Pathogenicity of a microsporidium isolate from the diamondback moth against noctuid moths: characterization and implications for microbiological pest management

Background: Due to problems with chemical control, there is increasing interest in the use of microsporidia for control of lepidopteran pests. However, there have been few studies to evaluate the susceptibility of exotic species to microsporidia from indigenous Lepidoptera. Methodology/Principal...

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Main Authors:	Abd Ghani, Idris, Dieng, Hamady, Abu Hassan, Zainal Abidin, Ramli, Norazsida, Kermani, Nadia, Satho, Tomomitsu, Ahmad, Hamdan, Abang, Fatimah, Fukumitsu, Yuki, Ahmad, Abu Hassan
Format:	Article
Language:	English
Published:	The Public Library of Science (PLoS) 2013
Subjects:	QL Zoology
Online Access:	http://irep.iium.edu.my/45279/ http://irep.iium.edu.my/45279/ http://irep.iium.edu.my/45279/1/publication_1_plos_one_2013.pdf

id	iium-45279
recordtype	eprints
spelling	iium-452792015-10-21T07:16:22Z http://irep.iium.edu.my/45279/ Pathogenicity of a microsporidium isolate from the diamondback moth against noctuid moths: characterization and implications for microbiological pest management Abd Ghani, Idris Dieng, Hamady Abu Hassan, Zainal Abidin Ramli, Norazsida Kermani, Nadia Satho, Tomomitsu Ahmad, Hamdan Abang, Fatimah Fukumitsu, Yuki Ahmad, Abu Hassan QL Zoology Background: Due to problems with chemical control, there is increasing interest in the use of microsporidia for control of lepidopteran pests. However, there have been few studies to evaluate the susceptibility of exotic species to microsporidia from indigenous Lepidoptera. Methodology/Principal Findings: We investigated some biological characteristics of the microsporidian parasite isolated from wild Plutella xylostella (PX) and evaluated its pathogenicity on the laboratory responses of sympatric invasive and resident noctuid moths. There were significant differences in spore size and morphology between PX and Spodoptera litura (SL) isolates. Spores of PX isolate were ovocylindrical, while those of SL were oval. PX spores were 1.05 times longer than those of SL, which in turn were 1.49 times wider than those of the PX. The timing of infection peaks was much shorter in SL and resulted in earlier larval death. There were no noticeable differences in amplicon size (two DNA fragments were each about 1200 base pairs in length). Phylogenetic analysis revealed that the small subunit (SSU) rRNA gene sequences of the two isolates shared a clade with Nosema/Vairimorpha sequences. The absence of octospores in infected spodopteran tissues suggested that PX and SL spores are closely related to Nosema plutellae and N. bombycis, respectively. Both SL and S. exigua (SE) exhibited susceptibility to the PX isolate infection, but showed different infection patterns. Tissular infection was more diverse in the former and resulted in much greater spore production and larval mortality. Microsporidium-infected larvae pupated among both infected and control larvae, but adult emergence occurred only in the second group. Conclusion/Significance: The PX isolate infection prevented completion of development of most leafworm and beet armyworm larvae. The ability of the microsporidian isolate to severely infect and kill larvae of both native and introduced spodopterans makes it a valuable candidate for biocontrol against lepidopteran pests. The Public Library of Science (PLoS) 2013-12-11 Article PeerReviewed application/pdf en http://irep.iium.edu.my/45279/1/publication_1_plos_one_2013.pdf Abd Ghani, Idris and Dieng, Hamady and Abu Hassan, Zainal Abidin and Ramli, Norazsida and Kermani, Nadia and Satho, Tomomitsu and Ahmad, Hamdan and Abang, Fatimah and Fukumitsu, Yuki and Ahmad, Abu Hassan (2013) Pathogenicity of a microsporidium isolate from the diamondback moth against noctuid moths: characterization and implications for microbiological pest management. PLoS ONE, 8 (12). e81642-1-e81642-10. ISSN 1932-6203 http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0081642
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QL Zoology
