The impact of acoustic intensity on solution parameters and decellularization using sonication treatment
A combination method using sonication and chemical treatment to decellularize tissue sample is described. The objective of the study is to determine the acoustic intensity and solution parameters modulating decellularization process. The acoustic intensity distribution of sonication system was fi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
American Scientific Publishers
2015
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/43804/ http://irep.iium.edu.my/43804/ http://irep.iium.edu.my/43804/ http://irep.iium.edu.my/43804/1/The_Impact_of_AcousticIntensity_on_Solution_Parameters.pdf |
| Summary: | A combination method using sonication and chemical treatment to decellularize tissue sample is
described. The objective of the study is to determine the acoustic intensity and solution parameters
modulating decellularization process. The acoustic intensity distribution of sonication system
was first demonstrated by simulation and followed by experimental measurement. The effect of
acoustic intensity with solution parameters including dissolved oxygen (DO) concentration, pH, and
conductivity was observed using 170 kHz system. From both simulation and experimental results,
it is demonstrated that acoustic intensity effects were localised as high cell removal recorded at
the center of irradiation. Furthermore, our study demonstrated strong correlation between acoustic
intensity with solution parameters. The study indicates positive correlation of DO concentration
and pH of SDS detergent with acoustic intensity and negative correlation between conductivity and
acoustic intensity. The results further supported by the complete removal of DNA with preservation
of extracellular matrix (ECM), collagen and elastin content. In conclusion, the effect of acoustic
intensity is localised and influenced decellularization. Our study also indicates strong correlation
between acoustic intensity and solution parameters as we suggested the formation of cavitation
bubble derived the decellularization process. |
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