In vivo study of implanted aortic scaffolds decelluraized by sonication treatment

Sonication treatment is used in the preparation of scaffold that elicitsminimal immune response after implantation. The aim of this study is to investigate the immunologic responseof sonicatedly decellularizedaortic scaffoldsafter 7 and 35 days of subcutaneousimplantation. In this study, aorta sampl...

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Main Authors:	Noor Azmi, Azran Azhim, N. , Syazwani, Y. , Morimoto, K.S. , Furukawa, T. , Ushida
Format:	Conference or Workshop Item
Language:	English English
Published:	2013
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/44038/ http://irep.iium.edu.my/44038/4/proceeding_book.pdf http://irep.iium.edu.my/44038/1/IN_VIVO_STUDY_OF_IMPLANTED_AORTIC_SCAFFOLDS_DECELLULARIZED_BY.pdf

id	iium-44038
recordtype	eprints
spelling	iium-440382015-08-19T03:14:39Z http://irep.iium.edu.my/44038/ In vivo study of implanted aortic scaffolds decelluraized by sonication treatment Noor Azmi, Azran Azhim N. , Syazwani Y. , Morimoto K.S. , Furukawa T. , Ushida TP248.13 Biotechnology Sonication treatment is used in the preparation of scaffold that elicitsminimal immune response after implantation. The aim of this study is to investigate the immunologic responseof sonicatedly decellularizedaortic scaffoldsafter 7 and 35 days of subcutaneousimplantation. In this study, aorta samples weresonicatedly decellularized in0.1% and 2% SDS detergentfor 10 hours. It wasfollowed by washing process withPBS solution for 5 days. The treated samples were then evaluated histologically by Hematoxylin Eosin (HE) stainingand DNA quantification. The samples were subcutaneously implanted on the lower right thoracic cavity ofWistar rats. The implants were explanted and evaluated by HE staining. From the histological results, the implanted samples after sonicatedly decellularized with 2% SDS shows minimal immune response,compared to that decellularized with 0.1% SDS detergents. Hence, sonicatedly decellularized scaffolds treated with 2% SDS showedgreat potential as future biomedical implants. 2013 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/44038/4/proceeding_book.pdf application/pdf en http://irep.iium.edu.my/44038/1/IN_VIVO_STUDY_OF_IMPLANTED_AORTIC_SCAFFOLDS_DECELLULARIZED_BY.pdf Noor Azmi, Azran Azhim and N. , Syazwani and Y. , Morimoto and K.S. , Furukawa and T. , Ushida (2013) In vivo study of implanted aortic scaffolds decelluraized by sonication treatment. In: The MJIIT-JUC Joint International Symposium 2013 (MJJIS2013), 6-8 November 2013, Tokai University. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Noor Azmi, Azran Azhim N. , Syazwani Y. , Morimoto K.S. , Furukawa T. , Ushida In vivo study of implanted aortic scaffolds decelluraized by sonication treatment
description	Sonication treatment is used in the preparation of scaffold that elicitsminimal immune response after implantation. The aim of this study is to investigate the immunologic responseof sonicatedly decellularizedaortic scaffoldsafter 7 and 35 days of subcutaneousimplantation. In this study, aorta samples weresonicatedly decellularized in0.1% and 2% SDS detergentfor 10 hours. It wasfollowed by washing process withPBS solution for 5 days. The treated samples were then evaluated histologically by Hematoxylin Eosin (HE) stainingand DNA quantification. The samples were subcutaneously implanted on the lower right thoracic cavity ofWistar rats. The implants were explanted and evaluated by HE staining. From the histological results, the implanted samples after sonicatedly decellularized with 2% SDS shows minimal immune response,compared to that decellularized with 0.1% SDS detergents. Hence, sonicatedly decellularized scaffolds treated with 2% SDS showedgreat potential as future biomedical implants.
format	Conference or Workshop Item
author	Noor Azmi, Azran Azhim N. , Syazwani Y. , Morimoto K.S. , Furukawa T. , Ushida
author_facet	Noor Azmi, Azran Azhim N. , Syazwani Y. , Morimoto K.S. , Furukawa T. , Ushida
author_sort	Noor Azmi, Azran Azhim
title	In vivo study of implanted aortic scaffolds decelluraized by sonication treatment
title_short	In vivo study of implanted aortic scaffolds decelluraized by sonication treatment
title_full	In vivo study of implanted aortic scaffolds decelluraized by sonication treatment
title_fullStr	In vivo study of implanted aortic scaffolds decelluraized by sonication treatment
title_full_unstemmed	In vivo study of implanted aortic scaffolds decelluraized by sonication treatment
title_sort	in vivo study of implanted aortic scaffolds decelluraized by sonication treatment
publishDate	2013
url	http://irep.iium.edu.my/44038/ http://irep.iium.edu.my/44038/4/proceeding_book.pdf http://irep.iium.edu.my/44038/1/IN_VIVO_STUDY_OF_IMPLANTED_AORTIC_SCAFFOLDS_DECELLULARIZED_BY.pdf
first_indexed	2023-09-18T21:02:38Z
last_indexed	2023-09-18T21:02:38Z
_version_	1777410725754437632

In vivo study of implanted aortic scaffolds decelluraized by sonication treatment

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