Decellularization of aorta tissue using sonication treatment as potential scaffold for vascular tissue engineering

Decellularization of aorta tissue using sonication treatment as potential scaffold for vascular tissue engineering

Arterial scaffolds have potential for replacing native arteries for vascular surgery. These scaffolds are anti-thrombogenic, biocompatible, and capable of growth and repair, making them suitable for application in vascular tissue engineering. This study develops a sonication decellularization system...

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Main Authors: Nurul , Syazwani, Noor Azmi, Azran Azhim, Yuji, Morimoto, Takashi , Ushida, Katsuko , Furukawa
Format: Article
Language:English
Published: Springer Berlin Heidelberg 2015
Subjects:
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/43793/
http://irep.iium.edu.my/43793/
http://irep.iium.edu.my/43793/
http://irep.iium.edu.my/43793/1/jmbe_paper-author%27s_copy.pdf
id iium-43793
recordtype eprints
spelling iium-437932017-11-01T08:42:10Z http://irep.iium.edu.my/43793/ Decellularization of aorta tissue using sonication treatment as potential scaffold for vascular tissue engineering Nurul , Syazwani Noor Azmi, Azran Azhim Yuji, Morimoto Takashi , Ushida Katsuko , Furukawa TP248.13 Biotechnology Arterial scaffolds have potential for replacing native arteries for vascular surgery. These scaffolds are anti-thrombogenic, biocompatible, and capable of growth and repair, making them suitable for application in vascular tissue engineering. This study develops a sonication decellularization system for preparing a complete decellularized artery. The sonication decellularization efficiency on arterial tissues is investigated. Aorta samples are decellularized by sonication treatment with various treatment times and application of sodium dodecyl sulfate (SDS) detergent with and without saline. The relation between decellularization and dissolved oxygen concentration is investigated. The treated samples are evaluated using hematoxylin–eosin staining, scanning electron microscopy, diamidino-2-phenylindol (DAPI) staining, biomechanical testing, and DNA quantification. From the histological analysis, sonication treatment without saline shows complete decellularization at a specific region on the extracellular matrix. This is further confirmed by the DAPI staining, which demonstrates complete removal of DNA fragments for sonication treatment in 2 % SDS without saline. Sonication treatment without saline is thus capable of producing complete decellularized scaffolds for vascular tissue engineering. Springer Berlin Heidelberg 2015-03-26 Article PeerReviewed application/pdf en http://irep.iium.edu.my/43793/1/jmbe_paper-author%27s_copy.pdf Nurul , Syazwani and Noor Azmi, Azran Azhim and Yuji, Morimoto and Takashi , Ushida and Katsuko , Furukawa (2015) Decellularization of aorta tissue using sonication treatment as potential scaffold for vascular tissue engineering. Journal of Medical and Biological Engineering, 35 (2). pp. 258-269. ISSN 1609-0985 http://link.springer.com/article/10.1007%2Fs40846-015-0028-5 10.1007/s40846-015-0028-5
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic TP248.13 Biotechnology
spellingShingle TP248.13 Biotechnology
Nurul , Syazwani
Noor Azmi, Azran Azhim
Yuji, Morimoto
Takashi , Ushida
Katsuko , Furukawa
Decellularization of aorta tissue using sonication treatment as potential scaffold for vascular tissue engineering
description Arterial scaffolds have potential for replacing native arteries for vascular surgery. These scaffolds are anti-thrombogenic, biocompatible, and capable of growth and repair, making them suitable for application in vascular tissue engineering. This study develops a sonication decellularization system for preparing a complete decellularized artery. The sonication decellularization efficiency on arterial tissues is investigated. Aorta samples are decellularized by sonication treatment with various treatment times and application of sodium dodecyl sulfate (SDS) detergent with and without saline. The relation between decellularization and dissolved oxygen concentration is investigated. The treated samples are evaluated using hematoxylin–eosin staining, scanning electron microscopy, diamidino-2-phenylindol (DAPI) staining, biomechanical testing, and DNA quantification. From the histological analysis, sonication treatment without saline shows complete decellularization at a specific region on the extracellular matrix. This is further confirmed by the DAPI staining, which demonstrates complete removal of DNA fragments for sonication treatment in 2 % SDS without saline. Sonication treatment without saline is thus capable of producing complete decellularized scaffolds for vascular tissue engineering.
format Article
author Nurul , Syazwani
Noor Azmi, Azran Azhim
Yuji, Morimoto
Takashi , Ushida
Katsuko , Furukawa
author_facet Nurul , Syazwani
Noor Azmi, Azran Azhim
Yuji, Morimoto
Takashi , Ushida
Katsuko , Furukawa
author_sort Nurul , Syazwani
title Decellularization of aorta tissue using sonication treatment as potential scaffold for vascular tissue engineering
title_short Decellularization of aorta tissue using sonication treatment as potential scaffold for vascular tissue engineering
title_full Decellularization of aorta tissue using sonication treatment as potential scaffold for vascular tissue engineering
title_fullStr Decellularization of aorta tissue using sonication treatment as potential scaffold for vascular tissue engineering
title_full_unstemmed Decellularization of aorta tissue using sonication treatment as potential scaffold for vascular tissue engineering
title_sort decellularization of aorta tissue using sonication treatment as potential scaffold for vascular tissue engineering
publisher Springer Berlin Heidelberg
publishDate 2015
url http://irep.iium.edu.my/43793/
http://irep.iium.edu.my/43793/
http://irep.iium.edu.my/43793/
http://irep.iium.edu.my/43793/1/jmbe_paper-author%27s_copy.pdf
first_indexed 2023-09-18T21:02:20Z
last_indexed 2023-09-18T21:02:20Z
_version_ 1777410706881118208

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