Scheme of obtaining ß-carotene standard from pumpkin (Cucurbita moschata) flesh

Characterization and quantification of carotenoids composition is complicated, costly and time-consuming. The accuracy and the reliability of the data depend solely on the standards and HPLC but a major constraint is acquiring and maintaining pure standards. Carotenoid standards are commercially...

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Main Authors:	Shahidan, Norshazila, Othman, Rashidi, Jaswir, Irwandi, Hashim, Yumi Zuhanis Has-Yun
Format:	Conference or Workshop Item
Language:	English
Published:	2011
Subjects:	Q Science (General) QD Chemistry S Agriculture (General) SB Plant culture
Online Access:	http://irep.iium.edu.my/4562/ http://irep.iium.edu.my/4562/ http://irep.iium.edu.my/4562/4/Pages_from_RASHIDI_IREP5edit.pdf

id	iium-4562
recordtype	eprints
spelling	iium-45622012-01-19T04:01:11Z http://irep.iium.edu.my/4562/ Scheme of obtaining ß-carotene standard from pumpkin (Cucurbita moschata) flesh Shahidan, Norshazila Othman, Rashidi Jaswir, Irwandi Hashim, Yumi Zuhanis Has-Yun Q Science (General) QD Chemistry S Agriculture (General) SB Plant culture Characterization and quantification of carotenoids composition is complicated, costly and time-consuming. The accuracy and the reliability of the data depend solely on the standards and HPLC but a major constraint is acquiring and maintaining pure standards. Carotenoid standards are commercially available but they are expensive and are prone to isomerization and oxidation. Thus the purpose of this study is to establish analytical method for isolating beta-carotene by open column chromatography from pumpkin or Cucurbita moschata to be used as one of the carotenoid standards for determination of total and individual carotenoids. Pumpkin with orange flesh has been chosen due to the non-seasonal and abundance all year round. The study on the scheme of obtaining beta-carotene standard from pumpkin flesh demonstrated that the purity of beta-carotene standard evaluated by HPLC was 97.95%. The standard curves with five different concentrations for betacarotene in triplicate were constructed by plotting the area against the concentration. The coefficient of correlation was 0.9990. This study established that pumpkin can be a reliable source of beta-carotene standard which is cheap and can provide a constant supply throughout the year. 2011-05 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/4562/4/Pages_from_RASHIDI_IREP5edit.pdf Shahidan, Norshazila and Othman, Rashidi and Jaswir, Irwandi and Hashim, Yumi Zuhanis Has-Yun (2011) Scheme of obtaining ß-carotene standard from pumpkin (Cucurbita moschata) flesh. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May 2011, The Legend Hotel, Kuala Lumpur. http://www.iium.edu.my/icbioe/2011/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General) QD Chemistry S Agriculture (General) SB Plant culture
spellingShingle	Q Science (General) QD Chemistry S Agriculture (General) SB Plant culture Shahidan, Norshazila Othman, Rashidi Jaswir, Irwandi Hashim, Yumi Zuhanis Has-Yun Scheme of obtaining ß-carotene standard from pumpkin (Cucurbita moschata) flesh
description	Characterization and quantification of carotenoids composition is complicated, costly and time-consuming. The accuracy and the reliability of the data depend solely on the standards and HPLC but a major constraint is acquiring and maintaining pure standards. Carotenoid standards are commercially available but they are expensive and are prone to isomerization and oxidation. Thus the purpose of this study is to establish analytical method for isolating beta-carotene by open column chromatography from pumpkin or Cucurbita moschata to be used as one of the carotenoid standards for determination of total and individual carotenoids. Pumpkin with orange flesh has been chosen due to the non-seasonal and abundance all year round. The study on the scheme of obtaining beta-carotene standard from pumpkin flesh demonstrated that the purity of beta-carotene standard evaluated by HPLC was 97.95%. The standard curves with five different concentrations for betacarotene in triplicate were constructed by plotting the area against the concentration. The coefficient of correlation was 0.9990. This study established that pumpkin can be a reliable source of beta-carotene standard which is cheap and can provide a constant supply throughout the year.
format	Conference or Workshop Item
author	Shahidan, Norshazila Othman, Rashidi Jaswir, Irwandi Hashim, Yumi Zuhanis Has-Yun
author_facet	Shahidan, Norshazila Othman, Rashidi Jaswir, Irwandi Hashim, Yumi Zuhanis Has-Yun
author_sort	Shahidan, Norshazila
title	Scheme of obtaining ß-carotene standard from pumpkin (Cucurbita moschata) flesh
title_short	Scheme of obtaining ß-carotene standard from pumpkin (Cucurbita moschata) flesh
title_full	Scheme of obtaining ß-carotene standard from pumpkin (Cucurbita moschata) flesh
title_fullStr	Scheme of obtaining ß-carotene standard from pumpkin (Cucurbita moschata) flesh
title_full_unstemmed	Scheme of obtaining ß-carotene standard from pumpkin (Cucurbita moschata) flesh
title_sort	scheme of obtaining ß-carotene standard from pumpkin (cucurbita moschata) flesh
publishDate	2011
url	http://irep.iium.edu.my/4562/ http://irep.iium.edu.my/4562/ http://irep.iium.edu.my/4562/4/Pages_from_RASHIDI_IREP5edit.pdf
first_indexed	2023-09-18T20:12:49Z
last_indexed	2023-09-18T20:12:49Z
_version_	1777407591131906048

Scheme of obtaining ß-carotene standard from pumpkin (Cucurbita moschata) flesh

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