spellingShingle	QL Zoology Abd Ghani, Idris Dieng, Hamady Abu Hassan, Zainal Abidin Ramli, Norazsida Kermani, Nadia Satho, Tomomitsu Ahmad, Hamdan Abang, Fatimah Fukumitsu, Yuki Ahmad, Abu Hassan Pathogenicity of a microsporidium isolate from the diamondback moth against noctuid moths: characterization and implications for microbiological pest management
description	Background: Due to problems with chemical control, there is increasing interest in the use of microsporidia for control of lepidopteran pests. However, there have been few studies to evaluate the susceptibility of exotic species to microsporidia from indigenous Lepidoptera. Methodology/Principal Findings: We investigated some biological characteristics of the microsporidian parasite isolated from wild Plutella xylostella (PX) and evaluated its pathogenicity on the laboratory responses of sympatric invasive and resident noctuid moths. There were significant differences in spore size and morphology between PX and Spodoptera litura (SL) isolates. Spores of PX isolate were ovocylindrical, while those of SL were oval. PX spores were 1.05 times longer than those of SL, which in turn were 1.49 times wider than those of the PX. The timing of infection peaks was much shorter in SL and resulted in earlier larval death. There were no noticeable differences in amplicon size (two DNA fragments were each about 1200 base pairs in length). Phylogenetic analysis revealed that the small subunit (SSU) rRNA gene sequences of the two isolates shared a clade with Nosema/Vairimorpha sequences. The absence of octospores in infected spodopteran tissues suggested that PX and SL spores are closely related to Nosema plutellae and N. bombycis, respectively. Both SL and S. exigua (SE) exhibited susceptibility to the PX isolate infection, but showed different infection patterns. Tissular infection was more diverse in the former and resulted in much greater spore production and larval mortality. Microsporidium-infected larvae pupated among both infected and control larvae, but adult emergence occurred only in the second group. Conclusion/Significance: The PX isolate infection prevented completion of development of most leafworm and beet armyworm larvae. The ability of the microsporidian isolate to severely infect and kill larvae of both native and introduced spodopterans makes it a valuable candidate for biocontrol against lepidopteran pests.
format	Article
author	Abd Ghani, Idris Dieng, Hamady Abu Hassan, Zainal Abidin Ramli, Norazsida Kermani, Nadia Satho, Tomomitsu Ahmad, Hamdan Abang, Fatimah Fukumitsu, Yuki Ahmad, Abu Hassan
author_facet	Abd Ghani, Idris Dieng, Hamady Abu Hassan, Zainal Abidin Ramli, Norazsida Kermani, Nadia Satho, Tomomitsu Ahmad, Hamdan Abang, Fatimah Fukumitsu, Yuki Ahmad, Abu Hassan
author_sort	Abd Ghani, Idris
title	Pathogenicity of a microsporidium isolate from the diamondback moth against noctuid moths: characterization and implications for microbiological pest management
title_short	Pathogenicity of a microsporidium isolate from the diamondback moth against noctuid moths: characterization and implications for microbiological pest management
title_full	Pathogenicity of a microsporidium isolate from the diamondback moth against noctuid moths: characterization and implications for microbiological pest management
title_fullStr	Pathogenicity of a microsporidium isolate from the diamondback moth against noctuid moths: characterization and implications for microbiological pest management
title_full_unstemmed	Pathogenicity of a microsporidium isolate from the diamondback moth against noctuid moths: characterization and implications for microbiological pest management
title_sort	pathogenicity of a microsporidium isolate from the diamondback moth against noctuid moths: characterization and implications for microbiological pest management
publisher	The Public Library of Science (PLoS)
publishDate	2013
url	http://irep.iium.edu.my/45279/ http://irep.iium.edu.my/45279/ http://irep.iium.edu.my/45279/1/publication_1_plos_one_2013.pdf
first_indexed	2023-09-18T21:04:27Z
last_indexed	2023-09-18T21:04:27Z
_version_	1777410840207556608

Pathogenicity of a microsporidium isolate from the diamondback moth against noctuid moths: characterization and implications for microbiological pest management

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