Functional characterization of recombinant bromelain
Stem bromelain is a plant protease with a number of industrial and therapeutic applications. This study investigated the functional properties of commercial bromelain and recombinant bromelain (expressed in E. coli BL 21-AI). In vitro cytotoxicity assays were conducted to evaluate the effect of b...
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2015
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| Online Access: | http://irep.iium.edu.my/45893/ http://irep.iium.edu.my/45893/1/oral_presentation-Azura.pdf |
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iium-458932016-05-23T02:10:55Z http://irep.iium.edu.my/45893/ Functional characterization of recombinant bromelain Amid, Azura Bala, Muntari Mohd. Salleh, Hamzah TP248.13 Biotechnology Stem bromelain is a plant protease with a number of industrial and therapeutic applications. This study investigated the functional properties of commercial bromelain and recombinant bromelain (expressed in E. coli BL 21-AI). In vitro cytotoxicity assays were conducted to evaluate the effect of both bromelains on tumour cell lines (murine melanoma and breast cancer MCF-7) and normal cells (Vero and Chinese hamster ovary) using Sulforhodamine B and MTT tests. Anti-inflammatory activities of the enzymes were also assessed on murine macrophage cell lines using Griess reagents and ELISA test. The beef tenderizing effects of both bromelains and in-situ localization of the recombinant bromelain were analysed using Transmission Electron Microscopy. The results obtained revealed that both bromelains were more cytotoxic to tumour cell lines (IC50 values ≈ 0.16-0.23mg/mL) than normal cell lines (IC50 values ≈ 0.27-0.51mg/mL). Moreover, the two enzymes had effectively inhibited the pro-inflammatory mediators’ production and thus, had good anti-inflammatory activity. Both enzymes were found to have comparative anti-cancer and anti-inflammatory properties. The expression of recombinant enzyme was discovered to occur in the cytoplasm of E. coli BL 21-AI and the purified enzyme efficiently degraded both actin and myosin of fresh beef. Hence, it served as a good meat tenderizer. 2015-11-17 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/45893/1/oral_presentation-Azura.pdf Amid, Azura and Bala, Muntari and Mohd. Salleh, Hamzah (2015) Functional characterization of recombinant bromelain. In: ASIAN Congress on Biotechnology 2015, 15th-19th Nov. 2015, Kuala Lumpur. |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
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IIUM Repository |
| collection |
Online Access |
| language |
English |
| topic |
TP248.13 Biotechnology |
| spellingShingle |
TP248.13 Biotechnology Amid, Azura Bala, Muntari Mohd. Salleh, Hamzah Functional characterization of recombinant bromelain |
| description |
Stem bromelain is a plant protease with a number of industrial and therapeutic
applications. This study investigated the functional properties of commercial bromelain
and recombinant bromelain (expressed in E. coli BL 21-AI). In vitro cytotoxicity assays
were conducted to evaluate the effect of both bromelains on tumour cell lines (murine
melanoma and breast cancer MCF-7) and normal cells (Vero and Chinese hamster
ovary) using Sulforhodamine B and MTT tests. Anti-inflammatory activities of the
enzymes were also assessed on murine macrophage cell lines using Griess reagents and
ELISA test. The beef tenderizing effects of both bromelains and in-situ localization of the
recombinant bromelain were analysed using Transmission Electron Microscopy. The
results obtained revealed that both bromelains were more cytotoxic to tumour cell lines
(IC50 values ≈ 0.16-0.23mg/mL) than normal cell lines (IC50 values ≈ 0.27-0.51mg/mL).
Moreover, the two enzymes had effectively inhibited the pro-inflammatory mediators’
production and thus, had good anti-inflammatory activity. Both enzymes were found to
have comparative anti-cancer and anti-inflammatory properties. The expression of
recombinant enzyme was discovered to occur in the cytoplasm of E. coli BL 21-AI and
the purified enzyme efficiently degraded both actin and myosin of fresh beef. Hence, it
served as a good meat tenderizer. |
| format |
Conference or Workshop Item |
| author |
Amid, Azura Bala, Muntari Mohd. Salleh, Hamzah |
| author_facet |
Amid, Azura Bala, Muntari Mohd. Salleh, Hamzah |
| author_sort |
Amid, Azura |
| title |
Functional characterization of recombinant bromelain |
| title_short |
Functional characterization of recombinant bromelain |
| title_full |
Functional characterization of recombinant bromelain |
| title_fullStr |
Functional characterization of recombinant bromelain |
| title_full_unstemmed |
Functional characterization of recombinant bromelain |
| title_sort |
functional characterization of recombinant bromelain |
| publishDate |
2015 |
| url |
http://irep.iium.edu.my/45893/ http://irep.iium.edu.my/45893/1/oral_presentation-Azura.pdf |
| first_indexed |
2023-09-18T21:05:19Z |
| last_indexed |
2023-09-18T21:05:19Z |
| _version_ |
1777410894335049728 |