In vitro screening of Andrographis paniculata (Hempedu bumi) for antibacterial activity using disc diffusion method

The increasing prevalence in multidrug resistant strains of bacteria and the recent appearance of strains with reduced susceptibility to antibiotics raises the specter of untreatable bacterial infections and adds urgency to the search for new infection-fighting strategies1. The plant Andrographis pa...

Full description

Bibliographic Details
Main Authors:	Sule, Abubakar, Abd. Samah, Othman, Omar, Muhammad Nor, Ahmed, Qamar Uddin
Format:	Conference or Workshop Item
Language:	English
Published:	2009
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/4479/ http://irep.iium.edu.my/4479/ http://irep.iium.edu.my/4479/1/3.pdf

id	iium-4479
recordtype	eprints
spelling	iium-44792013-07-17T07:10:42Z http://irep.iium.edu.my/4479/ In vitro screening of Andrographis paniculata (Hempedu bumi) for antibacterial activity using disc diffusion method Sule, Abubakar Abd. Samah, Othman Omar, Muhammad Nor Ahmed, Qamar Uddin QD Chemistry The increasing prevalence in multidrug resistant strains of bacteria and the recent appearance of strains with reduced susceptibility to antibiotics raises the specter of untreatable bacterial infections and adds urgency to the search for new infection-fighting strategies1. The plant Andrographis paniculata (Hempedu Bumi) is used in traditional medicinal practice for the treatment of various chronic and infectious diseases some of bacterial origin2.. This study was carried out to evaluate the antibacterial activity of organic extracts of A. paniculata leaves and stems against 8 pathogenic bacterial strains: Staphylococcus aureus, Streptococcus pyogenes, Bacillus anthrax Micrococcus luteus, Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas aeroginosa and Proteus mirabilis using disc diffusion method. Dried ground powdered leaves and stems were extracted with petroleum ether, dichloromethane, ethyl acetate, acetone, ethanol and finally with methanol using the soxhlet extraction. The result obtained vividly revealed that the different extracts differed significantly in their antibacterial properties with Dichloromethane extract being the most effective followed by acetone, ethyl acetate, methanol, petroleum ether and lastly ethanol extract. In general, the leaves exhibited strong antibacterial activity than the stems extracts; but all the extracts exerted very good broad spectrum activity against all tested microorganisms. The results explicitly suggest that there might be polar compounds present in the leaves and stems of A. paniculata as the main active ingredients. This finding forms a strong basis for further studies on screening of A. paniculata extracts for the exploration of potent antibacterial agents. 2009-05-12 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/4479/1/3.pdf Sule, Abubakar and Abd. Samah, Othman and Omar, Muhammad Nor and Ahmed, Qamar Uddin (2009) In vitro screening of Andrographis paniculata (Hempedu bumi) for antibacterial activity using disc diffusion method. In: 23rd Malaysian Society of Pharmacology and Physiology Scientific (MSPP) Meeting (Theme: New Technologies for improving quality of life), 12th-13th May 2009, Grand Seasons Hotel, Kuala Lumpur, Malaysia. . http://pkukmweb.ukm.my/~mspp2009.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Sule, Abubakar Abd. Samah, Othman Omar, Muhammad Nor Ahmed, Qamar Uddin In vitro screening of Andrographis paniculata (Hempedu bumi) for antibacterial activity using disc diffusion method
description	The increasing prevalence in multidrug resistant strains of bacteria and the recent appearance of strains with reduced susceptibility to antibiotics raises the specter of untreatable bacterial infections and adds urgency to the search for new infection-fighting strategies1. The plant Andrographis paniculata (Hempedu Bumi) is used in traditional medicinal practice for the treatment of various chronic and infectious diseases some of bacterial origin2.. This study was carried out to evaluate the antibacterial activity of organic extracts of A. paniculata leaves and stems against 8 pathogenic bacterial strains: Staphylococcus aureus, Streptococcus pyogenes, Bacillus anthrax Micrococcus luteus, Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas aeroginosa and Proteus mirabilis using disc diffusion method. Dried ground powdered leaves and stems were extracted with petroleum ether, dichloromethane, ethyl acetate, acetone, ethanol and finally with methanol using the soxhlet extraction. The result obtained vividly revealed that the different extracts differed significantly in their antibacterial properties with Dichloromethane extract being the most effective followed by acetone, ethyl acetate, methanol, petroleum ether and lastly ethanol extract. In general, the leaves exhibited strong antibacterial activity than the stems extracts; but all the extracts exerted very good broad spectrum activity against all tested microorganisms. The results explicitly suggest that there might be polar compounds present in the leaves and stems of A. paniculata as the main active ingredients. This finding forms a strong basis for further studies on screening of A. paniculata extracts for the exploration of potent antibacterial agents.
format	Conference or Workshop Item
author	Sule, Abubakar Abd. Samah, Othman Omar, Muhammad Nor Ahmed, Qamar Uddin
author_facet	Sule, Abubakar Abd. Samah, Othman Omar, Muhammad Nor Ahmed, Qamar Uddin
author_sort	Sule, Abubakar
title	In vitro screening of Andrographis paniculata (Hempedu bumi) for antibacterial activity using disc diffusion method
title_short	In vitro screening of Andrographis paniculata (Hempedu bumi) for antibacterial activity using disc diffusion method
title_full	In vitro screening of Andrographis paniculata (Hempedu bumi) for antibacterial activity using disc diffusion method
title_fullStr	In vitro screening of Andrographis paniculata (Hempedu bumi) for antibacterial activity using disc diffusion method
title_full_unstemmed	In vitro screening of Andrographis paniculata (Hempedu bumi) for antibacterial activity using disc diffusion method
title_sort	in vitro screening of andrographis paniculata (hempedu bumi) for antibacterial activity using disc diffusion method
publishDate	2009
url	http://irep.iium.edu.my/4479/ http://irep.iium.edu.my/4479/ http://irep.iium.edu.my/4479/1/3.pdf
first_indexed	2023-09-18T20:12:43Z
last_indexed	2023-09-18T20:12:43Z
_version_	1777407584480788480

In vitro screening of Andrographis paniculata (Hempedu bumi) for antibacterial activity using disc diffusion method

Similar Items