Selective isolation of Actinomycetes from mangrove sediment of Tanjung Lumpur, Kuantan, Malaysia

Aims: Mangroves of Tanjung Lumpur, Kuantan, Pahang is considered as a relatively underexplored resource of actinomycetes. Based on the above perspective, a study was conducted on mangrove sediments of Tanjung Lumpur, Kuantan to isolate potential actinomycetes using several pretreatments and variou...

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Main Authors:	Mohamad, Nur Hanisah, Khan Chowdhury, Ahmed Jalal, Zainal Abidin, Zaima Azira
Format:	Article
Language:	English
Published:	Malaysian Society for Microbiology 2015
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/44691/ http://irep.iium.edu.my/44691/ http://irep.iium.edu.my/44691/1/Selective_isolation_of_Actinomycetes_from_mangrove_sediment_of_Tanjung_Lumpur%2C.pdf

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spelling	iium-446912017-12-06T05:09:54Z http://irep.iium.edu.my/44691/ Selective isolation of Actinomycetes from mangrove sediment of Tanjung Lumpur, Kuantan, Malaysia Mohamad, Nur Hanisah Khan Chowdhury, Ahmed Jalal Zainal Abidin, Zaima Azira QR Microbiology Aims: Mangroves of Tanjung Lumpur, Kuantan, Pahang is considered as a relatively underexplored resource of actinomycetes. Based on the above perspective, a study was conducted on mangrove sediments of Tanjung Lumpur, Kuantan to isolate potential actinomycetes using several pretreatments and various selective media. Methodology and results: Sediments from five different sites at Tanjung Lumpur mangrove were collected and selectively pre-treated. The pretreated sediments were diluted and plated onto eight different selective media. A total of 172 potential actinomycetes were isolated from all the media. Antimicrobial activities of 61 selected strains were checked against 8 test microorganisms using cross streak method. Pretreatment of wet heat with seawater was the most effective method for the isolation of actinomycetes as it yielded a maximum of 105 actinomycete isolates and IM7 was the most suitable medium for actinomycete isolation with highest percentage of recovery (31 %). Forty three isolates (70.5 %) showed antimicrobial activities against one or more test microorganisms. Isolates IIUM B21 and IIUM B31 showed antimicrobial activity against all test microorganisms. Seven isolates showed antifungal activity as they inhibited only C. albicans. Ten isolates were randomly selected for identification based on partial sequences of 16S rRNA gene. Six isolates were found belong to the genus Streptomyces, two isolates belong to the genus Micromonospora and two isolates were identified as Rhodococcus spp. Conclusion, significance and impact of study: These findings revealed the potential of mangrove sediment of Tanjung Lumpur as an important source of actinomycetes with biosynthetic capabilities which might be beneficial to pharmaceutical industries. Malaysian Society for Microbiology 2015 Article PeerReviewed application/pdf en http://irep.iium.edu.my/44691/1/Selective_isolation_of_Actinomycetes_from_mangrove_sediment_of_Tanjung_Lumpur%2C.pdf Mohamad, Nur Hanisah and Khan Chowdhury, Ahmed Jalal and Zainal Abidin, Zaima Azira (2015) Selective isolation of Actinomycetes from mangrove sediment of Tanjung Lumpur, Kuantan, Malaysia. Malaysian Journal of Microbiology, 11 (2). pp. 144-155. ISSN 1823-8262 (P), 2231-7538 (O) http://mjm.usm.my/uploads/issues/427/13.%20Corrected%20proof%20MJM%20126-14S.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Mohamad, Nur Hanisah Khan Chowdhury, Ahmed Jalal Zainal Abidin, Zaima Azira Selective isolation of Actinomycetes from mangrove sediment of Tanjung Lumpur, Kuantan, Malaysia
description	Aims: Mangroves of Tanjung Lumpur, Kuantan, Pahang is considered as a relatively underexplored resource of actinomycetes. Based on the above perspective, a study was conducted on mangrove sediments of Tanjung Lumpur, Kuantan to isolate potential actinomycetes using several pretreatments and various selective media. Methodology and results: Sediments from five different sites at Tanjung Lumpur mangrove were collected and selectively pre-treated. The pretreated sediments were diluted and plated onto eight different selective media. A total of 172 potential actinomycetes were isolated from all the media. Antimicrobial activities of 61 selected strains were checked against 8 test microorganisms using cross streak method. Pretreatment of wet heat with seawater was the most effective method for the isolation of actinomycetes as it yielded a maximum of 105 actinomycete isolates and IM7 was the most suitable medium for actinomycete isolation with highest percentage of recovery (31 %). Forty three isolates (70.5 %) showed antimicrobial activities against one or more test microorganisms. Isolates IIUM B21 and IIUM B31 showed antimicrobial activity against all test microorganisms. Seven isolates showed antifungal activity as they inhibited only C. albicans. Ten isolates were randomly selected for identification based on partial sequences of 16S rRNA gene. Six isolates were found belong to the genus Streptomyces, two isolates belong to the genus Micromonospora and two isolates were identified as Rhodococcus spp. Conclusion, significance and impact of study: These findings revealed the potential of mangrove sediment of Tanjung Lumpur as an important source of actinomycetes with biosynthetic capabilities which might be beneficial to pharmaceutical industries.
format	Article
author	Mohamad, Nur Hanisah Khan Chowdhury, Ahmed Jalal Zainal Abidin, Zaima Azira
author_facet	Mohamad, Nur Hanisah Khan Chowdhury, Ahmed Jalal Zainal Abidin, Zaima Azira
author_sort	Mohamad, Nur Hanisah
title	Selective isolation of Actinomycetes from mangrove sediment of Tanjung Lumpur, Kuantan, Malaysia
title_short	Selective isolation of Actinomycetes from mangrove sediment of Tanjung Lumpur, Kuantan, Malaysia
title_full	Selective isolation of Actinomycetes from mangrove sediment of Tanjung Lumpur, Kuantan, Malaysia
title_fullStr	Selective isolation of Actinomycetes from mangrove sediment of Tanjung Lumpur, Kuantan, Malaysia
title_full_unstemmed	Selective isolation of Actinomycetes from mangrove sediment of Tanjung Lumpur, Kuantan, Malaysia
title_sort	selective isolation of actinomycetes from mangrove sediment of tanjung lumpur, kuantan, malaysia
publisher	Malaysian Society for Microbiology
publishDate	2015
url	http://irep.iium.edu.my/44691/ http://irep.iium.edu.my/44691/ http://irep.iium.edu.my/44691/1/Selective_isolation_of_Actinomycetes_from_mangrove_sediment_of_Tanjung_Lumpur%2C.pdf
first_indexed	2023-09-18T21:03:32Z
last_indexed	2023-09-18T21:03:32Z
_version_	1777410781716938752

Selective isolation of Actinomycetes from mangrove sediment of Tanjung Lumpur, Kuantan, Malaysia

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