The use of sonication treatment to completely decellularize blood arteries: a pilot study

We have developed a novel sonication decellularization system to prepare completely decellularized bioscaffolds in a short treatment time. The aim of the study is to investigate the sonication decellularization efficiency and its relation with ultrasonic power output and dissolved oxygen (DO) concen...

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Main Authors:	Noor Azmi, Azran Azhim, Yamagami, K., Muramatsu, K., Morimoto, Y., Tanaka, Masato
Format:	Conference or Workshop Item
Language:	English
Published:	2011
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/44031/ http://irep.iium.edu.my/44031/ http://irep.iium.edu.my/44031/ http://irep.iium.edu.my/44031/1/paper_publish.pdf

id	iium-44031
recordtype	eprints
spelling	iium-440312015-08-06T06:23:52Z http://irep.iium.edu.my/44031/ The use of sonication treatment to completely decellularize blood arteries: a pilot study Noor Azmi, Azran Azhim Yamagami, K. Muramatsu, K. Morimoto, Y. Tanaka, Masato TP248.13 Biotechnology We have developed a novel sonication decellularization system to prepare completely decellularized bioscaffolds in a short treatment time. The aim of the study is to investigate the sonication decellularization efficiency and its relation with ultrasonic power output and dissolved oxygen (DO) concentration in different detergent solution. In the study, we used aorta samples to evaluate sonication decellularization efficiency, which assessed treatment duration, sonication power and SDS detergent with/without saline. The treated samples were evaluated histologically by Hematoxylin Eosin (HE) staining and scanning electron microscopic (SEM) photographs. The concentration of DO was monitored to identify the effect of sonication on cavitation-related DO concentration in the solution. From histological results, the sonication decellularization efficiency was better than the other preparation methods. Decellularization efficiency was tended to increase significantly when DO value decreasing after 6 hours of treatment. In conclusion, we conclude that sonication treatment can be used to prepare the complete decellularized scaffolds in short treatment time. 2011 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/44031/1/paper_publish.pdf Noor Azmi, Azran Azhim and Yamagami, K. and Muramatsu, K. and Morimoto, Y. and Tanaka, Masato (2011) The use of sonication treatment to completely decellularize blood arteries: a pilot study. In: 33rd Annual International Conference of the IEEE EMBS, 30 Aug-3 Sept. 2011, Boston, Massachusetts. http://ieeexplore.ieee.org/xpl/articleDetails.jsp?arnumber=6090685 doi:10.1109/IEMBS.2011.6090685
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Noor Azmi, Azran Azhim Yamagami, K. Muramatsu, K. Morimoto, Y. Tanaka, Masato The use of sonication treatment to completely decellularize blood arteries: a pilot study
description	We have developed a novel sonication decellularization system to prepare completely decellularized bioscaffolds in a short treatment time. The aim of the study is to investigate the sonication decellularization efficiency and its relation with ultrasonic power output and dissolved oxygen (DO) concentration in different detergent solution. In the study, we used aorta samples to evaluate sonication decellularization efficiency, which assessed treatment duration, sonication power and SDS detergent with/without saline. The treated samples were evaluated histologically by Hematoxylin Eosin (HE) staining and scanning electron microscopic (SEM) photographs. The concentration of DO was monitored to identify the effect of sonication on cavitation-related DO concentration in the solution. From histological results, the sonication decellularization efficiency was better than the other preparation methods. Decellularization efficiency was tended to increase significantly when DO value decreasing after 6 hours of treatment. In conclusion, we conclude that sonication treatment can be used to prepare the complete decellularized scaffolds in short treatment time.
format	Conference or Workshop Item
author	Noor Azmi, Azran Azhim Yamagami, K. Muramatsu, K. Morimoto, Y. Tanaka, Masato
author_facet	Noor Azmi, Azran Azhim Yamagami, K. Muramatsu, K. Morimoto, Y. Tanaka, Masato
author_sort	Noor Azmi, Azran Azhim
title	The use of sonication treatment to completely decellularize blood arteries: a pilot study
title_short	The use of sonication treatment to completely decellularize blood arteries: a pilot study
title_full	The use of sonication treatment to completely decellularize blood arteries: a pilot study
title_fullStr	The use of sonication treatment to completely decellularize blood arteries: a pilot study
title_full_unstemmed	The use of sonication treatment to completely decellularize blood arteries: a pilot study
title_sort	use of sonication treatment to completely decellularize blood arteries: a pilot study
publishDate	2011
url	http://irep.iium.edu.my/44031/ http://irep.iium.edu.my/44031/ http://irep.iium.edu.my/44031/ http://irep.iium.edu.my/44031/1/paper_publish.pdf
first_indexed	2023-09-18T21:02:38Z
last_indexed	2023-09-18T21:02:38Z
_version_	1777410725046648832

The use of sonication treatment to completely decellularize blood arteries: a pilot study

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