A novel animal model for subcutaneous soft tissue infection

In order to simultaneously monitor neutrophil migration in vivo during inflammation, we developed a novel animal model for subcutaneous soft tissue infection using transgenic mouse bearing fluorescence-positive neutrophils and bioluminescent methicillin-resistant Staphylococcus aureus (MRSA). Metho...

Full description

Bibliographic Details
Main Authors:	Zulaziz, Natasha, N. , Himeno, H. , Miyazaki, D. , Saitoh, Noor Azmi, Azran Azhim, Y. , Morimoto
Format:	Article
Language:	English
Published:	J-STAGE 2014
Subjects:	RZ Other systems of medicine TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/44152/ http://irep.iium.edu.my/44152/ http://irep.iium.edu.my/44152/ http://irep.iium.edu.my/44152/1/A_Novel_Animal_Model_for_Subcutaneous_Soft_Tissue_Infection.pdf

id	iium-44152
recordtype	eprints
spelling	iium-441522015-08-18T00:20:18Z http://irep.iium.edu.my/44152/ A novel animal model for subcutaneous soft tissue infection Zulaziz, Natasha N. , Himeno H. , Miyazaki D. , Saitoh Noor Azmi, Azran Azhim Y. , Morimoto RZ Other systems of medicine TP248.13 Biotechnology In order to simultaneously monitor neutrophil migration in vivo during inflammation, we developed a novel animal model for subcutaneous soft tissue infection using transgenic mouse bearing fluorescence-positive neutrophils and bioluminescent methicillin-resistant Staphylococcus aureus (MRSA). Methods: Eight to nine-week-old male lys-EGFP C57BL/6 were anesthetized (50 mg/kg pentobarbital sodium, ip) and 1.0 ×107 CFU of bioluminescent MRSA (Xen31, PerkinElmer) were subcutaneously injected. Control group was injected with PBS while the treatment group was given Arbekacin (25 mg/kg) intravenously via tail vein. For the evaluation of MRSA activity and neutrophil accumulation, an in vivo imaging system (LAS-4000, GE) was performed. Results: Both groups displayed similar pattern with significant drop in MRSA bioluminescence and neutrophil fluorescence peaked on day 1. However, bacterial infection completely resolved in treatment group by day 6 with gradual decline in neutrophil fluorescence. Conclusions: This animal model could be a competent method for assessment of subcutaneous soft tissue infections. J-STAGE 2014 Article PeerReviewed application/pdf en http://irep.iium.edu.my/44152/1/A_Novel_Animal_Model_for_Subcutaneous_Soft_Tissue_Infection.pdf Zulaziz, Natasha and N. , Himeno and H. , Miyazaki and D. , Saitoh and Noor Azmi, Azran Azhim and Y. , Morimoto (2014) A novel animal model for subcutaneous soft tissue infection. Biomedical Engineering, 52. pp. 285-286. ISSN 1881-4379 https://www.jstage.jst.go.jp/article/jsmbe/52/Supplement/52_O-285/_article http://doi.org/10.11239/jsmbe.52.O-285
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RZ Other systems of medicine TP248.13 Biotechnology
spellingShingle	RZ Other systems of medicine TP248.13 Biotechnology Zulaziz, Natasha N. , Himeno H. , Miyazaki D. , Saitoh Noor Azmi, Azran Azhim Y. , Morimoto A novel animal model for subcutaneous soft tissue infection
description	In order to simultaneously monitor neutrophil migration in vivo during inflammation, we developed a novel animal model for subcutaneous soft tissue infection using transgenic mouse bearing fluorescence-positive neutrophils and bioluminescent methicillin-resistant Staphylococcus aureus (MRSA). Methods: Eight to nine-week-old male lys-EGFP C57BL/6 were anesthetized (50 mg/kg pentobarbital sodium, ip) and 1.0 ×107 CFU of bioluminescent MRSA (Xen31, PerkinElmer) were subcutaneously injected. Control group was injected with PBS while the treatment group was given Arbekacin (25 mg/kg) intravenously via tail vein. For the evaluation of MRSA activity and neutrophil accumulation, an in vivo imaging system (LAS-4000, GE) was performed. Results: Both groups displayed similar pattern with significant drop in MRSA bioluminescence and neutrophil fluorescence peaked on day 1. However, bacterial infection completely resolved in treatment group by day 6 with gradual decline in neutrophil fluorescence. Conclusions: This animal model could be a competent method for assessment of subcutaneous soft tissue infections.
format	Article
author	Zulaziz, Natasha N. , Himeno H. , Miyazaki D. , Saitoh Noor Azmi, Azran Azhim Y. , Morimoto
author_facet	Zulaziz, Natasha N. , Himeno H. , Miyazaki D. , Saitoh Noor Azmi, Azran Azhim Y. , Morimoto
author_sort	Zulaziz, Natasha
title	A novel animal model for subcutaneous soft tissue infection
title_short	A novel animal model for subcutaneous soft tissue infection
title_full	A novel animal model for subcutaneous soft tissue infection
title_fullStr	A novel animal model for subcutaneous soft tissue infection
title_full_unstemmed	A novel animal model for subcutaneous soft tissue infection
title_sort	novel animal model for subcutaneous soft tissue infection
publisher	J-STAGE
publishDate	2014
url	http://irep.iium.edu.my/44152/ http://irep.iium.edu.my/44152/ http://irep.iium.edu.my/44152/ http://irep.iium.edu.my/44152/1/A_Novel_Animal_Model_for_Subcutaneous_Soft_Tissue_Infection.pdf
first_indexed	2023-09-18T21:02:48Z
last_indexed	2023-09-18T21:02:48Z
_version_	1777410735909896192

A novel animal model for subcutaneous soft tissue infection

Similar Items