Preparation of decellularized meniscal scaffolds using sonication treatment for tissue engineering

Scaffolds play a key role in the process of regeneration and morphogenesis of tissue or organ. We have developed a novel sonication decellularization system to prepare decellularized bio-scaffolds in a short treatment time. The aim of the study is to investigate sonication decellularization conditio...

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Main Authors:	Noor Azmi, Azran Azhim, Ono, T, Fukui, Y., Morimoto, Y., Furukawa, Katsuko, Ushida, Takashi
Format:	Conference or Workshop Item
Language:	English
Published:	IEEE 2013
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/43657/ http://irep.iium.edu.my/43657/ http://irep.iium.edu.my/43657/ http://irep.iium.edu.my/43657/1/Preparation_of_decellularized_meniscal_scaffolds_using_sonication_treatment_for_tissue_engineering..pdf

id	iium-43657
recordtype	eprints
spelling	iium-436572015-08-04T06:40:26Z http://irep.iium.edu.my/43657/ Preparation of decellularized meniscal scaffolds using sonication treatment for tissue engineering Noor Azmi, Azran Azhim Ono, T Fukui, Y. Morimoto, Y. Furukawa, Katsuko Ushida, Takashi TP248.13 Biotechnology Scaffolds play a key role in the process of regeneration and morphogenesis of tissue or organ. We have developed a novel sonication decellularization system to prepare decellularized bio-scaffolds in a short treatment time. The aim of the study is to investigate sonication decellularization condition that completely decellularize meniscus can be changed as well as to maintain the biomechanical parameters of scaffolds. The meniscus samples were decellularized using sonication treatment. The treated samples were evaluated histologically by EVG for cell removal, picrosirius red for content of collagen type I and III, and safranin-O/fast green staining for content of glycosaminoglycan, and SEM for observation of scaffold surface. Indentation apparatus was used to analyze the unconfined deformation under load of native and decellularized menisci. The load parameters which are stiffness, compression and residual force were not significantly different compare with native and sonicated scaffolds. However, the content of extracellular matrix and its fiber alignment changed significantly due to sonication treatment as observed by SEM and safranin-O/fast green staining, respectively. The removal of immunogenic cell components by sonication decellularization as well as maintain its biomechanical strength of decellularized scaffolds, so that it has potential to use as an implant material for tissue engineering of menisci. IEEE 2013 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/43657/1/Preparation_of_decellularized_meniscal_scaffolds_using_sonication_treatment_for_tissue_engineering..pdf Noor Azmi, Azran Azhim and Ono, T and Fukui, Y. and Morimoto, Y. and Furukawa, Katsuko and Ushida, Takashi (2013) Preparation of decellularized meniscal scaffolds using sonication treatment for tissue engineering. In: The 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC’13), 3-7 July 2013, Osaka International Convention Center, in Osaka, Japan. http://ieeexplore.ieee.org/xpl/articleDetails.jsp?arnumber=6611157 DOI:10.1109/EMBC.2013.6611157
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Noor Azmi, Azran Azhim Ono, T Fukui, Y. Morimoto, Y. Furukawa, Katsuko Ushida, Takashi Preparation of decellularized meniscal scaffolds using sonication treatment for tissue engineering
description	Scaffolds play a key role in the process of regeneration and morphogenesis of tissue or organ. We have developed a novel sonication decellularization system to prepare decellularized bio-scaffolds in a short treatment time. The aim of the study is to investigate sonication decellularization condition that completely decellularize meniscus can be changed as well as to maintain the biomechanical parameters of scaffolds. The meniscus samples were decellularized using sonication treatment. The treated samples were evaluated histologically by EVG for cell removal, picrosirius red for content of collagen type I and III, and safranin-O/fast green staining for content of glycosaminoglycan, and SEM for observation of scaffold surface. Indentation apparatus was used to analyze the unconfined deformation under load of native and decellularized menisci. The load parameters which are stiffness, compression and residual force were not significantly different compare with native and sonicated scaffolds. However, the content of extracellular matrix and its fiber alignment changed significantly due to sonication treatment as observed by SEM and safranin-O/fast green staining, respectively. The removal of immunogenic cell components by sonication decellularization as well as maintain its biomechanical strength of decellularized scaffolds, so that it has potential to use as an implant material for tissue engineering of menisci.
format	Conference or Workshop Item
author	Noor Azmi, Azran Azhim Ono, T Fukui, Y. Morimoto, Y. Furukawa, Katsuko Ushida, Takashi
author_facet	Noor Azmi, Azran Azhim Ono, T Fukui, Y. Morimoto, Y. Furukawa, Katsuko Ushida, Takashi
author_sort	Noor Azmi, Azran Azhim
title	Preparation of decellularized meniscal scaffolds using sonication treatment for tissue engineering
title_short	Preparation of decellularized meniscal scaffolds using sonication treatment for tissue engineering
title_full	Preparation of decellularized meniscal scaffolds using sonication treatment for tissue engineering
title_fullStr	Preparation of decellularized meniscal scaffolds using sonication treatment for tissue engineering
title_full_unstemmed	Preparation of decellularized meniscal scaffolds using sonication treatment for tissue engineering
title_sort	preparation of decellularized meniscal scaffolds using sonication treatment for tissue engineering
publisher	IEEE
publishDate	2013
url	http://irep.iium.edu.my/43657/ http://irep.iium.edu.my/43657/ http://irep.iium.edu.my/43657/ http://irep.iium.edu.my/43657/1/Preparation_of_decellularized_meniscal_scaffolds_using_sonication_treatment_for_tissue_engineering..pdf
first_indexed	2023-09-18T21:02:09Z
last_indexed	2023-09-18T21:02:09Z
_version_	1777410695253458944

Preparation of decellularized meniscal scaffolds using sonication treatment for tissue engineering

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