Evaluation of PCR-based approach for serotype determination of streptococcus pneumoniae

Evaluation of PCR-based approach for serotype determination of streptococcus pneumoniae

Determination of Streptococcus pneumoniae serotypes is essential for epidemiological surveillance. Therefore accurate, reliable and cost effective serotyping method is crucial. In this study, we determined the serotypes of 41 pneumococcal isolates recovered from human anterior nares by multiplex Pol...

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Main Authors: Mohamed Shakrin, Nik Noorul Shakira, Balasubramaniam, Shandra Devi, Ahmad Yusof, Hanani, Mastuki, Mohd Fahmi, Masri, Siti Norbaya, Mohd Taib, Niazlin, Amin Nordin, Syafinaz, Jamal, Farida, Clarke, Stuart Charles, Mohd Desa, Mohd Nasir
Format: Article
Language:English
Published: Malaysian Society of Parasitology and Tropical Medicine 2013
Subjects:
QR Microbiology
Online Access:http://irep.iium.edu.my/43517/
http://irep.iium.edu.my/43517/
http://irep.iium.edu.my/43517/1/evaluation_of_pcr_based_approach_for_serotype_determination_of_streptococcus_pneumoniae.pdf
id iium-43517
recordtype eprints
spelling iium-435172015-06-24T06:53:14Z http://irep.iium.edu.my/43517/ Evaluation of PCR-based approach for serotype determination of streptococcus pneumoniae Mohamed Shakrin, Nik Noorul Shakira Balasubramaniam, Shandra Devi Ahmad Yusof, Hanani Mastuki, Mohd Fahmi Masri, Siti Norbaya Mohd Taib, Niazlin Amin Nordin, Syafinaz Jamal, Farida Clarke, Stuart Charles Mohd Desa, Mohd Nasir QR Microbiology Determination of Streptococcus pneumoniae serotypes is essential for epidemiological surveillance. Therefore accurate, reliable and cost effective serotyping method is crucial. In this study, we determined the serotypes of 41 pneumococcal isolates recovered from human anterior nares by multiplex Polymerase Chain Reaction (PCR) utilizing published primers. The data was then compared with conventional serology using latex agglutination (LA) and the Quellung reaction. Based on the PCR-approach, 8 different serogroups/serotypes were detected with one isolate classified as non-typeable (cpsAnegative). In reference to the serology-based data, the results were in agreement except for one isolate. For the latter isolate, the LA and Quellung tests failed to show a reaction but the PCR-approach and sequencing identified the isolate as serogroup 15B/C. Based on this experimental setting, we found that the PCR-approach for pneumococcal serotypes determination is reliable to serve as the alternative for determining the pneumococcal serotyping. Malaysian Society of Parasitology and Tropical Medicine 2013 Article PeerReviewed application/pdf en http://irep.iium.edu.my/43517/1/evaluation_of_pcr_based_approach_for_serotype_determination_of_streptococcus_pneumoniae.pdf Mohamed Shakrin, Nik Noorul Shakira and Balasubramaniam, Shandra Devi and Ahmad Yusof, Hanani and Mastuki, Mohd Fahmi and Masri, Siti Norbaya and Mohd Taib, Niazlin and Amin Nordin, Syafinaz and Jamal, Farida and Clarke, Stuart Charles and Mohd Desa, Mohd Nasir (2013) Evaluation of PCR-based approach for serotype determination of streptococcus pneumoniae. Tropical Biomedicine, 30 (2). pp. 338-344. ISSN 0127-5720, OCLC: 12391449 http://www.myjurnal.my/public/issue-view.php?id=4110&journal_id=78
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic QR Microbiology
spellingShingle QR Microbiology
Mohamed Shakrin, Nik Noorul Shakira
Balasubramaniam, Shandra Devi
Ahmad Yusof, Hanani
Mastuki, Mohd Fahmi
Masri, Siti Norbaya
Mohd Taib, Niazlin
Amin Nordin, Syafinaz
Jamal, Farida
Clarke, Stuart Charles
Mohd Desa, Mohd Nasir
Evaluation of PCR-based approach for serotype determination of streptococcus pneumoniae
description Determination of Streptococcus pneumoniae serotypes is essential for epidemiological surveillance. Therefore accurate, reliable and cost effective serotyping method is crucial. In this study, we determined the serotypes of 41 pneumococcal isolates recovered from human anterior nares by multiplex Polymerase Chain Reaction (PCR) utilizing published primers. The data was then compared with conventional serology using latex agglutination (LA) and the Quellung reaction. Based on the PCR-approach, 8 different serogroups/serotypes were detected with one isolate classified as non-typeable (cpsAnegative). In reference to the serology-based data, the results were in agreement except for one isolate. For the latter isolate, the LA and Quellung tests failed to show a reaction but the PCR-approach and sequencing identified the isolate as serogroup 15B/C. Based on this experimental setting, we found that the PCR-approach for pneumococcal serotypes determination is reliable to serve as the alternative for determining the pneumococcal serotyping.
format Article
author Mohamed Shakrin, Nik Noorul Shakira
Balasubramaniam, Shandra Devi
Ahmad Yusof, Hanani
Mastuki, Mohd Fahmi
Masri, Siti Norbaya
Mohd Taib, Niazlin
Amin Nordin, Syafinaz
Jamal, Farida
Clarke, Stuart Charles
Mohd Desa, Mohd Nasir
author_facet Mohamed Shakrin, Nik Noorul Shakira
Balasubramaniam, Shandra Devi
Ahmad Yusof, Hanani
Mastuki, Mohd Fahmi
Masri, Siti Norbaya
Mohd Taib, Niazlin
Amin Nordin, Syafinaz
Jamal, Farida
Clarke, Stuart Charles
Mohd Desa, Mohd Nasir
author_sort Mohamed Shakrin, Nik Noorul Shakira
title Evaluation of PCR-based approach for serotype determination of streptococcus pneumoniae
title_short Evaluation of PCR-based approach for serotype determination of streptococcus pneumoniae
title_full Evaluation of PCR-based approach for serotype determination of streptococcus pneumoniae
title_fullStr Evaluation of PCR-based approach for serotype determination of streptococcus pneumoniae
title_full_unstemmed Evaluation of PCR-based approach for serotype determination of streptococcus pneumoniae
title_sort evaluation of pcr-based approach for serotype determination of streptococcus pneumoniae
publisher Malaysian Society of Parasitology and Tropical Medicine
publishDate 2013
url http://irep.iium.edu.my/43517/
http://irep.iium.edu.my/43517/
http://irep.iium.edu.my/43517/1/evaluation_of_pcr_based_approach_for_serotype_determination_of_streptococcus_pneumoniae.pdf
first_indexed 2023-09-18T21:02:00Z
last_indexed 2023-09-18T21:02:00Z
_version_ 1777410685439836160

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