The potential of 3-dimensional construct engineered from poly(lactic-co-glycolic acid)/fibrin hybrid scaffold seeded with bone marrow mesenchymal stem cells for in vitro cartilage tissue engineering

Articular cartilage is well known for its simple uniqueness of avascular and aneural structure that has limited capacity to heal itself when injured. The use of three dimensional construct in tissue engineering holds great potential in regenerating cartilage defects. This study evaluated the in vitr...

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Main Authors:	Abdul Rahman, Rozlin, Mohd Shukri, Norhamiza, Md Nazir, Noorhidayah, Ahmad Radzi, Muhammad Aa'zamuddin, Zulkifly, Ahmad Hafiz, Che Ahmad, Aminudin, Hashi, Abdurezak Abdulahi, Abdul Rahman, Suzanah, Sha'ban, Munirah
Format:	Article
Language:	English English
Published:	Elsevier 2015
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/45264/ http://irep.iium.edu.my/45264/ http://irep.iium.edu.my/45264/ http://irep.iium.edu.my/45264/1/rozlin_et_al_2015_tissue_and_cell.pdf http://irep.iium.edu.my/45264/4/45264-The%20potential%20of%203-dimensional%20construct%20engineered%20from%20poly_SCOPUS.pdf

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recordtype	eprints
spelling	iium-452642017-08-02T01:38:06Z http://irep.iium.edu.my/45264/ The potential of 3-dimensional construct engineered from poly(lactic-co-glycolic acid)/fibrin hybrid scaffold seeded with bone marrow mesenchymal stem cells for in vitro cartilage tissue engineering Abdul Rahman, Rozlin Mohd Shukri, Norhamiza Md Nazir, Noorhidayah Ahmad Radzi, Muhammad Aa'zamuddin Zulkifly, Ahmad Hafiz Che Ahmad, Aminudin Hashi, Abdurezak Abdulahi Abdul Rahman, Suzanah Sha'ban, Munirah R Medicine (General) Articular cartilage is well known for its simple uniqueness of avascular and aneural structure that has limited capacity to heal itself when injured. The use of three dimensional construct in tissue engineering holds great potential in regenerating cartilage defects. This study evaluated the in vitro cartilaginous tissue formation using rabbit's bone marrow mesenchymal stem cells (BMSCs)-seeded onto poly(lactic-co-glycolic acid) PLGA/fibrin and PLGA scaffolds. The in vitro cartilaginous engineered constructs were evaluated by gross inspection, histology, cell proliferation, gene expression and sulphated glycosaminoglycan (sGAG) production at week 1, 2 and 3. After 3 weeks of culture, the PLGA/fibrin construct demonstrated gross features similar to the native tissue with smooth, firm and glistening appearance, superior histoarchitectural and better cartilaginous extracellular matrix compound in concert with the positive glycosaminoglycan accumulation on Alcian blue. Significantly higher cell proliferation in PLGA/fibrin construct was noted at day-7, day-14 and day-21 (p < 0.05 respectively). Both constructs expressed the accumulation of collagen type II, collagen type IX, aggrecan and sox9, showed down-regulation of collagen type I as well as produced relative sGAG content with PLGA/fibrin construct exhibited better gene expression in all profiles and showed significantly higher relative sGAG content at each time point (p < 0.05). This study suggested that with optimum in vitro manipulation, PLGA/fibrin when seeded with pluripotent non-committed BMSCs has the capability to differentiate into chondrogenic lineage and may serve as a prospective construct to be developed as functional tissue engineered cartilage. Elsevier 2015-08 Article PeerReviewed application/pdf en http://irep.iium.edu.my/45264/1/rozlin_et_al_2015_tissue_and_cell.pdf application/pdf en http://irep.iium.edu.my/45264/4/45264-The%20potential%20of%203-dimensional%20construct%20engineered%20from%20poly_SCOPUS.pdf Abdul Rahman, Rozlin and Mohd Shukri, Norhamiza and Md Nazir, Noorhidayah and Ahmad Radzi, Muhammad Aa'zamuddin and Zulkifly, Ahmad Hafiz and Che Ahmad, Aminudin and Hashi, Abdurezak Abdulahi and Abdul Rahman, Suzanah and Sha'ban, Munirah (2015) The potential of 3-dimensional construct engineered from poly(lactic-co-glycolic acid)/fibrin hybrid scaffold seeded with bone marrow mesenchymal stem cells for in vitro cartilage tissue engineering. Tissue and Cell, 47 (4). pp. 420-430. ISSN 0040-8166 http://www.sciencedirect.com/science/article/pii/S0040816615000531 10.1016/j.tice.2015.06.001
