Photodynamic therapy mediates innate immune responses via fibroblast-macrophage interactions

Antibacterial photodynamic therapy (PDT) has come to attract attention as an alternative therapy for drug-resistant bacteria. Recent reports revealed that antibacterial PDT induces innate immune response and stimulates abundant cytokine secretion as a part of inflammatory responses. However, the und...

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Main Authors:	Zulaziz, Natasha, Azhim, Azran, Himeno, Naomi, Tanaka, Masato, Satoh, Yasushi, Kinoshita, Manabu, Miyazaki, Hiromi, Saitoh, Daizoh, Shinomiya, Nariyoshi, Morimoto, Y.
Format:	Article
Language:	English
Published:	Springer Japan 2015
Subjects:	RZ Other systems of medicine TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/44151/ http://irep.iium.edu.my/44151/ http://irep.iium.edu.my/44151/ http://irep.iium.edu.my/44151/1/Photodynamic_therapy_mediates_innate_immune_responses.pdf

id	iium-44151
recordtype	eprints
spelling	iium-441512015-09-14T01:35:09Z http://irep.iium.edu.my/44151/ Photodynamic therapy mediates innate immune responses via fibroblast-macrophage interactions Zulaziz, Natasha Azhim, Azran Himeno, Naomi Tanaka, Masato Satoh, Yasushi Kinoshita, Manabu Miyazaki, Hiromi Saitoh, Daizoh Shinomiya, Nariyoshi Morimoto, Y. RZ Other systems of medicine TP248.13 Biotechnology Antibacterial photodynamic therapy (PDT) has come to attract attention as an alternative therapy for drug-resistant bacteria. Recent reports revealed that antibacterial PDT induces innate immune response and stimulates abundant cytokine secretion as a part of inflammatory responses. However, the underlying mechanism how antibacterial PDT interacts with immune cells responsible for cytokine secretion has not been well outlined. In this study, we aimed to clarify the difference in gene expression and cytokine secretion between combined culture of fibroblasts and macrophages and their independent cultures. SCRC-1008, mouse fibroblast cell line and J774, mouse macrophage-like cell line were co-cultured and PDT treatments with different parameters were carried out. After various incubation periods (1-24 h), cells and culture medium were collected, and mRNA and protein levels for cytokines were measured using real-time PCR and ELISA, respectively. Our results showed that fibroblasts and macrophages interact with each other to mediate the immune response. We propose that fibroblasts initially respond to PDT by expressing Hspa1b, which regulates the NF-κB pathway via Tlr2 and Tlr4. Activation of the NF-κB pathway then results in an enhanced secretion of pro-inflammatory cytokines (TNF-α, IL-6 and IL-1β) and neutrophil chemoattractant MIP-2 and KC from macrophages. Springer Japan 2015-05-22 Article PeerReviewed application/pdf en http://irep.iium.edu.my/44151/1/Photodynamic_therapy_mediates_innate_immune_responses.pdf Zulaziz, Natasha and Azhim, Azran and Himeno, Naomi and Tanaka, Masato and Satoh, Yasushi and Kinoshita, Manabu and Miyazaki, Hiromi and Saitoh, Daizoh and Shinomiya, Nariyoshi and Morimoto, Y. (2015) Photodynamic therapy mediates innate immune responses via fibroblast-macrophage interactions. Human Cell. ISSN 1749-0774 http://link.springer.com/article/10.1007%2Fs13577-015-0118-2 10.1007/s13577-015-0118-2
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RZ Other systems of medicine TP248.13 Biotechnology
spellingShingle	RZ Other systems of medicine TP248.13 Biotechnology Zulaziz, Natasha Azhim, Azran Himeno, Naomi Tanaka, Masato Satoh, Yasushi Kinoshita, Manabu Miyazaki, Hiromi Saitoh, Daizoh Shinomiya, Nariyoshi Morimoto, Y. Photodynamic therapy mediates innate immune responses via fibroblast-macrophage interactions
description	Antibacterial photodynamic therapy (PDT) has come to attract attention as an alternative therapy for drug-resistant bacteria. Recent reports revealed that antibacterial PDT induces innate immune response and stimulates abundant cytokine secretion as a part of inflammatory responses. However, the underlying mechanism how antibacterial PDT interacts with immune cells responsible for cytokine secretion has not been well outlined. In this study, we aimed to clarify the difference in gene expression and cytokine secretion between combined culture of fibroblasts and macrophages and their independent cultures. SCRC-1008, mouse fibroblast cell line and J774, mouse macrophage-like cell line were co-cultured and PDT treatments with different parameters were carried out. After various incubation periods (1-24 h), cells and culture medium were collected, and mRNA and protein levels for cytokines were measured using real-time PCR and ELISA, respectively. Our results showed that fibroblasts and macrophages interact with each other to mediate the immune response. We propose that fibroblasts initially respond to PDT by expressing Hspa1b, which regulates the NF-κB pathway via Tlr2 and Tlr4. Activation of the NF-κB pathway then results in an enhanced secretion of pro-inflammatory cytokines (TNF-α, IL-6 and IL-1β) and neutrophil chemoattractant MIP-2 and KC from macrophages.
format	Article
author	Zulaziz, Natasha Azhim, Azran Himeno, Naomi Tanaka, Masato Satoh, Yasushi Kinoshita, Manabu Miyazaki, Hiromi Saitoh, Daizoh Shinomiya, Nariyoshi Morimoto, Y.
author_facet	Zulaziz, Natasha Azhim, Azran Himeno, Naomi Tanaka, Masato Satoh, Yasushi Kinoshita, Manabu Miyazaki, Hiromi Saitoh, Daizoh Shinomiya, Nariyoshi Morimoto, Y.
author_sort	Zulaziz, Natasha
title	Photodynamic therapy mediates innate immune responses via fibroblast-macrophage interactions
title_short	Photodynamic therapy mediates innate immune responses via fibroblast-macrophage interactions
title_full	Photodynamic therapy mediates innate immune responses via fibroblast-macrophage interactions
title_fullStr	Photodynamic therapy mediates innate immune responses via fibroblast-macrophage interactions
title_full_unstemmed	Photodynamic therapy mediates innate immune responses via fibroblast-macrophage interactions
title_sort	photodynamic therapy mediates innate immune responses via fibroblast-macrophage interactions
publisher	Springer Japan
publishDate	2015
url	http://irep.iium.edu.my/44151/ http://irep.iium.edu.my/44151/ http://irep.iium.edu.my/44151/ http://irep.iium.edu.my/44151/1/Photodynamic_therapy_mediates_innate_immune_responses.pdf
first_indexed	2023-09-18T21:02:48Z
last_indexed	2023-09-18T21:02:48Z
_version_	1777410735763095552

Photodynamic therapy mediates innate immune responses via fibroblast-macrophage interactions

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