima
Sabatier, Jean-Marc
author_facet Oukkache, Naoual
Ahmad Rusmili, Muhamad Rusdi
Othman, Iekhsan
Ghalim, Noreddine
Chgoury, Fatima
Boussadda, Lofti
Elmdaghri, Naima
Sabatier, Jean-Marc
author_sort Oukkache, Naoual
title Comparison of the neurotoxic and myotoxic effects of two Moroccan scorpion venoms and their neutralization by experimental polyclonal antivenom
title_short Comparison of the neurotoxic and myotoxic effects of two Moroccan scorpion venoms and their neutralization by experimental polyclonal antivenom
title_full Comparison of the neurotoxic and myotoxic effects of two Moroccan scorpion venoms and their neutralization by experimental polyclonal antivenom
title_fullStr Comparison of the neurotoxic and myotoxic effects of two Moroccan scorpion venoms and their neutralization by experimental polyclonal antivenom
title_full_unstemmed Comparison of the neurotoxic and myotoxic effects of two Moroccan scorpion venoms and their neutralization by experimental polyclonal antivenom
title_sort comparison of the neurotoxic and myotoxic effects of two moroccan scorpion venoms and their neutralization by experimental polyclonal antivenom
publisher Life Science Journal
publishDate 2015
url http://irep.iium.edu.my/42591/
http://irep.iium.edu.my/42591/
http://irep.iium.edu.my/42591/
http://irep.iium.edu.my/42591/1/Paper_Noual_2015.pdf
http://irep.iium.edu.my/42591/4/42591_Comparison%20of%20the%20neurotoxic%20and%20myotoxic%20effects%20of%20two%20Moroccan%20scorpion%20venoms%20and%20their%20neutralization%20by%20experimental%20polyclonal%20antivenom_SCOPUS.pdf
first_indexed 2023-09-18T21:00:40Z
last_indexed 2023-09-18T21:00:40Z
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