Effects of oil palm (Elaeis guineensis) fruit extracts on insulin secretion from BRIN BD11 cells

Diabetes has been one of the top ten causes of death in Malaysia. Recently, the oil palm Elaeis guineensis has been explored in several antidiabetic studies. Nevertheless, the oil palm fruit has never been investigated for its antidiabetic effect. Therefore, this study was conducted to evaluate the...

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Main Authors:	Sharif, Faez, Hamid, Muhajir, Ismail, Amin, Adam, Zainah
Format:	Article
Language:	English
Published:	Nutrition Society of Malaysia 2015
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/43309/ http://irep.iium.edu.my/43309/ http://irep.iium.edu.my/43309/1/P75-82_MohdFaez.pdf

id	iium-43309
recordtype	eprints
spelling	iium-433092017-08-08T04:02:27Z http://irep.iium.edu.my/43309/ Effects of oil palm (Elaeis guineensis) fruit extracts on insulin secretion from BRIN BD11 cells Sharif, Faez Hamid, Muhajir Ismail, Amin Adam, Zainah Q Science (General) Diabetes has been one of the top ten causes of death in Malaysia. Recently, the oil palm Elaeis guineensis has been explored in several antidiabetic studies. Nevertheless, the oil palm fruit has never been investigated for its antidiabetic effect. Therefore, this study was conducted to evaluate the antidiabetic properties of oil palm fruit which specifically focusing on its effects in stimulating insulin secretion from pancreatic ß cell. The insulinotropic activity of oil palm fruit aqueous extract (OPF) on clonal pancreatic ß cell line was investigated using BRIN BD11 cell line. The cell lines were incubated with different concentrations of OPF to evaluate the stimulatory effect of OPF toward insulin secretion from BRIN BD11 cells using the Rat Insulin ELISA Assay Kits. OPF (100-1000µg/ml) were shown to significantly induce insulin secretion by a multiple of 1.97-2.58 in the BRIN BD11 cells. The highest insulin secretion increase (2.58-fold, p<0.001) was induced by 500 µg/ml in the OPF treated group. Evaluation of the possible mechanisms involved suggested that the mechanisms of insulin secreting activity of the 500 µg/ml OPF extract may involve the K+ATP channel-dependent pathway which exerts an insulin secretion effect through depolarizing the membrane of pancreatic-ß cells. The present study has revealed the presence of insulinotropic activity in Elaeis guineensis fruit. Future work assessing its use as a source of active components may provide new opportunities for the treatment of diabetes which are safe, efficient and exert a lesser amount of side effects. Nutrition Society of Malaysia 2015-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/43309/1/P75-82_MohdFaez.pdf Sharif, Faez and Hamid, Muhajir and Ismail, Amin and Adam, Zainah (2015) Effects of oil palm (Elaeis guineensis) fruit extracts on insulin secretion from BRIN BD11 cells. Malaysian Journal of Nutrition, 21 (1). pp. 75-82. ISSN 1394-035X http://www.nutriweb.org.my/publications/mjn0021_1/P75-82%20MohdFaez.php
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Sharif, Faez Hamid, Muhajir Ismail, Amin Adam, Zainah Effects of oil palm (Elaeis guineensis) fruit extracts on insulin secretion from BRIN BD11 cells
description	Diabetes has been one of the top ten causes of death in Malaysia. Recently, the oil palm Elaeis guineensis has been explored in several antidiabetic studies. Nevertheless, the oil palm fruit has never been investigated for its antidiabetic effect. Therefore, this study was conducted to evaluate the antidiabetic properties of oil palm fruit which specifically focusing on its effects in stimulating insulin secretion from pancreatic ß cell. The insulinotropic activity of oil palm fruit aqueous extract (OPF) on clonal pancreatic ß cell line was investigated using BRIN BD11 cell line. The cell lines were incubated with different concentrations of OPF to evaluate the stimulatory effect of OPF toward insulin secretion from BRIN BD11 cells using the Rat Insulin ELISA Assay Kits. OPF (100-1000µg/ml) were shown to significantly induce insulin secretion by a multiple of 1.97-2.58 in the BRIN BD11 cells. The highest insulin secretion increase (2.58-fold, p<0.001) was induced by 500 µg/ml in the OPF treated group. Evaluation of the possible mechanisms involved suggested that the mechanisms of insulin secreting activity of the 500 µg/ml OPF extract may involve the K+ATP channel-dependent pathway which exerts an insulin secretion effect through depolarizing the membrane of pancreatic-ß cells. The present study has revealed the presence of insulinotropic activity in Elaeis guineensis fruit. Future work assessing its use as a source of active components may provide new opportunities for the treatment of diabetes which are safe, efficient and exert a lesser amount of side effects.
format	Article
author	Sharif, Faez Hamid, Muhajir Ismail, Amin Adam, Zainah
author_facet	Sharif, Faez Hamid, Muhajir Ismail, Amin Adam, Zainah
author_sort	Sharif, Faez
title	Effects of oil palm (Elaeis guineensis) fruit extracts on insulin secretion from BRIN BD11 cells
title_short	Effects of oil palm (Elaeis guineensis) fruit extracts on insulin secretion from BRIN BD11 cells
title_full	Effects of oil palm (Elaeis guineensis) fruit extracts on insulin secretion from BRIN BD11 cells
title_fullStr	Effects of oil palm (Elaeis guineensis) fruit extracts on insulin secretion from BRIN BD11 cells
title_full_unstemmed	Effects of oil palm (Elaeis guineensis) fruit extracts on insulin secretion from BRIN BD11 cells
title_sort	effects of oil palm (elaeis guineensis) fruit extracts on insulin secretion from brin bd11 cells
publisher	Nutrition Society of Malaysia
publishDate	2015
url	http://irep.iium.edu.my/43309/ http://irep.iium.edu.my/43309/ http://irep.iium.edu.my/43309/1/P75-82_MohdFaez.pdf
first_indexed	2023-09-18T21:01:42Z
last_indexed	2023-09-18T21:01:42Z
_version_	1777410667007967232

Effects of oil palm (Elaeis guineensis) fruit extracts on insulin secretion from BRIN BD11 cells

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