Cementoblastic lineage formation in the cross-talk between stem cells of human exfoliated deciduous teeth and epithelial rests of Malassez cells

Objectives The purpose of this study was to evaluate the synergistic effect of epithelial rests of Malassez cells (ERM) and transforming growth factor-β1 (TGF-β1) on proliferation, cementogenic and osteogenic differentiation of stem cells derived from human exfoliated deciduous teeth (SHED). Mater...

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Main Authors:	Farea, Manal, Husein, Adam, Halim, Ahmad Sukari, Berahim, Zurairah, Abdullah Nurul, Asma, Mokhtar@Makhtar, Khairani Idah, Mokhtar, Kasmawati
Format:	Article
Language:	English
Published:	Springer Berlin Heidelberg 2015
Subjects:	RK Dentistry
Online Access:	http://irep.iium.edu.my/44845/ http://irep.iium.edu.my/44845/ http://irep.iium.edu.my/44845/ http://irep.iium.edu.my/44845/1/2015-Clin_Oral_Invest-Manal_et_al_Cementoblastic_lineage_formation_in_the_cross_talk_between___stem_cells_of_human_exfoliated_deciduous_teeth_and_epithelial_rests_of___Mal.pdf

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spelling	iium-448452016-02-26T01:06:56Z http://irep.iium.edu.my/44845/ Cementoblastic lineage formation in the cross-talk between stem cells of human exfoliated deciduous teeth and epithelial rests of Malassez cells Farea, Manal Husein, Adam Halim, Ahmad Sukari Berahim, Zurairah Abdullah Nurul, Asma Mokhtar@Makhtar, Khairani Idah Mokhtar, Kasmawati RK Dentistry Objectives The purpose of this study was to evaluate the synergistic effect of epithelial rests of Malassez cells (ERM) and transforming growth factor-β1 (TGF-β1) on proliferation, cementogenic and osteogenic differentiation of stem cells derived from human exfoliated deciduous teeth (SHED). Materials and methods SHED were co-cultured with ERM with/without TGF-β1. Then, SHED proliferation, morphological appearance, alkaline phosphatase (ALP) activity, mineralization behaviour and gene/protein expression of cemento/osteoblastic phenotype were evaluated. Results TGF-β1 enhanced SHED proliferation when either cultured alone or co-cultured with ERM. ERM induced the cementoblastic differentiation of SHED which was significantly accelerated when treated with TGF-β1. This activity was demonstrated by high ALP activity, strong mineral deposition and upregulation of cementum/bone-related gene and protein expressions (i.e. ALP, collagen type I, bone sialoprotein, osteocalcin and cementum attachment protein). Conclusions ERM were able to induce SHED differentiation along the cemento/osteoblastic lineage that was triggered in the presence of TGF-β1. Clinical relevance The cemento/osteoblastic differentiation capability of SHED possesses a therapeutic potential in endodontic and periodontal tissue engineering. Springer Berlin Heidelberg 2015-09-22 Article PeerReviewed application/pdf en http://irep.iium.edu.my/44845/1/2015-Clin_Oral_Invest-Manal_et_al_Cementoblastic_lineage_formation_in_the_cross_talk_between___stem_cells_of_human_exfoliated_deciduous_teeth_and_epithelial_rests_of___Mal.pdf Farea, Manal and Husein, Adam and Halim, Ahmad Sukari and Berahim, Zurairah and Abdullah Nurul, Asma and Mokhtar@Makhtar, Khairani Idah and Mokhtar, Kasmawati (2015) Cementoblastic lineage formation in the cross-talk between stem cells of human exfoliated deciduous teeth and epithelial rests of Malassez cells. Clinical Oral Investigation. ISSN 1436-3771 (O), 1432-6981 (P) (In Press) http://link.springer.com/article/10.1007%2Fs00784-015-1601-6 10.1007/s00784-015-1601-6
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RK Dentistry
spellingShingle	RK Dentistry Farea, Manal Husein, Adam Halim, Ahmad Sukari Berahim, Zurairah Abdullah Nurul, Asma Mokhtar@Makhtar, Khairani Idah Mokhtar, Kasmawati Cementoblastic lineage formation in the cross-talk between stem cells of human exfoliated deciduous teeth and epithelial rests of Malassez cells
description	Objectives The purpose of this study was to evaluate the synergistic effect of epithelial rests of Malassez cells (ERM) and transforming growth factor-β1 (TGF-β1) on proliferation, cementogenic and osteogenic differentiation of stem cells derived from human exfoliated deciduous teeth (SHED). Materials and methods SHED were co-cultured with ERM with/without TGF-β1. Then, SHED proliferation, morphological appearance, alkaline phosphatase (ALP) activity, mineralization behaviour and gene/protein expression of cemento/osteoblastic phenotype were evaluated. Results TGF-β1 enhanced SHED proliferation when either cultured alone or co-cultured with ERM. ERM induced the cementoblastic differentiation of SHED which was significantly accelerated when treated with TGF-β1. This activity was demonstrated by high ALP activity, strong mineral deposition and upregulation of cementum/bone-related gene and protein expressions (i.e. ALP, collagen type I, bone sialoprotein, osteocalcin and cementum attachment protein). Conclusions ERM were able to induce SHED differentiation along the cemento/osteoblastic lineage that was triggered in the presence of TGF-β1. Clinical relevance The cemento/osteoblastic differentiation capability of SHED possesses a therapeutic potential in endodontic and periodontal tissue engineering.
format	Article
author	Farea, Manal Husein, Adam Halim, Ahmad Sukari Berahim, Zurairah Abdullah Nurul, Asma Mokhtar@Makhtar, Khairani Idah Mokhtar, Kasmawati
author_facet	Farea, Manal Husein, Adam Halim, Ahmad Sukari Berahim, Zurairah Abdullah Nurul, Asma Mokhtar@Makhtar, Khairani Idah Mokhtar, Kasmawati
author_sort	Farea, Manal
title	Cementoblastic lineage formation in the cross-talk between stem cells of human exfoliated deciduous teeth and epithelial rests of Malassez cells
title_short	Cementoblastic lineage formation in the cross-talk between stem cells of human exfoliated deciduous teeth and epithelial rests of Malassez cells
title_full	Cementoblastic lineage formation in the cross-talk between stem cells of human exfoliated deciduous teeth and epithelial rests of Malassez cells
title_fullStr	Cementoblastic lineage formation in the cross-talk between stem cells of human exfoliated deciduous teeth and epithelial rests of Malassez cells
title_full_unstemmed	Cementoblastic lineage formation in the cross-talk between stem cells of human exfoliated deciduous teeth and epithelial rests of Malassez cells
title_sort	cementoblastic lineage formation in the cross-talk between stem cells of human exfoliated deciduous teeth and epithelial rests of malassez cells
publisher	Springer Berlin Heidelberg
publishDate	2015
url	http://irep.iium.edu.my/44845/ http://irep.iium.edu.my/44845/ http://irep.iium.edu.my/44845/ http://irep.iium.edu.my/44845/1/2015-Clin_Oral_Invest-Manal_et_al_Cementoblastic_lineage_formation_in_the_cross_talk_between___stem_cells_of_human_exfoliated_deciduous_teeth_and_epithelial_rests_of___Mal.pdf
first_indexed	2023-09-18T21:03:46Z
last_indexed	2023-09-18T21:03:46Z
_version_	1777410796488228864

Cementoblastic lineage formation in the cross-talk between stem cells of human exfoliated deciduous teeth and epithelial rests of Malassez cells

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