Evaluation of three-dimensional construct engineered from poly(lactic-co-glycolic acid)/fibrin hybrid scaffold seeded with rabbit bone marrow Mesenchymal stem cells for osteochondral defect repair

Damage and degeneration of articular cartilage is a major health predicament. Articular cartilage has poor repair capacity due to its avascular and aneural properties, low metabolic rate and has relatively few cells. Left untreated, it may cause serious disability and loss of function. Therefore, th...

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Main Authors:	Abdul Rahman, Rozlin, Mohamad Sukri, Norhamiza, Md Nazir, Noorhidayah, Ahmad Radzi, Muhammad Aa'zamuddin, Zulkifly, Ahmad Hafiz, Che Ahmad, Aminuddin, Abdul Rahman, Suzanah, Sha'ban, Munirah
Format:	Conference or Workshop Item
Language:	English
Published:	2015
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/45265/ http://irep.iium.edu.my/45265/1/45265.pdf

id	iium-45265
recordtype	eprints
spelling	iium-452652018-02-26T10:21:41Z http://irep.iium.edu.my/45265/ Evaluation of three-dimensional construct engineered from poly(lactic-co-glycolic acid)/fibrin hybrid scaffold seeded with rabbit bone marrow Mesenchymal stem cells for osteochondral defect repair Abdul Rahman, Rozlin Mohamad Sukri, Norhamiza Md Nazir, Noorhidayah Ahmad Radzi, Muhammad Aa'zamuddin Zulkifly, Ahmad Hafiz Che Ahmad, Aminuddin Abdul Rahman, Suzanah Sha'ban, Munirah R Medicine (General) Damage and degeneration of articular cartilage is a major health predicament. Articular cartilage has poor repair capacity due to its avascular and aneural properties, low metabolic rate and has relatively few cells. Left untreated, it may cause serious disability and loss of function. Therefore, the field of cartilage tissue engineering which aims to restore, repair and regenerate injured articular cartilage has evoked numerous interest for improving cartilage functionality. This study investigated the ability of autologous implantation approach using the three dimensional (3D) constructs engineered from bone marrow mesenchymal stem cells (BMSCs) seeded on poly(lactic-co-glycolic acid) (PLGA) with or without fibrin as cells carrier for the repair of osteochondral defect in rabbit model. With the approval of the International Islamic University Malaysia Research Ethics Committee, the engineered 3D constructs – PLGA/Fibrin/BMSCs and PLGA/BMSCs – were cultured for 3 weeks in vitro and implanted autologously to the osteochondral defect created in the rabbit knee. The in vivo constructs were harvested and evaluated by means of gross observation, histology assessment, gene expression study, sulphated glycosaminoglycan (sGAG) production assay and biomechanical evaluation at 6 and 12 weeks post implantation. The results showed that the osteochondral defects treated with the PLGA/Fibrin/BMSCs constructs exhibited better repairment, more cartilaginous extracellular matrix, higher sGAG production, superior compressive strength and greater expression of chondrogenic marker genes than the PLGA/BMSCs group. This study suggested that the PLGA/Fibrin/BMSCs has the potential to treat osteochondral defect and may be presented as a viable therapeutic option for those who would be in need from the life-extending benefits of tissue replacement or repair. 2015-08 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/45265/1/45265.pdf Abdul Rahman, Rozlin and Mohamad Sukri, Norhamiza and Md Nazir, Noorhidayah and Ahmad Radzi, Muhammad Aa'zamuddin and Zulkifly, Ahmad Hafiz and Che Ahmad, Aminuddin and Abdul Rahman, Suzanah and Sha'ban, Munirah (2015) Evaluation of three-dimensional construct engineered from poly(lactic-co-glycolic acid)/fibrin hybrid scaffold seeded with rabbit bone marrow Mesenchymal stem cells for osteochondral defect repair. In: International Conference on Advancement in Science and Technology (iCAST 2015) - ‘Frontiers in Biotechnology’, 10th-12th August 2015, Impiana Resort Cherating, Pahang, Malaysia.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Abdul Rahman, Rozlin Mohamad Sukri, Norhamiza Md Nazir, Noorhidayah Ahmad Radzi, Muhammad Aa'zamuddin Zulkifly, Ahmad Hafiz Che Ahmad, Aminuddin Abdul Rahman, Suzanah Sha'ban, Munirah Evaluation of three-dimensional construct engineered from poly(lactic-co-glycolic acid)/fibrin hybrid scaffold seeded with rabbit bone marrow Mesenchymal stem cells for osteochondral defect repair