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Abdul Rahman, Rozlin Mohd Shukri, Norhamiza Md Nazir, Noorhidayah Ahmad Radzi, Muhammad Aa'zamuddin Zulkifly, Ahmad Hafiz Che Ahmad, Aminudin Hashi, Abdurezak Abdulahi Abdul Rahman, Suzanah Sha'ban, Munirah The potential of 3-dimensional construct engineered from poly(lactic-co-glycolic acid)/fibrin hybrid scaffold seeded with bone marrow mesenchymal stem cells for in vitro cartilage tissue engineering
description	Articular cartilage is well known for its simple uniqueness of avascular and aneural structure that has limited capacity to heal itself when injured. The use of three dimensional construct in tissue engineering holds great potential in regenerating cartilage defects. This study evaluated the in vitro cartilaginous tissue formation using rabbit's bone marrow mesenchymal stem cells (BMSCs)-seeded onto poly(lactic-co-glycolic acid) PLGA/fibrin and PLGA scaffolds. The in vitro cartilaginous engineered constructs were evaluated by gross inspection, histology, cell proliferation, gene expression and sulphated glycosaminoglycan (sGAG) production at week 1, 2 and 3. After 3 weeks of culture, the PLGA/fibrin construct demonstrated gross features similar to the native tissue with smooth, firm and glistening appearance, superior histoarchitectural and better cartilaginous extracellular matrix compound in concert with the positive glycosaminoglycan accumulation on Alcian blue. Significantly higher cell proliferation in PLGA/fibrin construct was noted at day-7, day-14 and day-21 (p < 0.05 respectively). Both constructs expressed the accumulation of collagen type II, collagen type IX, aggrecan and sox9, showed down-regulation of collagen type I as well as produced relative sGAG content with PLGA/fibrin construct exhibited better gene expression in all profiles and showed significantly higher relative sGAG content at each time point (p < 0.05). This study suggested that with optimum in vitro manipulation, PLGA/fibrin when seeded with pluripotent non-committed BMSCs has the capability to differentiate into chondrogenic lineage and may serve as a prospective construct to be developed as functional tissue engineered cartilage.
format	Article
author	Abdul Rahman, Rozlin Mohd Shukri, Norhamiza Md Nazir, Noorhidayah Ahmad Radzi, Muhammad Aa'zamuddin Zulkifly, Ahmad Hafiz Che Ahmad, Aminudin Hashi, Abdurezak Abdulahi Abdul Rahman, Suzanah Sha'ban, Munirah
author_facet	Abdul Rahman, Rozlin Mohd Shukri, Norhamiza Md Nazir, Noorhidayah Ahmad Radzi, Muhammad Aa'zamuddin Zulkifly, Ahmad Hafiz Che Ahmad, Aminudin Hashi, Abdurezak Abdulahi Abdul Rahman, Suzanah Sha'ban, Munirah
author_sort	Abdul Rahman, Rozlin
title	The potential of 3-dimensional construct engineered from poly(lactic-co-glycolic acid)/fibrin hybrid scaffold seeded with bone marrow mesenchymal stem cells for in vitro cartilage tissue engineering
title_short	The potential of 3-dimensional construct engineered from poly(lactic-co-glycolic acid)/fibrin hybrid scaffold seeded with bone marrow mesenchymal stem cells for in vitro cartilage tissue engineering
title_full	The potential of 3-dimensional construct engineered from poly(lactic-co-glycolic acid)/fibrin hybrid scaffold seeded with bone marrow mesenchymal stem cells for in vitro cartilage tissue engineering
title_fullStr	The potential of 3-dimensional construct engineered from poly(lactic-co-glycolic acid)/fibrin hybrid scaffold seeded with bone marrow mesenchymal stem cells for in vitro cartilage tissue engineering
title_full_unstemmed	The potential of 3-dimensional construct engineered from poly(lactic-co-glycolic acid)/fibrin hybrid scaffold seeded with bone marrow mesenchymal stem cells for in vitro cartilage tissue engineering
title_sort	potential of 3-dimensional construct engineered from poly(lactic-co-glycolic acid)/fibrin hybrid scaffold seeded with bone marrow mesenchymal stem cells for in vitro cartilage tissue engineering
publisher	Elsevier
publishDate	2015
url	http://irep.iium.edu.my/45264/ http://irep.iium.edu.my/45264/ http://irep.iium.edu.my/45264/ http://irep.iium.edu.my/45264/1/rozlin_et_al_2015_tissue_and_cell.pdf http://irep.iium.edu.my/45264/4/45264-The%20potential%20of%203-dimensional%20construct%20engineered%20from%20poly_SCOPUS.pdf
first_indexed	2023-09-18T21:04:26Z
last_indexed	2023-09-18T21:04:26Z
_version_	1777410838458531840

The potential of 3-dimensional construct engineered from poly(lactic-co-glycolic acid)/fibrin hybrid scaffold seeded with bone marrow mesenchymal stem cells for in vitro cartilage tissue engineering

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