The impact of acoustic intensity on solution parameters and decellularization using sonication treatment

A combination method using sonication and chemical treatment to decellularize tissue sample is described. The objective of the study is to determine the acoustic intensity and solution parameters modulating decellularization process. The acoustic intensity distribution of sonication system was fi...

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Main Authors:	Noor Azmi, Azran Azhim, Shafiq, Mohammad, Rasyada, A. Rahman, Furukawa, Katsuko, Ushida, Takashi
Format:	Article
Language:	English
Published:	American Scientific Publishers 2015
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/43804/ http://irep.iium.edu.my/43804/ http://irep.iium.edu.my/43804/ http://irep.iium.edu.my/43804/1/The_Impact_of_AcousticIntensity_on_Solution_Parameters.pdf

id	iium-43804
recordtype	eprints
spelling	iium-438042018-06-26T03:17:20Z http://irep.iium.edu.my/43804/ The impact of acoustic intensity on solution parameters and decellularization using sonication treatment Noor Azmi, Azran Azhim Shafiq, Mohammad Rasyada, A. Rahman Furukawa, Katsuko Ushida, Takashi TP248.13 Biotechnology A combination method using sonication and chemical treatment to decellularize tissue sample is described. The objective of the study is to determine the acoustic intensity and solution parameters modulating decellularization process. The acoustic intensity distribution of sonication system was first demonstrated by simulation and followed by experimental measurement. The effect of acoustic intensity with solution parameters including dissolved oxygen (DO) concentration, pH, and conductivity was observed using 170 kHz system. From both simulation and experimental results, it is demonstrated that acoustic intensity effects were localised as high cell removal recorded at the center of irradiation. Furthermore, our study demonstrated strong correlation between acoustic intensity with solution parameters. The study indicates positive correlation of DO concentration and pH of SDS detergent with acoustic intensity and negative correlation between conductivity and acoustic intensity. The results further supported by the complete removal of DNA with preservation of extracellular matrix (ECM), collagen and elastin content. In conclusion, the effect of acoustic intensity is localised and influenced decellularization. Our study also indicates strong correlation between acoustic intensity and solution parameters as we suggested the formation of cavitation bubble derived the decellularization process. American Scientific Publishers 2015 Article PeerReviewed application/pdf en http://irep.iium.edu.my/43804/1/The_Impact_of_AcousticIntensity_on_Solution_Parameters.pdf Noor Azmi, Azran Azhim and Shafiq, Mohammad and Rasyada, A. Rahman and Furukawa, Katsuko and Ushida, Takashi (2015) The impact of acoustic intensity on solution parameters and decellularization using sonication treatment. Journal of Biomaterials and Tissue Engineering , 5 (xx). pp. 1-9. ISSN 2157-9083 http://www.aspbs.com/jbt/contents_jbt2015.htm 10.1166/jbt.2015.1300
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Noor Azmi, Azran Azhim Shafiq, Mohammad Rasyada, A. Rahman Furukawa, Katsuko Ushida, Takashi The impact of acoustic intensity on solution parameters and decellularization using sonication treatment
description	A combination method using sonication and chemical treatment to decellularize tissue sample is described. The objective of the study is to determine the acoustic intensity and solution parameters modulating decellularization process. The acoustic intensity distribution of sonication system was first demonstrated by simulation and followed by experimental measurement. The effect of acoustic intensity with solution parameters including dissolved oxygen (DO) concentration, pH, and conductivity was observed using 170 kHz system. From both simulation and experimental results, it is demonstrated that acoustic intensity effects were localised as high cell removal recorded at the center of irradiation. Furthermore, our study demonstrated strong correlation between acoustic intensity with solution parameters. The study indicates positive correlation of DO concentration and pH of SDS detergent with acoustic intensity and negative correlation between conductivity and acoustic intensity. The results further supported by the complete removal of DNA with preservation of extracellular matrix (ECM), collagen and elastin content. In conclusion, the effect of acoustic intensity is localised and influenced decellularization. Our study also indicates strong correlation between acoustic intensity and solution parameters as we suggested the formation of cavitation bubble derived the decellularization process.
format	Article
author	Noor Azmi, Azran Azhim Shafiq, Mohammad Rasyada, A. Rahman Furukawa, Katsuko Ushida, Takashi
author_facet	Noor Azmi, Azran Azhim Shafiq, Mohammad Rasyada, A. Rahman Furukawa, Katsuko Ushida, Takashi
author_sort	Noor Azmi, Azran Azhim
title	The impact of acoustic intensity on solution parameters and decellularization using sonication treatment
title_short	The impact of acoustic intensity on solution parameters and decellularization using sonication treatment
title_full	The impact of acoustic intensity on solution parameters and decellularization using sonication treatment
title_fullStr	The impact of acoustic intensity on solution parameters and decellularization using sonication treatment
title_full_unstemmed	The impact of acoustic intensity on solution parameters and decellularization using sonication treatment
title_sort	impact of acoustic intensity on solution parameters and decellularization using sonication treatment
publisher	American Scientific Publishers
publishDate	2015
url	http://irep.iium.edu.my/43804/ http://irep.iium.edu.my/43804/ http://irep.iium.edu.my/43804/ http://irep.iium.edu.my/43804/1/The_Impact_of_AcousticIntensity_on_Solution_Parameters.pdf
first_indexed	2023-09-18T21:02:21Z
last_indexed	2023-09-18T21:02:21Z
_version_	1777410707909771264

The impact of acoustic intensity on solution parameters and decellularization using sonication treatment

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