Preparation and characterization of resiquimod-loaded microsponges

INTRODUCTION Microsponges are porous microspheres, which can be used as a polymeric delivery system to deliver active pharmaceutical ingredient such as resiquimod in a controlled release manner. The objectives of this study were to encapsulate and characterize resiquimod in the microsponges. M...

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Main Authors:	Ab. Hadi, Hazrina, Awadh, Ammar Ihsan JawaD
Format:	Conference or Workshop Item
Language:	English
Published:	2015
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/45044/ http://irep.iium.edu.my/45044/1/45044.pdf

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recordtype	eprints
spelling	iium-450442018-01-03T02:25:01Z http://irep.iium.edu.my/45044/ Preparation and characterization of resiquimod-loaded microsponges Ab. Hadi, Hazrina Awadh, Ammar Ihsan JawaD RS Pharmacy and materia medica INTRODUCTION Microsponges are porous microspheres, which can be used as a polymeric delivery system to deliver active pharmaceutical ingredient such as resiquimod in a controlled release manner. The objectives of this study were to encapsulate and characterize resiquimod in the microsponges. MATERIALS AND METHODS 100mg of ethylcellulose and 1mg of resiquimod was dissolved in dichlromethane (DCM) or ethylacetate (EA) and was poured into 1% of polyvinyl alcohol (PVA). The solvent was evaporated in a fume hood. Particle size of microsponges were determined by BT-9300H Laser Particle Size Analyzer. The microspheres were coated with gold palladium alloy and observed with a field emission scanning electron microscope FESEM at an intensity of 10kV. RESULTS AND DISCUSSION With 10 mL DCM with 20 mL PVA 1%, the entrapment efficiency (EE) of resiquimod is 31.32 ± 2.22. The amount of DCM used in the microsponge preparation decreased to 5 mL with 20 mL PVA 1% in which the %EE was found to be high (32.58 ± 1.76%). The preparation with 5 mL EA and 5 mL DCM with 20 mL PVA 1% resulted in lower % EE (23.75 ± 0.30). This might be due to the hardening time of microspheres is faster with EA compared to DCM. The microsponges prepared have homogeneous particle size ranging from 3.82 to 4.21 µm. The surface of microspheres prepared by EA are mainly appeared to be wrinkle, meanwhile microspheres prepared by DCM appeared to have smooth surfaces which indicates rapid polymer precipitation. FigFig. 1. FESEM results of microsponges prepared using DCM in dispersion phase as A) 1 mL, B) 2.5 mL C) 5 mL D) 10 mL. CONCLUSIONS The type and amount of organic solvents play a vital role in the resulted microspheres. The highest % EE and optimum particle size was obtained with low amount of organic solvents. 2015 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/45044/1/45044.pdf Ab. Hadi, Hazrina and Awadh, Ammar Ihsan JawaD (2015) Preparation and characterization of resiquimod-loaded microsponges. In: The 6th APS International PharmSci 2015, 7th-9th Sept. 2015, East Midlands Conference Centre, Nottingham. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Ab. Hadi, Hazrina Awadh, Ammar Ihsan JawaD Preparation and characterization of resiquimod-loaded microsponges
description	INTRODUCTION Microsponges are porous microspheres, which can be used as a polymeric delivery system to deliver active pharmaceutical ingredient such as resiquimod in a controlled release manner. The objectives of this study were to encapsulate and characterize resiquimod in the microsponges. MATERIALS AND METHODS 100mg of ethylcellulose and 1mg of resiquimod was dissolved in dichlromethane (DCM) or ethylacetate (EA) and was poured into 1% of polyvinyl alcohol (PVA). The solvent was evaporated in a fume hood. Particle size of microsponges were determined by BT-9300H Laser Particle Size Analyzer. The microspheres were coated with gold palladium alloy and observed with a field emission scanning electron microscope FESEM at an intensity of 10kV. RESULTS AND DISCUSSION With 10 mL DCM with 20 mL PVA 1%, the entrapment efficiency (EE) of resiquimod is 31.32 ± 2.22. The amount of DCM used in the microsponge preparation decreased to 5 mL with 20 mL PVA 1% in which the %EE was found to be high (32.58 ± 1.76%). The preparation with 5 mL EA and 5 mL DCM with 20 mL PVA 1% resulted in lower % EE (23.75 ± 0.30). This might be due to the hardening time of microspheres is faster with EA compared to DCM. The microsponges prepared have homogeneous particle size ranging from 3.82 to 4.21 µm. The surface of microspheres prepared by EA are mainly appeared to be wrinkle, meanwhile microspheres prepared by DCM appeared to have smooth surfaces which indicates rapid polymer precipitation. FigFig. 1. FESEM results of microsponges prepared using DCM in dispersion phase as A) 1 mL, B) 2.5 mL C) 5 mL D) 10 mL. CONCLUSIONS The type and amount of organic solvents play a vital role in the resulted microspheres. The highest % EE and optimum particle size was obtained with low amount of organic solvents.
format	Conference or Workshop Item
author	Ab. Hadi, Hazrina Awadh, Ammar Ihsan JawaD
author_facet	Ab. Hadi, Hazrina Awadh, Ammar Ihsan JawaD
author_sort	Ab. Hadi, Hazrina
title	Preparation and characterization of resiquimod-loaded microsponges
title_short	Preparation and characterization of resiquimod-loaded microsponges
title_full	Preparation and characterization of resiquimod-loaded microsponges
title_fullStr	Preparation and characterization of resiquimod-loaded microsponges
title_full_unstemmed	Preparation and characterization of resiquimod-loaded microsponges
title_sort	preparation and characterization of resiquimod-loaded microsponges
publishDate	2015
url	http://irep.iium.edu.my/45044/ http://irep.iium.edu.my/45044/1/45044.pdf
first_indexed	2023-09-18T21:04:04Z
last_indexed	2023-09-18T21:04:04Z
_version_	1777410815383568384

Preparation and characterization of resiquimod-loaded microsponges

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