Screening cellulolytic fungi isolated from Malaysia cocoa pod husk and its culture conditions for cellulases production

The aim of this study was to screen few fungal isolates from local cocoa pod husks (CPH) which able to secrete cellulases. The isolates were plated on carboxymethyl cellulose (CMC) agar plates which then incubated for two days at 28ºC. Then, these plates were stained with congo red dye for 0.5-1 h...

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Main Authors:	Jimat, Dzun Noraini, Azmi, Azlin Suhaida, Ahmad Raus, Raha, Talha, Nur Syakirah
Format:	Article
Language:	English
Published:	Faculty of Agro based Industry (FIAT), Universiti Malaysia Kelantan (UMK) 2015
Subjects:	TP500 Fermentation industries. Beverages. Alcohol
Online Access:	http://irep.iium.edu.my/44096/ http://irep.iium.edu.my/44096/ http://irep.iium.edu.my/44096/1/3-1-185-190_ctress_2014.pdf

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recordtype	eprints
spelling	iium-440962017-11-23T08:41:16Z http://irep.iium.edu.my/44096/ Screening cellulolytic fungi isolated from Malaysia cocoa pod husk and its culture conditions for cellulases production Jimat, Dzun Noraini Azmi, Azlin Suhaida Ahmad Raus, Raha Talha, Nur Syakirah TP500 Fermentation industries. Beverages. Alcohol The aim of this study was to screen few fungal isolates from local cocoa pod husks (CPH) which able to secrete cellulases. The isolates were plated on carboxymethyl cellulose (CMC) agar plates which then incubated for two days at 28ºC. Then, these plates were stained with congo red dye for 0.5-1 h followed by destaining with 1 M NaCl solution for 15-20 minutes to observe its cellulolytic activity. One isolates which exposed the largest cellulolytic zone on CMC agar plate was selected for further study. In this study, culture conditions with respect to pH, incubation time, amount of substrate (CPH) and temperature were screened using Design expert version 8.0 by employing two-level factorial design. The selected fungus isolate was cultured in shake flask at 37°C with agitation of 200 rpm for 5 days in incubator shaker. During fermentation period, samples were collected every day for fungal-cellulases activity of filter paper activity (FPase) and carboxymethyl cellulase (CMCase) activity. Analysis of variance (ANOVA) of this study showed that the most significant parameters that affects the production of cellulases from the selected fungi isolates were the amount of substrate (CPH) used followed by the interaction of amount of substrate with pH (p< 0.05). It showed that the cellulases activity was high when the pH 9 with more amount of substrate used. However, it was observed that less significant changes of celllulases activity when different amount of substrate was used at same pH of 3. Based on the microscopic observation of isolate, its morphology was closed to Rhizopus sp.. Further study is essential to optimize the selected culture parameters obtained in this study in order to maximize the activity of cellulases from the selected isolates. Faculty of Agro based Industry (FIAT), Universiti Malaysia Kelantan (UMK) 2015 Article NonPeerReviewed application/pdf en http://irep.iium.edu.my/44096/1/3-1-185-190_ctress_2014.pdf Jimat, Dzun Noraini and Azmi, Azlin Suhaida and Ahmad Raus, Raha and Talha, Nur Syakirah (2015) Screening cellulolytic fungi isolated from Malaysia cocoa pod husk and its culture conditions for cellulases production. Journal of Tropical Resources and Sustainable Science (JTRSS), 3. pp. 185-190. ISSN 2289-3946 http://www.jtrss.org/JTRSS/volume3/UN-44/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP500 Fermentation industries. Beverages. Alcohol
spellingShingle	TP500 Fermentation industries. Beverages. Alcohol Jimat, Dzun Noraini Azmi, Azlin Suhaida Ahmad Raus, Raha Talha, Nur Syakirah Screening cellulolytic fungi isolated from Malaysia cocoa pod husk and its culture conditions for cellulases production
description	The aim of this study was to screen few fungal isolates from local cocoa pod husks (CPH) which able to secrete cellulases. The isolates were plated on carboxymethyl cellulose (CMC) agar plates which then incubated for two days at 28ºC. Then, these plates were stained with congo red dye for 0.5-1 h followed by destaining with 1 M NaCl solution for 15-20 minutes to observe its cellulolytic activity. One isolates which exposed the largest cellulolytic zone on CMC agar plate was selected for further study. In this study, culture conditions with respect to pH, incubation time, amount of substrate (CPH) and temperature were screened using Design expert version 8.0 by employing two-level factorial design. The selected fungus isolate was cultured in shake flask at 37°C with agitation of 200 rpm for 5 days in incubator shaker. During fermentation period, samples were collected every day for fungal-cellulases activity of filter paper activity (FPase) and carboxymethyl cellulase (CMCase) activity. Analysis of variance (ANOVA) of this study showed that the most significant parameters that affects the production of cellulases from the selected fungi isolates were the amount of substrate (CPH) used followed by the interaction of amount of substrate with pH (p< 0.05). It showed that the cellulases activity was high when the pH 9 with more amount of substrate used. However, it was observed that less significant changes of celllulases activity when different amount of substrate was used at same pH of 3. Based on the microscopic observation of isolate, its morphology was closed to Rhizopus sp.. Further study is essential to optimize the selected culture parameters obtained in this study in order to maximize the activity of cellulases from the selected isolates.
format	Article
author	Jimat, Dzun Noraini Azmi, Azlin Suhaida Ahmad Raus, Raha Talha, Nur Syakirah
author_facet	Jimat, Dzun Noraini Azmi, Azlin Suhaida Ahmad Raus, Raha Talha, Nur Syakirah
author_sort	Jimat, Dzun Noraini
title	Screening cellulolytic fungi isolated from Malaysia cocoa pod husk and its culture conditions for cellulases production
title_short	Screening cellulolytic fungi isolated from Malaysia cocoa pod husk and its culture conditions for cellulases production
title_full	Screening cellulolytic fungi isolated from Malaysia cocoa pod husk and its culture conditions for cellulases production
title_fullStr	Screening cellulolytic fungi isolated from Malaysia cocoa pod husk and its culture conditions for cellulases production
title_full_unstemmed	Screening cellulolytic fungi isolated from Malaysia cocoa pod husk and its culture conditions for cellulases production
title_sort	screening cellulolytic fungi isolated from malaysia cocoa pod husk and its culture conditions for cellulases production
publisher	Faculty of Agro based Industry (FIAT), Universiti Malaysia Kelantan (UMK)
publishDate	2015
url	http://irep.iium.edu.my/44096/ http://irep.iium.edu.my/44096/ http://irep.iium.edu.my/44096/1/3-1-185-190_ctress_2014.pdf
first_indexed	2023-09-18T21:02:44Z
last_indexed	2023-09-18T21:02:44Z
_version_	1777410731375853568

Screening cellulolytic fungi isolated from Malaysia cocoa pod husk and its culture conditions for cellulases production

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