A novel animal model for subcutaneous soft tissue infection using temporally neutropenic lys-EGFP mice
Neutrophils play an important role against bacterial infection, mainly methicillin resistant Staphylococcus aureus (MRSA). Therefore, we developed an animal model to simultaneously monitor bacterial colonization and neutrophil migration in vivo. Using lys-EGFP C57BL/6 mice, we initially rendered the...
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| Format: | Conference or Workshop Item |
| Language: | English English |
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2015
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| Online Access: | http://irep.iium.edu.my/44920/ http://irep.iium.edu.my/44920/ http://irep.iium.edu.my/44920/ http://irep.iium.edu.my/44920/1/A_Novel_Animal_Model_for_Subcutaneous_Soft.pdf http://irep.iium.edu.my/44920/4/ascc_proceeeding_cove%2C_copyright%2C_content.pdf |
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iium-44920 |
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eprints |
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iium-449202016-07-19T03:13:58Z http://irep.iium.edu.my/44920/ A novel animal model for subcutaneous soft tissue infection using temporally neutropenic lys-EGFP mice Zulaziz, N. Azhim, Azran Miyazaki, H. Kinoshita, M. Himeno, N. Saitoh, D. Morimoto, Y. TP248.13 Biotechnology Neutrophils play an important role against bacterial infection, mainly methicillin resistant Staphylococcus aureus (MRSA). Therefore, we developed an animal model to simultaneously monitor bacterial colonization and neutrophil migration in vivo. Using lys-EGFP C57BL/6 mice, we initially rendered the mice temporally neutropenic using cyclophosphamide (CPM) treatment (300mg/kg or 375mg/kg). Later, bioluminescent MRSA (Xen31, PerkinElmer) were subcutaneously injected (1.0×107 CFU) into the dorsal skin of the neutropenic lys-EGFP mice. The mice were then administered either saline (control group), or vancomycin (66mg/kg, treated group) consecutively for three days. For the evaluation of MRSA activity and neutrophil accumulation, an in vivo imaging system (LAS-4000, GE) was performed. Our results demonstrated that vancomycin is capable of killing bacterial cells and it also promotes inflammation. In addition, the rate of neutrophil regeneration after being suppressed by CPM is dependent on the dose of CPM. We could conclude that vancomycin is capable of inducing inflammation but the effects of the drug could only be observed if the immune system is adequately suppressed. 2015 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/44920/1/A_Novel_Animal_Model_for_Subcutaneous_Soft.pdf application/pdf en http://irep.iium.edu.my/44920/4/ascc_proceeeding_cove%2C_copyright%2C_content.pdf Zulaziz, N. and Azhim, Azran and Miyazaki, H. and Kinoshita, M. and Himeno, N. and Saitoh, D. and Morimoto, Y. (2015) A novel animal model for subcutaneous soft tissue infection using temporally neutropenic lys-EGFP mice. In: The 10th Asian Control Conference (ASCC 2015), 31 May-3 June 2015, Kota Kinabalu, Sabah. http://dx.doi.org/10.1109/ASCC.2015.7244500 doi:10.1109/ASCC.2015.7244500 |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English English |
| topic |
TP248.13 Biotechnology |
| spellingShingle |
TP248.13 Biotechnology Zulaziz, N. Azhim, Azran Miyazaki, H. Kinoshita, M. Himeno, N. Saitoh, D. Morimoto, Y. A novel animal model for subcutaneous soft tissue infection using temporally neutropenic lys-EGFP mice |
| description |
Neutrophils play an important role against bacterial infection, mainly methicillin resistant Staphylococcus aureus (MRSA). Therefore, we developed an animal model to simultaneously monitor bacterial colonization and neutrophil migration in vivo. Using lys-EGFP C57BL/6 mice, we initially rendered the mice temporally neutropenic using cyclophosphamide (CPM) treatment (300mg/kg or 375mg/kg). Later, bioluminescent MRSA (Xen31, PerkinElmer) were subcutaneously injected (1.0×107 CFU) into the dorsal skin of the neutropenic lys-EGFP mice. The mice were then administered either saline (control group), or vancomycin (66mg/kg, treated group) consecutively for three days. For the evaluation of MRSA activity and neutrophil accumulation, an in vivo imaging system (LAS-4000, GE) was performed. Our results demonstrated that vancomycin is capable of killing bacterial cells and it also promotes inflammation. In addition, the rate of neutrophil regeneration after being suppressed by CPM is dependent on the dose of CPM. We could conclude that vancomycin is capable of inducing inflammation but the effects of the drug could only be observed if the immune system is adequately suppressed. |
| format |
Conference or Workshop Item |
| author |
Zulaziz, N. Azhim, Azran Miyazaki, H. Kinoshita, M. Himeno, N. Saitoh, D. Morimoto, Y. |
| author_facet |
Zulaziz, N. Azhim, Azran Miyazaki, H. Kinoshita, M. Himeno, N. Saitoh, D. Morimoto, Y. |
| author_sort |
Zulaziz, N. |
| title |
A novel animal model for subcutaneous soft tissue infection using temporally neutropenic lys-EGFP mice |
| title_short |
A novel animal model for subcutaneous soft tissue infection using temporally neutropenic lys-EGFP mice |
| title_full |
A novel animal model for subcutaneous soft tissue infection using temporally neutropenic lys-EGFP mice |
| title_fullStr |
A novel animal model for subcutaneous soft tissue infection using temporally neutropenic lys-EGFP mice |
| title_full_unstemmed |
A novel animal model for subcutaneous soft tissue infection using temporally neutropenic lys-EGFP mice |
| title_sort |
novel animal model for subcutaneous soft tissue infection using temporally neutropenic lys-egfp mice |
| publishDate |
2015 |
| url |
http://irep.iium.edu.my/44920/ http://irep.iium.edu.my/44920/ http://irep.iium.edu.my/44920/ http://irep.iium.edu.my/44920/1/A_Novel_Animal_Model_for_Subcutaneous_Soft.pdf http://irep.iium.edu.my/44920/4/ascc_proceeeding_cove%2C_copyright%2C_content.pdf |
| first_indexed |
2023-09-18T21:03:52Z |
| last_indexed |
2023-09-18T21:03:52Z |
| _version_ |
1777410803419316224 |