Detection of p16INK4a (p16) methylation in diffuse large B-cell lymphoma using methylation-specific PCR

Diffuse large B-cell lymphoma (DLBCL) is an aggressive malignancy of non-Hodgkin lymphoma, mostly involve lymph nodes. It is the most common lymphoma among adult with median age 70 years.p16is a tumour suppressor gene (TSG) which inhibits cyclin-dependent kinase thus inactivatesRb protein and block...

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Main Authors:	Mohd. Ridah, Lailatul Jalilah, Zainuddin, Norafiza
Format:	Conference or Workshop Item
Language:	English
Published:	2014
Subjects:	RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Online Access:	http://irep.iium.edu.my/43616/ http://irep.iium.edu.my/43616/4/IHCI_2014.pdf

id	iium-43616
recordtype	eprints
spelling	iium-436162018-05-23T06:05:02Z http://irep.iium.edu.my/43616/ Detection of p16INK4a (p16) methylation in diffuse large B-cell lymphoma using methylation-specific PCR Mohd. Ridah, Lailatul Jalilah Zainuddin, Norafiza RC0254 Neoplasms. Tumors. Oncology (including Cancer) Diffuse large B-cell lymphoma (DLBCL) is an aggressive malignancy of non-Hodgkin lymphoma, mostly involve lymph nodes. It is the most common lymphoma among adult with median age 70 years.p16is a tumour suppressor gene (TSG) which inhibits cyclin-dependent kinase thus inactivatesRb protein and blockade G1 phase. Silence of p16 induced by DNA methylation epigenetically has been reported as one of the factors toward DLBCL occurrence with varying percentages. Loss of p16 protein causes unregulated cell division which may lead to cancer. In this study, we aim to identify the presence of p16 methylation in DLBCL. DLBCL samples were obtained from two study groups; Kelantan and Pahang states. Methylation-specific PCR (MSP) was utilized to detect p16 methylation using specific primers in 39 formalin-fixed paraffin embedded tissues samples. The PCR products were then visualized on 2% agarose gels. 14 of 34 (41%) samples are found to have p16 methylation.There was no p16 methylation detected in five normal thyroid samples. p16 gene methylation has been detected in almost half of our samples. This could provide data of gene methylation in DLBCL among Malaysian. Thus, more studies should be carried out for further investigation. 2014-12-04 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/43616/4/IHCI_2014.pdf Mohd. Ridah, Lailatul Jalilah and Zainuddin, Norafiza (2014) Detection of p16INK4a (p16) methylation in diffuse large B-cell lymphoma using methylation-specific PCR. In: International Health Conference IIUM 2014, 3-4 Dec 2014, Kuantan, Pahang. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RC0254 Neoplasms. Tumors. Oncology (including Cancer)
spellingShingle	RC0254 Neoplasms. Tumors. Oncology (including Cancer) Mohd. Ridah, Lailatul Jalilah Zainuddin, Norafiza Detection of p16INK4a (p16) methylation in diffuse large B-cell lymphoma using methylation-specific PCR
description	Diffuse large B-cell lymphoma (DLBCL) is an aggressive malignancy of non-Hodgkin lymphoma, mostly involve lymph nodes. It is the most common lymphoma among adult with median age 70 years.p16is a tumour suppressor gene (TSG) which inhibits cyclin-dependent kinase thus inactivatesRb protein and blockade G1 phase. Silence of p16 induced by DNA methylation epigenetically has been reported as one of the factors toward DLBCL occurrence with varying percentages. Loss of p16 protein causes unregulated cell division which may lead to cancer. In this study, we aim to identify the presence of p16 methylation in DLBCL. DLBCL samples were obtained from two study groups; Kelantan and Pahang states. Methylation-specific PCR (MSP) was utilized to detect p16 methylation using specific primers in 39 formalin-fixed paraffin embedded tissues samples. The PCR products were then visualized on 2% agarose gels. 14 of 34 (41%) samples are found to have p16 methylation.There was no p16 methylation detected in five normal thyroid samples. p16 gene methylation has been detected in almost half of our samples. This could provide data of gene methylation in DLBCL among Malaysian. Thus, more studies should be carried out for further investigation.
format	Conference or Workshop Item
author	Mohd. Ridah, Lailatul Jalilah Zainuddin, Norafiza
author_facet	Mohd. Ridah, Lailatul Jalilah Zainuddin, Norafiza
author_sort	Mohd. Ridah, Lailatul Jalilah
title	Detection of p16INK4a (p16) methylation in diffuse large B-cell lymphoma using methylation-specific PCR
title_short	Detection of p16INK4a (p16) methylation in diffuse large B-cell lymphoma using methylation-specific PCR
title_full	Detection of p16INK4a (p16) methylation in diffuse large B-cell lymphoma using methylation-specific PCR
title_fullStr	Detection of p16INK4a (p16) methylation in diffuse large B-cell lymphoma using methylation-specific PCR
title_full_unstemmed	Detection of p16INK4a (p16) methylation in diffuse large B-cell lymphoma using methylation-specific PCR
title_sort	detection of p16ink4a (p16) methylation in diffuse large b-cell lymphoma using methylation-specific pcr
publishDate	2014
url	http://irep.iium.edu.my/43616/ http://irep.iium.edu.my/43616/4/IHCI_2014.pdf
first_indexed	2023-09-18T21:02:09Z
last_indexed	2023-09-18T21:02:09Z
_version_	1777410694678839296

Detection of p16INK4a (p16) methylation in diffuse large B-cell lymphoma using methylation-specific PCR

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