Rapid detection of porcine and bovine gelatin in dental prophylaxis pastes using fourir transform infrared spectroscopy

Aims: To detect the presence of porcine or bovine gelatins in dental prophylaxis pastes used in Malaysia by using Fourier Transform Infrared Spectroscopy (FTIR). Materials and Methods: Four brands of commonly used dental prophylaxis paste in Malaysia were collected and labelled A, B, C, and D. Pure...

Full description

Bibliographic Details
Main Authors:	Tajudin, Noor Ashikin, Abd Aziz, Nur Fatin, Lestari, Widya, Ardini, Yunita Dewi
Format:	Conference or Workshop Item
Language:	English
Published:	2015
Subjects:	RK Dentistry
Online Access:	http://irep.iium.edu.my/43510/ http://irep.iium.edu.my/43510/1/43510.pdf

id	iium-43510
recordtype	eprints
spelling	iium-435102018-01-03T06:42:22Z http://irep.iium.edu.my/43510/ Rapid detection of porcine and bovine gelatin in dental prophylaxis pastes using fourir transform infrared spectroscopy Tajudin, Noor Ashikin Abd Aziz, Nur Fatin Lestari, Widya Ardini, Yunita Dewi RK Dentistry Aims: To detect the presence of porcine or bovine gelatins in dental prophylaxis pastes used in Malaysia by using Fourier Transform Infrared Spectroscopy (FTIR). Materials and Methods: Four brands of commonly used dental prophylaxis paste in Malaysia were collected and labelled A, B, C, and D. Pure porcine and bovine skin gelatins were used as controls. Gelatin extraction was carried out using acetone precipitation method. The extracts were then placed in the FTIR Perkin Elmer spectrometer for less than 5 minutes. All spectra were recorded within 4000– 400 cm-1 wavenumber with 4 cm-1 resolution under 40 scans. The results were analyzed by comparing the samples’ spectra with the controls’. The data were subjected to discriminant analysis using The SpectrumTM Software. Results: Sample A and porcine gelatin spectra had similar peak at 2163 cm-1 while sample B showed similarity to bovine gelatin at the range of 4000 – 2000 cm-1. Detailed analysis of the spectrum patterns of sample C in the area between 1600-1400 cm-1 and 3600-2800 cm-1 indicated an appearance of similar peaks to porcine gelatin. On the other hand, the spectrum of sample D at the range of 1800-1200 cm-1 is similar to that of bovine gelatin. Conclusions: FTIR spectroscopy was proven to rapidly detect the presence of porcine and bovine gelatins in dental prophylaxis paste and it was found that, sample A and sample C contains porcine gelatins while sample B and sample D contains bovine gelatins. 2015-06-12 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/43510/1/43510.pdf Tajudin, Noor Ashikin and Abd Aziz, Nur Fatin and Lestari, Widya and Ardini, Yunita Dewi (2015) Rapid detection of porcine and bovine gelatin in dental prophylaxis pastes using fourir transform infrared spectroscopy. In: MalaysiaiInternational Dental Exhibition and Conference 2015 (MIDEC 2015), 12th-14th June 2015, Kuala Lumpur Convention Center. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RK Dentistry
spellingShingle	RK Dentistry Tajudin, Noor Ashikin Abd Aziz, Nur Fatin Lestari, Widya Ardini, Yunita Dewi Rapid detection of porcine and bovine gelatin in dental prophylaxis pastes using fourir transform infrared spectroscopy
description	Aims: To detect the presence of porcine or bovine gelatins in dental prophylaxis pastes used in Malaysia by using Fourier Transform Infrared Spectroscopy (FTIR). Materials and Methods: Four brands of commonly used dental prophylaxis paste in Malaysia were collected and labelled A, B, C, and D. Pure porcine and bovine skin gelatins were used as controls. Gelatin extraction was carried out using acetone precipitation method. The extracts were then placed in the FTIR Perkin Elmer spectrometer for less than 5 minutes. All spectra were recorded within 4000– 400 cm-1 wavenumber with 4 cm-1 resolution under 40 scans. The results were analyzed by comparing the samples’ spectra with the controls’. The data were subjected to discriminant analysis using The SpectrumTM Software. Results: Sample A and porcine gelatin spectra had similar peak at 2163 cm-1 while sample B showed similarity to bovine gelatin at the range of 4000 – 2000 cm-1. Detailed analysis of the spectrum patterns of sample C in the area between 1600-1400 cm-1 and 3600-2800 cm-1 indicated an appearance of similar peaks to porcine gelatin. On the other hand, the spectrum of sample D at the range of 1800-1200 cm-1 is similar to that of bovine gelatin. Conclusions: FTIR spectroscopy was proven to rapidly detect the presence of porcine and bovine gelatins in dental prophylaxis paste and it was found that, sample A and sample C contains porcine gelatins while sample B and sample D contains bovine gelatins.
format	Conference or Workshop Item
author	Tajudin, Noor Ashikin Abd Aziz, Nur Fatin Lestari, Widya Ardini, Yunita Dewi
author_facet	Tajudin, Noor Ashikin Abd Aziz, Nur Fatin Lestari, Widya Ardini, Yunita Dewi
author_sort	Tajudin, Noor Ashikin
title	Rapid detection of porcine and bovine gelatin in dental prophylaxis pastes using fourir transform infrared spectroscopy
title_short	Rapid detection of porcine and bovine gelatin in dental prophylaxis pastes using fourir transform infrared spectroscopy
title_full	Rapid detection of porcine and bovine gelatin in dental prophylaxis pastes using fourir transform infrared spectroscopy
title_fullStr	Rapid detection of porcine and bovine gelatin in dental prophylaxis pastes using fourir transform infrared spectroscopy
title_full_unstemmed	Rapid detection of porcine and bovine gelatin in dental prophylaxis pastes using fourir transform infrared spectroscopy
title_sort	rapid detection of porcine and bovine gelatin in dental prophylaxis pastes using fourir transform infrared spectroscopy
publishDate	2015
url	http://irep.iium.edu.my/43510/ http://irep.iium.edu.my/43510/1/43510.pdf
first_indexed	2023-09-18T21:01:59Z
last_indexed	2023-09-18T21:01:59Z
_version_	1777410684573712384

Rapid detection of porcine and bovine gelatin in dental prophylaxis pastes using fourir transform infrared spectroscopy

Similar Items