In vitro recellularization of aorta scaffolds prepared by sonication treatment

Sonication treatment is used in the preparation of bioscaffolds that was able to support repopulation of vascular smooth muscle cells (VSMCs) upon cell-seeding. The aim of this study is to investigate the ability of sonicatedly decellularized tissue to repopulate VSMCs after 6 days of cell-seeding....

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Main Authors:	Syazwani, N., Ushida, T., Azhim, Azran
Format:	Conference or Workshop Item
Language:	English English
Published:	2015
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/44926/ http://irep.iium.edu.my/44926/ http://irep.iium.edu.my/44926/ http://irep.iium.edu.my/44926/1/In_vitro_recellularization_of_aorta_scaffolds_prepared_by_sonication_treatment.pdf http://irep.iium.edu.my/44926/4/ascc_proceeeding_cove%2C_copyright%2C_content.pdf

id	iium-44926
recordtype	eprints
spelling	iium-449262016-05-23T05:36:31Z http://irep.iium.edu.my/44926/ In vitro recellularization of aorta scaffolds prepared by sonication treatment Syazwani, N. Ushida, T. Azhim, Azran TP248.13 Biotechnology Sonication treatment is used in the preparation of bioscaffolds that was able to support repopulation of vascular smooth muscle cells (VSMCs) upon cell-seeding. The aim of this study is to investigate the ability of sonicatedly decellularized tissue to repopulate VSMCs after 6 days of cell-seeding. In this study, sample of aorta tissues are decellularized by sonication treatment in 0.1% and 2% sodium dodecyl sulfate (SDS) detergent for 10 hours. It was followed by washing process with PBS solution for 5 days. Decellularized aorta tissues are then cell seeded with VSMCs by static seeding in 96-well plate containing Dulbecco's Modified Eagle Medium (DMEM) at 37°C. The infiltrations of VSMCs onto decellularized tissues are evaluated by comparison of Hematoxylin-Eosin (H-E) staining at 0 and 6 days of cell-seeding. The histological results of cell-seeding showed that VSMCs are able to infiltrate onto the decellularized tissues. From the results, sonicatedly decellularized tissue treated in 0.1% and 2% SDS, seeded with VSMCs showed infiltration depth of 0.43 mm and 0.35 mm, respectively. Hence, a sonicated decellularized tissue treated with 0.1% and 2% SDS was shown to support the repopulation of VSMCs. 2015 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/44926/1/In_vitro_recellularization_of_aorta_scaffolds_prepared_by_sonication_treatment.pdf application/pdf en http://irep.iium.edu.my/44926/4/ascc_proceeeding_cove%2C_copyright%2C_content.pdf Syazwani, N. and Ushida, T. and Azhim, Azran (2015) In vitro recellularization of aorta scaffolds prepared by sonication treatment. In: The 10th Asian Control Conference (ASCC 2015), 31 May - 3 June 2015, Kota Kinabalu, Sabah. http://dx.doi.org/10.1109/ASCC.2015.7244769 doi:10.1109/ASCC.2015.7244769
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Syazwani, N. Ushida, T. Azhim, Azran In vitro recellularization of aorta scaffolds prepared by sonication treatment
description	Sonication treatment is used in the preparation of bioscaffolds that was able to support repopulation of vascular smooth muscle cells (VSMCs) upon cell-seeding. The aim of this study is to investigate the ability of sonicatedly decellularized tissue to repopulate VSMCs after 6 days of cell-seeding. In this study, sample of aorta tissues are decellularized by sonication treatment in 0.1% and 2% sodium dodecyl sulfate (SDS) detergent for 10 hours. It was followed by washing process with PBS solution for 5 days. Decellularized aorta tissues are then cell seeded with VSMCs by static seeding in 96-well plate containing Dulbecco's Modified Eagle Medium (DMEM) at 37°C. The infiltrations of VSMCs onto decellularized tissues are evaluated by comparison of Hematoxylin-Eosin (H-E) staining at 0 and 6 days of cell-seeding. The histological results of cell-seeding showed that VSMCs are able to infiltrate onto the decellularized tissues. From the results, sonicatedly decellularized tissue treated in 0.1% and 2% SDS, seeded with VSMCs showed infiltration depth of 0.43 mm and 0.35 mm, respectively. Hence, a sonicated decellularized tissue treated with 0.1% and 2% SDS was shown to support the repopulation of VSMCs.
format	Conference or Workshop Item
author	Syazwani, N. Ushida, T. Azhim, Azran
author_facet	Syazwani, N. Ushida, T. Azhim, Azran
author_sort	Syazwani, N.
title	In vitro recellularization of aorta scaffolds prepared by sonication treatment
title_short	In vitro recellularization of aorta scaffolds prepared by sonication treatment
title_full	In vitro recellularization of aorta scaffolds prepared by sonication treatment
title_fullStr	In vitro recellularization of aorta scaffolds prepared by sonication treatment
title_full_unstemmed	In vitro recellularization of aorta scaffolds prepared by sonication treatment
title_sort	in vitro recellularization of aorta scaffolds prepared by sonication treatment
publishDate	2015
url	http://irep.iium.edu.my/44926/ http://irep.iium.edu.my/44926/ http://irep.iium.edu.my/44926/ http://irep.iium.edu.my/44926/1/In_vitro_recellularization_of_aorta_scaffolds_prepared_by_sonication_treatment.pdf http://irep.iium.edu.my/44926/4/ascc_proceeeding_cove%2C_copyright%2C_content.pdf
first_indexed	2023-09-18T21:03:53Z
last_indexed	2023-09-18T21:03:53Z
_version_	1777410804148076544

In vitro recellularization of aorta scaffolds prepared by sonication treatment

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