Herbal extracts exhibit anti-diabetic activities in 3T3-L1 adipocytes model

Background/Objectives: The prevalence and suffering of diabetes and obesity has been increasing among the various communities of the world including Malaysia. The cost of treatment is also rising. Therefore, it is important to explore non pharmacological regimens that are non-invasive and with less...

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Main Authors:	Azahari, Nuraniza, Khattak, Muhammad Muzaffar Ali Khan, Bakhtiar, M. Taher, Arief Ichwan, Solachuddin Jauhari
Format:	Article
Language:	English English English
Published:	Mattioli 1885 2015
Subjects:	TX341 Nutrition. Foods and food supply
Online Access:	http://irep.iium.edu.my/42775/ http://irep.iium.edu.my/42775/ http://irep.iium.edu.my/42775/1/Editors_Decision_on_the_Article.pdf http://irep.iium.edu.my/42775/2/Herbal_Extracts_Exhibit_Anti-Diabetic_Activities_in_3T3-L1_Adipocytes_Model.doc http://irep.iium.edu.my/42775/5/42775_Herbal_extracts.pdf

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recordtype	eprints
spelling	iium-427752019-09-03T02:20:32Z http://irep.iium.edu.my/42775/ Herbal extracts exhibit anti-diabetic activities in 3T3-L1 adipocytes model Azahari, Nuraniza Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher Arief Ichwan, Solachuddin Jauhari TX341 Nutrition. Foods and food supply Background/Objectives: The prevalence and suffering of diabetes and obesity has been increasing among the various communities of the world including Malaysia. The cost of treatment is also rising. Therefore, it is important to explore non pharmacological regimens that are non-invasive and with less health risks and cost burden to the patients, healthcare professionals and nations. The objective of this study was to evaluate efficacy of the traditionally used antidiabetic herbs. Methods: Water extracts of Andrographis paniculata, Lagerstroemia speciosa and Orthosiphon stamineus (Cat whisker) were prepared and evaluated for their effects on cell proliferation, adipogenesis, glucose uptake, quantification of mRNAs for Ppary, Glut4, adiponectin and leptin using Real-Time Polymerase Chain Reaction (qRT-PCR) in 3T3-L1 preadipocytes . Results: There were significant (P<0.01, P<0.05) increase in adipogenesis activity for cells treated with insulin, A. paniculata and O. stamineus compared to control. There was significant (P<0.01, P<0.05) increase in glucose uptakes in the cells treated with Sodium Orthovanadate, L. speciosa, A. paniculata and O. stamineus compared to control. Pparγ transcriptional levels in cells treated with A. paniculata extract was similar to control. However, L. speciosa extract (P˂0.01) showed significantly lower levels of Pparγ mRNA expression compared to control. Gene expression analysis demonstrated that A. paniculata and L. speciosa extracts significantly (P˂0.01, P˂0.05) induced Glut4 mRNA transcription compared to control in 3T3-L1 adipocytes. Conclusions: The present study suggests that the extracts of A. paniculata and O. stamineus possess insulin-mimicking effects. Mattioli 1885 2015-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/42775/1/Editors_Decision_on_the_Article.pdf application/pdf en http://irep.iium.edu.my/42775/2/Herbal_Extracts_Exhibit_Anti-Diabetic_Activities_in_3T3-L1_Adipocytes_Model.doc application/pdf en http://irep.iium.edu.my/42775/5/42775_Herbal_extracts.pdf Azahari, Nuraniza and Khattak, Muhammad Muzaffar Ali Khan and Bakhtiar, M. Taher and Arief Ichwan, Solachuddin Jauhari (2015) Herbal extracts exhibit anti-diabetic activities in 3T3-L1 adipocytes model. Progress in Nutrition, 17 (4). pp. 301-310. ISSN 1129-8723 http://www.mattioli1885.com/onlinejournals/index.php/progressinnutrition/index
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	TX341 Nutrition. Foods and food supply
spellingShingle	TX341 Nutrition. Foods and food supply Azahari, Nuraniza Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher Arief Ichwan, Solachuddin Jauhari Herbal extracts exhibit anti-diabetic activities in 3T3-L1 adipocytes model
description	Background/Objectives: The prevalence and suffering of diabetes and obesity has been increasing among the various communities of the world including Malaysia. The cost of treatment is also rising. Therefore, it is important to explore non pharmacological regimens that are non-invasive and with less health risks and cost burden to the patients, healthcare professionals and nations. The objective of this study was to evaluate efficacy of the traditionally used antidiabetic herbs. Methods: Water extracts of Andrographis paniculata, Lagerstroemia speciosa and Orthosiphon stamineus (Cat whisker) were prepared and evaluated for their effects on cell proliferation, adipogenesis, glucose uptake, quantification of mRNAs for Ppary, Glut4, adiponectin and leptin using Real-Time Polymerase Chain Reaction (qRT-PCR) in 3T3-L1 preadipocytes . Results: There were significant (P<0.01, P<0.05) increase in adipogenesis activity for cells treated with insulin, A. paniculata and O. stamineus compared to control. There was significant (P<0.01, P<0.05) increase in glucose uptakes in the cells treated with Sodium Orthovanadate, L. speciosa, A. paniculata and O. stamineus compared to control. Pparγ transcriptional levels in cells treated with A. paniculata extract was similar to control. However, L. speciosa extract (P˂0.01) showed significantly lower levels of Pparγ mRNA expression compared to control. Gene expression analysis demonstrated that A. paniculata and L. speciosa extracts significantly (P˂0.01, P˂0.05) induced Glut4 mRNA transcription compared to control in 3T3-L1 adipocytes. Conclusions: The present study suggests that the extracts of A. paniculata and O. stamineus possess insulin-mimicking effects.
format	Article
author	Azahari, Nuraniza Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher Arief Ichwan, Solachuddin Jauhari
author_facet	Azahari, Nuraniza Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher Arief Ichwan, Solachuddin Jauhari
author_sort	Azahari, Nuraniza
title	Herbal extracts exhibit anti-diabetic activities in 3T3-L1 adipocytes model
title_short	Herbal extracts exhibit anti-diabetic activities in 3T3-L1 adipocytes model
title_full	Herbal extracts exhibit anti-diabetic activities in 3T3-L1 adipocytes model
title_fullStr	Herbal extracts exhibit anti-diabetic activities in 3T3-L1 adipocytes model
title_full_unstemmed	Herbal extracts exhibit anti-diabetic activities in 3T3-L1 adipocytes model
title_sort	herbal extracts exhibit anti-diabetic activities in 3t3-l1 adipocytes model
publisher	Mattioli 1885
publishDate	2015
url	http://irep.iium.edu.my/42775/ http://irep.iium.edu.my/42775/ http://irep.iium.edu.my/42775/1/Editors_Decision_on_the_Article.pdf http://irep.iium.edu.my/42775/2/Herbal_Extracts_Exhibit_Anti-Diabetic_Activities_in_3T3-L1_Adipocytes_Model.doc http://irep.iium.edu.my/42775/5/42775_Herbal_extracts.pdf
first_indexed	2023-09-18T21:00:58Z
last_indexed	2023-09-18T21:00:58Z
_version_	1777410620077899776

Herbal extracts exhibit anti-diabetic activities in 3T3-L1 adipocytes model

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