Evaluation of three-dimensional construct engineered from poly(lactic-co-glycolic acid)/fibrin hybrid scaffold seeded with rabbit bone marrow Mesenchymal stem cells for osteochondral defect repair
Damage and degeneration of articular cartilage is a major health predicament. Articular cartilage has poor repair capacity due to its avascular and aneural properties, low metabolic rate and has relatively few cells. Left untreated, it may cause serious disability and loss of function. Therefore, th...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2015
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/45265/ http://irep.iium.edu.my/45265/1/45265.pdf |
| Summary: | Damage and degeneration of articular cartilage is a major health predicament. Articular cartilage has poor repair capacity due to its avascular and aneural properties, low metabolic rate and has relatively few cells. Left untreated, it may cause serious disability and loss of function. Therefore, the field of cartilage tissue engineering which aims to restore, repair and regenerate injured articular cartilage has evoked numerous interest for improving cartilage functionality. This study investigated the ability of autologous implantation approach using the three dimensional (3D) constructs engineered from bone marrow mesenchymal stem cells (BMSCs) seeded on poly(lactic-co-glycolic acid) (PLGA) with or without fibrin as cells carrier for the repair of osteochondral defect in rabbit model. With the approval of the International Islamic University Malaysia Research Ethics Committee, the engineered 3D constructs – PLGA/Fibrin/BMSCs and PLGA/BMSCs – were cultured for 3 weeks in vitro and implanted autologously to the osteochondral defect created in the rabbit knee. The in vivo constructs were harvested and evaluated by means of gross observation, histology assessment, gene expression study, sulphated glycosaminoglycan (sGAG) production assay and biomechanical evaluation at 6 and 12 weeks post implantation. The results showed that the osteochondral defects treated with the PLGA/Fibrin/BMSCs constructs exhibited better repairment, more cartilaginous extracellular matrix, higher sGAG production, superior compressive strength and greater expression of chondrogenic marker genes than the PLGA/BMSCs group. This study suggested that the PLGA/Fibrin/BMSCs has the potential to treat osteochondral defect and may be presented as a viable therapeutic option for those who would be in need from the life-extending benefits of tissue replacement or repair. |
|---|