Decellularization of meniscal tissue using ultrasound chemical process for tissue-engineered scaffold applications

Scaffolds play a key role in the process of regeneration and morphogenesis of tissue or organ. We have studied a novel application of ultrasound irradiation to prepare decellularized tissue for tissue-engineered scaffolds. The aim of the present study is to investigate the possibility of ultrasound...

Full description

Bibliographic Details
Main Authors:	Noor Azmi, Azran Azhim, Takahashi, T., Muramatsu, K., Morimoto, Y., Tanaka, Takanori
Format:	Conference or Workshop Item
Language:	English English
Published:	Springer Berlin Heidelberg 2010
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/44077/ http://irep.iium.edu.my/44077/ http://irep.iium.edu.my/44077/ http://irep.iium.edu.my/44077/1/Decellularization_of_Meniscal_Tissue_Using_Ultrasound_Chemical_Process_for_tissue_engineered_scaffold_application.pdf http://irep.iium.edu.my/44077/4/44077.pdf

id	iium-44077
recordtype	eprints
spelling	iium-440772015-08-04T07:33:13Z http://irep.iium.edu.my/44077/ Decellularization of meniscal tissue using ultrasound chemical process for tissue-engineered scaffold applications Noor Azmi, Azran Azhim Takahashi, T. Muramatsu, K. Morimoto, Y. Tanaka, Takanori TP248.13 Biotechnology Scaffolds play a key role in the process of regeneration and morphogenesis of tissue or organ. We have studied a novel application of ultrasound irradiation to prepare decellularized tissue for tissue-engineered scaffolds. The aim of the present study is to investigate the possibility of ultrasound energy on decellularization of cartilage tissue. The samples were decellularized using 20 kHz of ultrasonic irradiation in circulated 0.3% sodium chloride (NaCl) constituent of 2% sodium dodecyl sulfate (SDS) solution. To evaluate tissue integrity and cell removal, we used hematoxylin-eosin staining. To estimate the degree of irradiated ultrasound on the sample, we used computer simulation to calculate the irradiated power distribution in solution, corresponding to the irradiation energy against to the sample. From histological results, it shows that the ultrasonic power could assist the decellularization on meniscal tissue. The decellularization efficiency was better than the other preparation methods. Because of distributions of ultrasonic irradiation to samples were non-uniform, the efficiency of cell removal could be improved by using 3 dimensional scanning system which uniformly irradiate to the sample. In conclusion, ultrasonic chemical process has a potential to improve the efficiency of decellularization for menisci. Springer Berlin Heidelberg 2010 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/44077/1/Decellularization_of_Meniscal_Tissue_Using_Ultrasound_Chemical_Process_for_tissue_engineered_scaffold_application.pdf application/pdf en http://irep.iium.edu.my/44077/4/44077.pdf Noor Azmi, Azran Azhim and Takahashi, T. and Muramatsu, K. and Morimoto, Y. and Tanaka, Takanori (2010) Decellularization of meniscal tissue using ultrasound chemical process for tissue-engineered scaffold applications. In: 6th World Congress of Biomechanics (WCB 2010), 1-6 August 2010, Singapore. http://link.springer.com/chapter/10.1007%2F978-3-642-14515-5_233 doi:10.1007/978-3-642-14515-5_233
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Noor Azmi, Azran Azhim Takahashi, T. Muramatsu, K. Morimoto, Y. Tanaka, Takanori Decellularization of meniscal tissue using ultrasound chemical process for tissue-engineered scaffold applications
description	Scaffolds play a key role in the process of regeneration and morphogenesis of tissue or organ. We have studied a novel application of ultrasound irradiation to prepare decellularized tissue for tissue-engineered scaffolds. The aim of the present study is to investigate the possibility of ultrasound energy on decellularization of cartilage tissue. The samples were decellularized using 20 kHz of ultrasonic irradiation in circulated 0.3% sodium chloride (NaCl) constituent of 2% sodium dodecyl sulfate (SDS) solution. To evaluate tissue integrity and cell removal, we used hematoxylin-eosin staining. To estimate the degree of irradiated ultrasound on the sample, we used computer simulation to calculate the irradiated power distribution in solution, corresponding to the irradiation energy against to the sample. From histological results, it shows that the ultrasonic power could assist the decellularization on meniscal tissue. The decellularization efficiency was better than the other preparation methods. Because of distributions of ultrasonic irradiation to samples were non-uniform, the efficiency of cell removal could be improved by using 3 dimensional scanning system which uniformly irradiate to the sample. In conclusion, ultrasonic chemical process has a potential to improve the efficiency of decellularization for menisci.
format	Conference or Workshop Item
author	Noor Azmi, Azran Azhim Takahashi, T. Muramatsu, K. Morimoto, Y. Tanaka, Takanori
author_facet	Noor Azmi, Azran Azhim Takahashi, T. Muramatsu, K. Morimoto, Y. Tanaka, Takanori
author_sort	Noor Azmi, Azran Azhim
title	Decellularization of meniscal tissue using ultrasound chemical process for tissue-engineered scaffold applications
title_short	Decellularization of meniscal tissue using ultrasound chemical process for tissue-engineered scaffold applications
title_full	Decellularization of meniscal tissue using ultrasound chemical process for tissue-engineered scaffold applications
title_fullStr	Decellularization of meniscal tissue using ultrasound chemical process for tissue-engineered scaffold applications
title_full_unstemmed	Decellularization of meniscal tissue using ultrasound chemical process for tissue-engineered scaffold applications
title_sort	decellularization of meniscal tissue using ultrasound chemical process for tissue-engineered scaffold applications
publisher	Springer Berlin Heidelberg
publishDate	2010
url	http://irep.iium.edu.my/44077/ http://irep.iium.edu.my/44077/ http://irep.iium.edu.my/44077/ http://irep.iium.edu.my/44077/1/Decellularization_of_Meniscal_Tissue_Using_Ultrasound_Chemical_Process_for_tissue_engineered_scaffold_application.pdf http://irep.iium.edu.my/44077/4/44077.pdf
first_indexed	2023-09-18T21:02:42Z
last_indexed	2023-09-18T21:02:42Z
_version_	1777410729679257600

Decellularization of meniscal tissue using ultrasound chemical process for tissue-engineered scaffold applications

Similar Items