description	Damage and degeneration of articular cartilage is a major health predicament. Articular cartilage has poor repair capacity due to its avascular and aneural properties, low metabolic rate and has relatively few cells. Left untreated, it may cause serious disability and loss of function. Therefore, the field of cartilage tissue engineering which aims to restore, repair and regenerate injured articular cartilage has evoked numerous interest for improving cartilage functionality. This study investigated the ability of autologous implantation approach using the three dimensional (3D) constructs engineered from bone marrow mesenchymal stem cells (BMSCs) seeded on poly(lactic-co-glycolic acid) (PLGA) with or without fibrin as cells carrier for the repair of osteochondral defect in rabbit model. With the approval of the International Islamic University Malaysia Research Ethics Committee, the engineered 3D constructs – PLGA/Fibrin/BMSCs and PLGA/BMSCs – were cultured for 3 weeks in vitro and implanted autologously to the osteochondral defect created in the rabbit knee. The in vivo constructs were harvested and evaluated by means of gross observation, histology assessment, gene expression study, sulphated glycosaminoglycan (sGAG) production assay and biomechanical evaluation at 6 and 12 weeks post implantation. The results showed that the osteochondral defects treated with the PLGA/Fibrin/BMSCs constructs exhibited better repairment, more cartilaginous extracellular matrix, higher sGAG production, superior compressive strength and greater expression of chondrogenic marker genes than the PLGA/BMSCs group. This study suggested that the PLGA/Fibrin/BMSCs has the potential to treat osteochondral defect and may be presented as a viable therapeutic option for those who would be in need from the life-extending benefits of tissue replacement or repair.
format	Conference or Workshop Item
author	Abdul Rahman, Rozlin Mohamad Sukri, Norhamiza Md Nazir, Noorhidayah Ahmad Radzi, Muhammad Aa'zamuddin Zulkifly, Ahmad Hafiz Che Ahmad, Aminuddin Abdul Rahman, Suzanah Sha'ban, Munirah
author_facet	Abdul Rahman, Rozlin Mohamad Sukri, Norhamiza Md Nazir, Noorhidayah Ahmad Radzi, Muhammad Aa'zamuddin Zulkifly, Ahmad Hafiz Che Ahmad, Aminuddin Abdul Rahman, Suzanah Sha'ban, Munirah
author_sort	Abdul Rahman, Rozlin
title	Evaluation of three-dimensional construct engineered from poly(lactic-co-glycolic acid)/fibrin hybrid scaffold seeded with rabbit bone marrow Mesenchymal stem cells for osteochondral defect repair
title_short	Evaluation of three-dimensional construct engineered from poly(lactic-co-glycolic acid)/fibrin hybrid scaffold seeded with rabbit bone marrow Mesenchymal stem cells for osteochondral defect repair
title_full	Evaluation of three-dimensional construct engineered from poly(lactic-co-glycolic acid)/fibrin hybrid scaffold seeded with rabbit bone marrow Mesenchymal stem cells for osteochondral defect repair
title_fullStr	Evaluation of three-dimensional construct engineered from poly(lactic-co-glycolic acid)/fibrin hybrid scaffold seeded with rabbit bone marrow Mesenchymal stem cells for osteochondral defect repair
title_full_unstemmed	Evaluation of three-dimensional construct engineered from poly(lactic-co-glycolic acid)/fibrin hybrid scaffold seeded with rabbit bone marrow Mesenchymal stem cells for osteochondral defect repair
title_sort	evaluation of three-dimensional construct engineered from poly(lactic-co-glycolic acid)/fibrin hybrid scaffold seeded with rabbit bone marrow mesenchymal stem cells for osteochondral defect repair
publishDate	2015
url	http://irep.iium.edu.my/45265/ http://irep.iium.edu.my/45265/1/45265.pdf
first_indexed	2023-09-18T21:04:26Z
last_indexed	2023-09-18T21:04:26Z
_version_	1777410838604283904

Evaluation of three-dimensional construct engineered from poly(lactic-co-glycolic acid)/fibrin hybrid scaffold seeded with rabbit bone marrow Mesenchymal stem cells for osteochondral defect repair

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