Phytochemical and biological features of Phyllanthus niruri and Phyllanthus urinaria harvested at different growth stages revealed by H-1 NMR-based metabolomics

tSeveral studies have suggested that plants are potential sources of bioactive compounds. Phyllanthus isa plant genus that has been used in traditional medicine due to its phytomedicinal metabolites content.The variation between two Phyllanthus species (P. niruri and P. urinaria) was studied using p...

Full description

Bibliographic Details
Main Authors:	Mediani, Ahmed, Abas, Faridah, Khatib, Alfi, Tan, CP, Ismail, IS, Shaari, Khozirah, Ismail, Amin, Lajis, NH
Format:	Article
Language:	English
Published:	Elsevier 2015
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/45216/ http://irep.iium.edu.my/45216/ http://irep.iium.edu.my/45216/1/mediani_et_al._2015.pdf

id	iium-45216
recordtype	eprints
spelling	iium-452162017-08-16T03:26:27Z http://irep.iium.edu.my/45216/ Phytochemical and biological features of Phyllanthus niruri and Phyllanthus urinaria harvested at different growth stages revealed by H-1 NMR-based metabolomics Mediani, Ahmed Abas, Faridah Khatib, Alfi Tan, CP Ismail, IS Shaari, Khozirah Ismail, Amin Lajis, NH QD Chemistry tSeveral studies have suggested that plants are potential sources of bioactive compounds. Phyllanthus isa plant genus that has been used in traditional medicine due to its phytomedicinal metabolites content.The variation between two Phyllanthus species (P. niruri and P. urinaria) was studied using proton nuclearmagnetic resonance (1H NMR) combined with multivariate data analysis (MVDA). The total phenoliccontent (TPC), DPPH radical scavenging activity and �-glucosidase inhibitory activity of the Phyllanthusspecies were also evaluated and correlated with their phytochemical constituents at different growthstages (8, 10 and 12 weeks) using partial least square regression (PLS). Principal component analysis(PCA) and PLS indicated separation between the two species based on the identified metabolites and thescreened bioactivities. A comparison of the two species indicated that P. urinaria was separated from P.niruri due to its larger quantity of fatty and amino acids, choline, phyllanthin and sucrose. However, P.niruri contained higher quantities of hypophyllanthin and phenolic compounds. The loading column plot,which was used to compare the P. niruri at different growth stages, indicated that the eight-week-oldplant contained a higher amount of fatty acids, amino acids (leucine and alanine), phyllanthin and choline.The dominant substances in the P. niruri at 10 weeks of growth by PC1 were identified as hypophyllanthin,malic acid, sucrose, and identified phenolics. The 12 week sample was differentiated by its higher sugarscontents as well as malic acid and leucine. The harvested samples of both Phyllanthus species at ten weeksof age exhibited significant bioactivities with the highest content and number of metabolites. Elsevier 2015-09-13 Article PeerReviewed application/pdf en http://irep.iium.edu.my/45216/1/mediani_et_al._2015.pdf Mediani, Ahmed and Abas, Faridah and Khatib, Alfi and Tan, CP and Ismail, IS and Shaari, Khozirah and Ismail, Amin and Lajis, NH (2015) Phytochemical and biological features of Phyllanthus niruri and Phyllanthus urinaria harvested at different growth stages revealed by H-1 NMR-based metabolomics. Industrial Crops and Products, 77. pp. 602-613. ISSN 0926-6690 http://www.journals.elsevier.com/industrial-crops-and-products/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Mediani, Ahmed Abas, Faridah Khatib, Alfi Tan, CP Ismail, IS Shaari, Khozirah Ismail, Amin Lajis, NH Phytochemical and biological features of Phyllanthus niruri and Phyllanthus urinaria harvested at different growth stages revealed by H-1 NMR-based metabolomics
description	tSeveral studies have suggested that plants are potential sources of bioactive compounds. Phyllanthus isa plant genus that has been used in traditional medicine due to its phytomedicinal metabolites content.The variation between two Phyllanthus species (P. niruri and P. urinaria) was studied using proton nuclearmagnetic resonance (1H NMR) combined with multivariate data analysis (MVDA). The total phenoliccontent (TPC), DPPH radical scavenging activity and �-glucosidase inhibitory activity of the Phyllanthusspecies were also evaluated and correlated with their phytochemical constituents at different growthstages (8, 10 and 12 weeks) using partial least square regression (PLS). Principal component analysis(PCA) and PLS indicated separation between the two species based on the identified metabolites and thescreened bioactivities. A comparison of the two species indicated that P. urinaria was separated from P.niruri due to its larger quantity of fatty and amino acids, choline, phyllanthin and sucrose. However, P.niruri contained higher quantities of hypophyllanthin and phenolic compounds. The loading column plot,which was used to compare the P. niruri at different growth stages, indicated that the eight-week-oldplant contained a higher amount of fatty acids, amino acids (leucine and alanine), phyllanthin and choline.The dominant substances in the P. niruri at 10 weeks of growth by PC1 were identified as hypophyllanthin,malic acid, sucrose, and identified phenolics. The 12 week sample was differentiated by its higher sugarscontents as well as malic acid and leucine. The harvested samples of both Phyllanthus species at ten weeksof age exhibited significant bioactivities with the highest content and number of metabolites.
format	Article
author	Mediani, Ahmed Abas, Faridah Khatib, Alfi Tan, CP Ismail, IS Shaari, Khozirah Ismail, Amin Lajis, NH
author_facet	Mediani, Ahmed Abas, Faridah Khatib, Alfi Tan, CP Ismail, IS Shaari, Khozirah Ismail, Amin Lajis, NH
author_sort	Mediani, Ahmed
title	Phytochemical and biological features of Phyllanthus niruri and Phyllanthus urinaria harvested at different growth stages revealed by H-1 NMR-based metabolomics
title_short	Phytochemical and biological features of Phyllanthus niruri and Phyllanthus urinaria harvested at different growth stages revealed by H-1 NMR-based metabolomics
title_full	Phytochemical and biological features of Phyllanthus niruri and Phyllanthus urinaria harvested at different growth stages revealed by H-1 NMR-based metabolomics
title_fullStr	Phytochemical and biological features of Phyllanthus niruri and Phyllanthus urinaria harvested at different growth stages revealed by H-1 NMR-based metabolomics
title_full_unstemmed	Phytochemical and biological features of Phyllanthus niruri and Phyllanthus urinaria harvested at different growth stages revealed by H-1 NMR-based metabolomics
title_sort	phytochemical and biological features of phyllanthus niruri and phyllanthus urinaria harvested at different growth stages revealed by h-1 nmr-based metabolomics
publisher	Elsevier
publishDate	2015
url	http://irep.iium.edu.my/45216/ http://irep.iium.edu.my/45216/ http://irep.iium.edu.my/45216/1/mediani_et_al._2015.pdf
first_indexed	2023-09-18T21:04:21Z
last_indexed	2023-09-18T21:04:21Z
_version_	1777410833199923200

Phytochemical and biological features of Phyllanthus niruri and Phyllanthus urinaria harvested at different growth stages revealed by H-1 NMR-based metabolomics

Similar Items