Encapsulated embryogenic callus of clitoria ternatea L. for regeneration and conservation
Encapsulated embryogenic callus of Clitoria ternatea L. were successfully created from leaf explants within 3 weeks after germination on Murashige and Skoog (MS) media. The seeds were initially washed with tap water and teepol, then the seeds were sterilised with 99% (v/v) sodium hypochlorite s...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
IACSIT Press
2016
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/45772/ http://irep.iium.edu.my/45772/ http://irep.iium.edu.my/45772/ http://irep.iium.edu.my/45772/1/PAPER_2.pdf |
| Summary: | Encapsulated embryogenic callus of Clitoria
ternatea L. were successfully created from leaf explants within 3
weeks after germination on Murashige and Skoog (MS) media.
The seeds were initially washed with tap water and teepol, then
the seeds were sterilised with 99% (v/v) sodium hypochlorite
solution for 1 minute and rinsed with distilled water three times.
In a laminar flow cabinet, the seeds were dipped in 70% (v/v)
ethanol for 1 minute and blotted with steriled tissue. The 3 mm2
leaf explants were encapsulated with 3% alginate (w/v) which
were suplemented with various concentrations (0.5-2.5 mg l-1
)
and combinations of NAA, BAP and adenine. The optimum
concentration for the formation of encapsulation matrix was
3.0% sodium alginate (NaC6H7O6
). Encapsulated beads were
soaked in 100 mM calcium chloride dehydrate (CaCl2
.2H2O)
solution for 30 minutes. No suitable beads were formed with low
concentration (1-2%) of sodium alginate. Within 10 minutes
soaking in calcium chloride dehydrate, clear and bead formation
with no definite shape was observed. While, within 20 minutes in
calcium chloride dehydrate, clear beads, solid and round in
shape was observed, however, inside the bead was still in liquid
condition. In the present study, the rate of germination of
synthetic seeds were slightly decreased from 100% to 77% after
60 days of storage at 4°C. Embryogenic tissue from leaf explants
of Clitoria ternatea was distinguished by double staining method
with bright red of acetocarmine. This technology is an
alternative and supplementary method for regeneration, mass
propagation and conservation of this medicinal, attractive
ornamental and also forage crop for future uses and
exploitation |
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