Qualitative detection of p16INK4a tumor suppressor gene methylation in diffuse large b-cell lymphoma using methylation-specific PCR

INTRODUCTION: Diffuse large B-cell lymphoma (DLBCL) is an aggressive malignancy of non-Hodgkin lymphoma, mostly involve lymph nodes. It is the most common lymphoma among adult with median age 70 years. p16 is a tumour suppressor gene (TSG) which inhibits cyclin-dependent kinase thus inactivates Rb p...

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Main Authors:	Mohd. Ridah, Lailatul Jalilah, Md Dzali, Nurul Balqis, Zainuddin, Norafiza
Format:	Conference or Workshop Item
Language:	English English
Published:	2014
Subjects:	RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Online Access:	http://irep.iium.edu.my/43663/ http://irep.iium.edu.my/43663/ http://irep.iium.edu.my/43663/1/KAHS_RESEARCH_WEEK_2014_ABSTRACT_BOOK.pdf http://irep.iium.edu.my/43663/4/43663.pdf

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spelling	iium-436632015-07-30T02:41:36Z http://irep.iium.edu.my/43663/ Qualitative detection of p16INK4a tumor suppressor gene methylation in diffuse large b-cell lymphoma using methylation-specific PCR Mohd. Ridah, Lailatul Jalilah Md Dzali, Nurul Balqis Zainuddin, Norafiza RC0254 Neoplasms. Tumors. Oncology (including Cancer) INTRODUCTION: Diffuse large B-cell lymphoma (DLBCL) is an aggressive malignancy of non-Hodgkin lymphoma, mostly involve lymph nodes. It is the most common lymphoma among adult with median age 70 years. p16 is a tumour suppressor gene (TSG) which inhibits cyclin-dependent kinase thus inactivates Rb protein and blockade G1 phase. Silence of p16 induced by DNA methylation epigenetically has been reported as one of the factors toward DLBCL occurrence with varying percentages. Loss of p16 protein causes unregulated cell division which may lead to cancer. In this study, we aim to identify the presence of P16INK4a gene methylation in DLBCL and to correlate the methylation status with patients’ age and gender. MATERIALS AND METHODS: The samples of DLBCL were retrieved from two study groups; Kelantan and Pahang states. Methylation-specific PCR (MSP) was utilized to detect p16 methylation using specific primers in 88 formalin-fixed paraffin embedded DLBCL tissues including five normal thyroid samples. The PCR products were then visualized on 2% agarose gels under UV illumination. RESULTS AND DISCUSSION: 64 of 88 (73%) samples were found to have p16 methylation. There was no p16 methylation spotted in five normal thyroid samples. We found statistically significant association between p16 methylation and patients’ age (>50 years) with p = 0.023. While, there is no association between p16 methylation and gender observed in this study. CONCLUSION: p16 gene methylation has been detected in most of the samples with 72% of methylation occurred among patients more than 50 years. This could provide data of gene methylation in DLBCL among Malaysian. Thus, more studies should be carried out for further investigation. 2014 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/43663/1/KAHS_RESEARCH_WEEK_2014_ABSTRACT_BOOK.pdf application/pdf en http://irep.iium.edu.my/43663/4/43663.pdf Mohd. Ridah, Lailatul Jalilah and Md Dzali, Nurul Balqis and Zainuddin, Norafiza (2014) Qualitative detection of p16INK4a tumor suppressor gene methylation in diffuse large b-cell lymphoma using methylation-specific PCR. In: KAHS Research Week, 17 - 21 November 2014, KAHS Examination Hall & Lecture Hall 1. http://www.iium.edu.my/kahs/events/kahs-research-week-2014
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RC0254 Neoplasms. Tumors. Oncology (including Cancer)
spellingShingle	RC0254 Neoplasms. Tumors. Oncology (including Cancer) Mohd. Ridah, Lailatul Jalilah Md Dzali, Nurul Balqis Zainuddin, Norafiza Qualitative detection of p16INK4a tumor suppressor gene methylation in diffuse large b-cell lymphoma using methylation-specific PCR
description	INTRODUCTION: Diffuse large B-cell lymphoma (DLBCL) is an aggressive malignancy of non-Hodgkin lymphoma, mostly involve lymph nodes. It is the most common lymphoma among adult with median age 70 years. p16 is a tumour suppressor gene (TSG) which inhibits cyclin-dependent kinase thus inactivates Rb protein and blockade G1 phase. Silence of p16 induced by DNA methylation epigenetically has been reported as one of the factors toward DLBCL occurrence with varying percentages. Loss of p16 protein causes unregulated cell division which may lead to cancer. In this study, we aim to identify the presence of P16INK4a gene methylation in DLBCL and to correlate the methylation status with patients’ age and gender. MATERIALS AND METHODS: The samples of DLBCL were retrieved from two study groups; Kelantan and Pahang states. Methylation-specific PCR (MSP) was utilized to detect p16 methylation using specific primers in 88 formalin-fixed paraffin embedded DLBCL tissues including five normal thyroid samples. The PCR products were then visualized on 2% agarose gels under UV illumination. RESULTS AND DISCUSSION: 64 of 88 (73%) samples were found to have p16 methylation. There was no p16 methylation spotted in five normal thyroid samples. We found statistically significant association between p16 methylation and patients’ age (>50 years) with p = 0.023. While, there is no association between p16 methylation and gender observed in this study. CONCLUSION: p16 gene methylation has been detected in most of the samples with 72% of methylation occurred among patients more than 50 years. This could provide data of gene methylation in DLBCL among Malaysian. Thus, more studies should be carried out for further investigation.
format	Conference or Workshop Item
author	Mohd. Ridah, Lailatul Jalilah Md Dzali, Nurul Balqis Zainuddin, Norafiza
author_facet	Mohd. Ridah, Lailatul Jalilah Md Dzali, Nurul Balqis Zainuddin, Norafiza
author_sort	Mohd. Ridah, Lailatul Jalilah
title	Qualitative detection of p16INK4a tumor suppressor gene methylation in diffuse large b-cell lymphoma using methylation-specific PCR
title_short	Qualitative detection of p16INK4a tumor suppressor gene methylation in diffuse large b-cell lymphoma using methylation-specific PCR
title_full	Qualitative detection of p16INK4a tumor suppressor gene methylation in diffuse large b-cell lymphoma using methylation-specific PCR
title_fullStr	Qualitative detection of p16INK4a tumor suppressor gene methylation in diffuse large b-cell lymphoma using methylation-specific PCR
title_full_unstemmed	Qualitative detection of p16INK4a tumor suppressor gene methylation in diffuse large b-cell lymphoma using methylation-specific PCR
title_sort	qualitative detection of p16ink4a tumor suppressor gene methylation in diffuse large b-cell lymphoma using methylation-specific pcr
publishDate	2014
url	http://irep.iium.edu.my/43663/ http://irep.iium.edu.my/43663/ http://irep.iium.edu.my/43663/1/KAHS_RESEARCH_WEEK_2014_ABSTRACT_BOOK.pdf http://irep.iium.edu.my/43663/4/43663.pdf
first_indexed	2023-09-18T21:02:10Z
last_indexed	2023-09-18T21:02:10Z
_version_	1777410695673937920

Qualitative detection of p16INK4a tumor suppressor gene methylation in diffuse large b-cell lymphoma using methylation-specific PCR

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