Decellularized bovine meniscus in morphological assessment prior to bioscaffold preparation

Decellularization is a process of tissue treatment targeting cell removal. Sonication system was developed in order to decellularize meniscus tissues. The samples were sonicated in 0.1% sodium dodecyl sulphate (SDS) for 10 hours and at 40 kHz ultrasound frequency. All the samples were structurally e...

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Main Authors:	Norzarini, A., Azhim, Azran, Ushida, T.
Format:	Conference or Workshop Item
Language:	English English
Published:	2015
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/44922/ http://irep.iium.edu.my/44922/ http://irep.iium.edu.my/44922/ http://irep.iium.edu.my/44922/1/Decellularized_Bovine_Meniscus_in_Morphological.pdf http://irep.iium.edu.my/44922/4/ascc_proceeeding_cove%2C_copyright%2C_content.pdf

id	iium-44922
recordtype	eprints
spelling	iium-449222016-06-27T16:04:40Z http://irep.iium.edu.my/44922/ Decellularized bovine meniscus in morphological assessment prior to bioscaffold preparation Norzarini, A. Azhim, Azran Ushida, T. TP248.13 Biotechnology Decellularization is a process of tissue treatment targeting cell removal. Sonication system was developed in order to decellularize meniscus tissues. The samples were sonicated in 0.1% sodium dodecyl sulphate (SDS) for 10 hours and at 40 kHz ultrasound frequency. All the samples were structurally examined using van Gieson, Picrosirius red, Safranin-O/Fast green staining, and scanning electron microscopic (SEM) observation. Histological analysis of sonication treated-samples by van Gieson staining demonstrated complete nuclei removal compared to the control samples. The Picrosirius red and Safranin-O/Fast green staining indicate the preservation of collagen and glycosaminoglycans (GAGs) structure, respectively. In addition, the morphological observation by SEM shows the availability of micropores on the surface of decellularized sample. Consequently, the sonication decellularization treatment did not affect extracellular matrix (ECM) properties, while forming micropores on the surface of meniscus tissues. This made it possible to proceed in other fulfillment of bioscaffold preparation. 2015 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/44922/1/Decellularized_Bovine_Meniscus_in_Morphological.pdf application/pdf en http://irep.iium.edu.my/44922/4/ascc_proceeeding_cove%2C_copyright%2C_content.pdf Norzarini, A. and Azhim, Azran and Ushida, T. (2015) Decellularized bovine meniscus in morphological assessment prior to bioscaffold preparation. In: The 10th Asian Control Conference (ASCC 2015), 31 May - 3 June 2015, Kota Kinabalu, Sabah. http://dx.doi.org/10.1109/ASCC.2015.7244681 doi:10.1109/ASCC.2015.7244681
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Norzarini, A. Azhim, Azran Ushida, T. Decellularized bovine meniscus in morphological assessment prior to bioscaffold preparation
description	Decellularization is a process of tissue treatment targeting cell removal. Sonication system was developed in order to decellularize meniscus tissues. The samples were sonicated in 0.1% sodium dodecyl sulphate (SDS) for 10 hours and at 40 kHz ultrasound frequency. All the samples were structurally examined using van Gieson, Picrosirius red, Safranin-O/Fast green staining, and scanning electron microscopic (SEM) observation. Histological analysis of sonication treated-samples by van Gieson staining demonstrated complete nuclei removal compared to the control samples. The Picrosirius red and Safranin-O/Fast green staining indicate the preservation of collagen and glycosaminoglycans (GAGs) structure, respectively. In addition, the morphological observation by SEM shows the availability of micropores on the surface of decellularized sample. Consequently, the sonication decellularization treatment did not affect extracellular matrix (ECM) properties, while forming micropores on the surface of meniscus tissues. This made it possible to proceed in other fulfillment of bioscaffold preparation.
format	Conference or Workshop Item
author	Norzarini, A. Azhim, Azran Ushida, T.
author_facet	Norzarini, A. Azhim, Azran Ushida, T.
author_sort	Norzarini, A.
title	Decellularized bovine meniscus in morphological assessment prior to bioscaffold preparation
title_short	Decellularized bovine meniscus in morphological assessment prior to bioscaffold preparation
title_full	Decellularized bovine meniscus in morphological assessment prior to bioscaffold preparation
title_fullStr	Decellularized bovine meniscus in morphological assessment prior to bioscaffold preparation
title_full_unstemmed	Decellularized bovine meniscus in morphological assessment prior to bioscaffold preparation
title_sort	decellularized bovine meniscus in morphological assessment prior to bioscaffold preparation
publishDate	2015
url	http://irep.iium.edu.my/44922/ http://irep.iium.edu.my/44922/ http://irep.iium.edu.my/44922/ http://irep.iium.edu.my/44922/1/Decellularized_Bovine_Meniscus_in_Morphological.pdf http://irep.iium.edu.my/44922/4/ascc_proceeeding_cove%2C_copyright%2C_content.pdf
first_indexed	2023-09-18T21:03:53Z
last_indexed	2023-09-18T21:03:53Z
_version_	1777410803695091712

Decellularized bovine meniscus in morphological assessment prior to bioscaffold preparation

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