Expression analysis of notch signaling pathway molecules in SHED cultured in keratinocyte growth medium

Aim: To detect the expression of molecules associated with Notch signaling pathway in stem cells from human exfoliated deciduous teeth (SHED) cultured in specific differentiation medium, namely, keratinocyte growth medium (KGM). Methods: RNA was extracted from SHED harvested on day 1, 3 and 7. RN...

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Main Authors:	Mohd Taha, Siti Aisyah, Koh Su Ling, Joanne, Azmi, Nur Izyan, Thirumulu Ponnuraj, Kannan, Ahmad, Azlina, Mokhtar, Khairani Idah
Format:	Article
Language:	English English
Published:	Piracicaba Dental School, Brazil 2015
Subjects:	RK Dentistry
Online Access:	http://irep.iium.edu.my/43699/ http://irep.iium.edu.my/43699/ http://irep.iium.edu.my/43699/1/2015-BJOS-_Expression_analysis_of_Notch_signaling_pathway_molecules_in_SHED.pdf http://irep.iium.edu.my/43699/4/43699_Expression%20analysis%20of%20notch%20signaling%20pathway_SCOPUS.pdf

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spelling	iium-436992017-11-09T04:20:19Z http://irep.iium.edu.my/43699/ Expression analysis of notch signaling pathway molecules in SHED cultured in keratinocyte growth medium Mohd Taha, Siti Aisyah Koh Su Ling, Joanne Azmi, Nur Izyan Thirumulu Ponnuraj, Kannan Ahmad, Azlina Mokhtar, Khairani Idah RK Dentistry Aim: To detect the expression of molecules associated with Notch signaling pathway in stem cells from human exfoliated deciduous teeth (SHED) cultured in specific differentiation medium, namely, keratinocyte growth medium (KGM). Methods: RNA was extracted from SHED harvested on day 1, 3 and 7. RNA was reverse-transcribed to obtain the cDNA and then proceeded with PCR using specific primers for the Notch signaling pathway molecules (Notch1, Jagged-1, Jagged-2 and, Hes1) as well as stem cell marker (Nanog). PCR products were electrophoresed on a 2% agarose gel and stained with SYBR green. Results: Notch-1 was highly expressed in SHED cultured in KGM and showed increase in density as the days progressed, while Jagged-1 showed a decrease. Jagged-2 on the other hand, showed a slight increase on day 3 followed by a decrease on day 7. However, Hes-1 was not expressed in SHED cultured in KGM. Nanog showed expression only on day 3 and gradually increased in expression on day 7. Conclusions: Notch signaling pathway associated molecules; Notch-1, Jagged-1, Jagged-2, and stem cell marker Nanog are expressed in SHED cultured in KGM which may be involved in the differentiation into epithelial-like cells in human dental pulp tissues. Keywords: receptors, notch; gene expression; stem cells; tooth, deciduous; culture media. Piracicaba Dental School, Brazil 2015-06-09 Article PeerReviewed application/pdf en http://irep.iium.edu.my/43699/1/2015-BJOS-_Expression_analysis_of_Notch_signaling_pathway_molecules_in_SHED.pdf application/pdf en http://irep.iium.edu.my/43699/4/43699_Expression%20analysis%20of%20notch%20signaling%20pathway_SCOPUS.pdf Mohd Taha, Siti Aisyah and Koh Su Ling, Joanne and Azmi, Nur Izyan and Thirumulu Ponnuraj, Kannan and Ahmad, Azlina and Mokhtar, Khairani Idah (2015) Expression analysis of notch signaling pathway molecules in SHED cultured in keratinocyte growth medium. Brazilian Journal of Oral Sciences, 14 (2). pp. 135-140. ISSN 1677-3225 http://www.scielo.br/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RK Dentistry
spellingShingle	RK Dentistry Mohd Taha, Siti Aisyah Koh Su Ling, Joanne Azmi, Nur Izyan Thirumulu Ponnuraj, Kannan Ahmad, Azlina Mokhtar, Khairani Idah Expression analysis of notch signaling pathway molecules in SHED cultured in keratinocyte growth medium
description	Aim: To detect the expression of molecules associated with Notch signaling pathway in stem cells from human exfoliated deciduous teeth (SHED) cultured in specific differentiation medium, namely, keratinocyte growth medium (KGM). Methods: RNA was extracted from SHED harvested on day 1, 3 and 7. RNA was reverse-transcribed to obtain the cDNA and then proceeded with PCR using specific primers for the Notch signaling pathway molecules (Notch1, Jagged-1, Jagged-2 and, Hes1) as well as stem cell marker (Nanog). PCR products were electrophoresed on a 2% agarose gel and stained with SYBR green. Results: Notch-1 was highly expressed in SHED cultured in KGM and showed increase in density as the days progressed, while Jagged-1 showed a decrease. Jagged-2 on the other hand, showed a slight increase on day 3 followed by a decrease on day 7. However, Hes-1 was not expressed in SHED cultured in KGM. Nanog showed expression only on day 3 and gradually increased in expression on day 7. Conclusions: Notch signaling pathway associated molecules; Notch-1, Jagged-1, Jagged-2, and stem cell marker Nanog are expressed in SHED cultured in KGM which may be involved in the differentiation into epithelial-like cells in human dental pulp tissues. Keywords: receptors, notch; gene expression; stem cells; tooth, deciduous; culture media.
format	Article
author	Mohd Taha, Siti Aisyah Koh Su Ling, Joanne Azmi, Nur Izyan Thirumulu Ponnuraj, Kannan Ahmad, Azlina Mokhtar, Khairani Idah
author_facet	Mohd Taha, Siti Aisyah Koh Su Ling, Joanne Azmi, Nur Izyan Thirumulu Ponnuraj, Kannan Ahmad, Azlina Mokhtar, Khairani Idah
author_sort	Mohd Taha, Siti Aisyah
title	Expression analysis of notch signaling pathway molecules in SHED cultured in keratinocyte growth medium
title_short	Expression analysis of notch signaling pathway molecules in SHED cultured in keratinocyte growth medium
title_full	Expression analysis of notch signaling pathway molecules in SHED cultured in keratinocyte growth medium
title_fullStr	Expression analysis of notch signaling pathway molecules in SHED cultured in keratinocyte growth medium
title_full_unstemmed	Expression analysis of notch signaling pathway molecules in SHED cultured in keratinocyte growth medium
title_sort	expression analysis of notch signaling pathway molecules in shed cultured in keratinocyte growth medium
publisher	Piracicaba Dental School, Brazil
publishDate	2015
url	http://irep.iium.edu.my/43699/ http://irep.iium.edu.my/43699/ http://irep.iium.edu.my/43699/1/2015-BJOS-_Expression_analysis_of_Notch_signaling_pathway_molecules_in_SHED.pdf http://irep.iium.edu.my/43699/4/43699_Expression%20analysis%20of%20notch%20signaling%20pathway_SCOPUS.pdf
first_indexed	2023-09-18T21:02:13Z
last_indexed	2023-09-18T21:02:13Z
_version_	1777410698763042816

Expression analysis of notch signaling pathway molecules in SHED cultured in keratinocyte growth medium

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