Development of a module for cell culture based on a decellularized vascular tissue scaffold

Decellularized tissues and organs have been intensively studied in order to use them as a scaffold for proliferation of recipient cells in tissue engineering/regenerative medicine applications. We established a fast and effective method for tissue decellularization by way of shaking a tissue in a de...

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Main Authors:	Ataru , MURAHARA, Akimitsu , WAKIDA, Yu , MIYAKE, Shoichiro , TATEISHI, Takahiro , HIRAYAMA, Noor Azmi, Azran Azhim, Kazuaki , MURAMATSU, Masato , TANAKA, Osamu , ENDO, Kozo , MIYAZAKI, Hirotaka , MATSUO, Nariyoshi , SHINOMIYA, Yuji , MORIMOTO
Format:	Article
Language:	English English
Published:	J-STAGE 2011
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/44074/ http://irep.iium.edu.my/44074/ http://irep.iium.edu.my/44074/ http://irep.iium.edu.my/44074/1/Development_of_a_Module_for_Cell_Culture_Based_on_a_Decellularized_Vascular_Tissue_Scaffold.pdf http://irep.iium.edu.my/44074/4/Development_of_a_Module_for_Cell_Culture_Based_on_a_Decellularized_Vascular_Tissue_Scaffold.pdf

id	iium-44074
recordtype	eprints
spelling	iium-440742015-08-11T00:42:07Z http://irep.iium.edu.my/44074/ Development of a module for cell culture based on a decellularized vascular tissue scaffold Ataru , MURAHARA Akimitsu , WAKIDA Yu , MIYAKE Shoichiro , TATEISHI Takahiro , HIRAYAMA Noor Azmi, Azran Azhim Kazuaki , MURAMATSU Masato , TANAKA Osamu , ENDO Kozo , MIYAZAKI Hirotaka , MATSUO Nariyoshi , SHINOMIYA Yuji , MORIMOTO TP248.13 Biotechnology Decellularized tissues and organs have been intensively studied in order to use them as a scaffold for proliferation of recipient cells in tissue engineering/regenerative medicine applications. We established a fast and effective method for tissue decellularization by way of shaking a tissue in a detergent at 300 rpm. In addition, we developed a ring-clamp for fixation of decellularized tissue and created a scaffold module for cell culture by the combination of the ring-clamp and decellularized tissue. The module can be placed in a well of a commercially available 24-well plate. Smooth muscle cells that were seeded on a scaffold module consisting of a decellularized vessel and the ring-clamp showed proliferation with a spindle shape similar to that of vascular smooth muscle cells in the biological body. Vascular endothelial cells that adhered to the scaffold module showed enhanced susceptibility to histamine-induced Ca2+ influx. J-STAGE 2011 Article PeerReviewed application/pdf en http://irep.iium.edu.my/44074/1/Development_of_a_Module_for_Cell_Culture_Based_on_a_Decellularized_Vascular_Tissue_Scaffold.pdf application/pdf en http://irep.iium.edu.my/44074/4/Development_of_a_Module_for_Cell_Culture_Based_on_a_Decellularized_Vascular_Tissue_Scaffold.pdf Ataru , MURAHARA and Akimitsu , WAKIDA and Yu , MIYAKE and Shoichiro , TATEISHI and Takahiro , HIRAYAMA and Noor Azmi, Azran Azhim and Kazuaki , MURAMATSU and Masato , TANAKA and Osamu , ENDO and Kozo , MIYAZAKI and Hirotaka , MATSUO and Nariyoshi , SHINOMIYA and Yuji , MORIMOTO (2011) Development of a module for cell culture based on a decellularized vascular tissue scaffold. Transactions of Japanese Society for Medical and Biological Engineering , 49 (3). pp. 508-515. ISSN 1881-4379 https://www.jstage.jst.go.jp/article/jsmbe/49/3/49_3_508/_article http://doi.org/10.11239/jsmbe.49.508
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Ataru , MURAHARA Akimitsu , WAKIDA Yu , MIYAKE Shoichiro , TATEISHI Takahiro , HIRAYAMA Noor Azmi, Azran Azhim Kazuaki , MURAMATSU Masato , TANAKA Osamu , ENDO Kozo , MIYAZAKI Hirotaka , MATSUO Nariyoshi , SHINOMIYA Yuji , MORIMOTO Development of a module for cell culture based on a decellularized vascular tissue scaffold
description	Decellularized tissues and organs have been intensively studied in order to use them as a scaffold for proliferation of recipient cells in tissue engineering/regenerative medicine applications. We established a fast and effective method for tissue decellularization by way of shaking a tissue in a detergent at 300 rpm. In addition, we developed a ring-clamp for fixation of decellularized tissue and created a scaffold module for cell culture by the combination of the ring-clamp and decellularized tissue. The module can be placed in a well of a commercially available 24-well plate. Smooth muscle cells that were seeded on a scaffold module consisting of a decellularized vessel and the ring-clamp showed proliferation with a spindle shape similar to that of vascular smooth muscle cells in the biological body. Vascular endothelial cells that adhered to the scaffold module showed enhanced susceptibility to histamine-induced Ca2+ influx.
format	Article
author	Ataru , MURAHARA Akimitsu , WAKIDA Yu , MIYAKE Shoichiro , TATEISHI Takahiro , HIRAYAMA Noor Azmi, Azran Azhim Kazuaki , MURAMATSU Masato , TANAKA Osamu , ENDO Kozo , MIYAZAKI Hirotaka , MATSUO Nariyoshi , SHINOMIYA Yuji , MORIMOTO
author_facet	Ataru , MURAHARA Akimitsu , WAKIDA Yu , MIYAKE Shoichiro , TATEISHI Takahiro , HIRAYAMA Noor Azmi, Azran Azhim Kazuaki , MURAMATSU Masato , TANAKA Osamu , ENDO Kozo , MIYAZAKI Hirotaka , MATSUO Nariyoshi , SHINOMIYA Yuji , MORIMOTO
author_sort	Ataru , MURAHARA
title	Development of a module for cell culture based on a decellularized vascular tissue scaffold
title_short	Development of a module for cell culture based on a decellularized vascular tissue scaffold
title_full	Development of a module for cell culture based on a decellularized vascular tissue scaffold
title_fullStr	Development of a module for cell culture based on a decellularized vascular tissue scaffold
title_full_unstemmed	Development of a module for cell culture based on a decellularized vascular tissue scaffold
title_sort	development of a module for cell culture based on a decellularized vascular tissue scaffold
publisher	J-STAGE
publishDate	2011
url	http://irep.iium.edu.my/44074/ http://irep.iium.edu.my/44074/ http://irep.iium.edu.my/44074/ http://irep.iium.edu.my/44074/1/Development_of_a_Module_for_Cell_Culture_Based_on_a_Decellularized_Vascular_Tissue_Scaffold.pdf http://irep.iium.edu.my/44074/4/Development_of_a_Module_for_Cell_Culture_Based_on_a_Decellularized_Vascular_Tissue_Scaffold.pdf
first_indexed	2023-09-18T21:02:42Z
last_indexed	2023-09-18T21:02:42Z
_version_	1777410729390899200

Development of a module for cell culture based on a decellularized vascular tissue scaffold

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