Development of LC/MS-MS method for analysis of polyphenols in Malaysian traditional vegetables

Development of LC/MS-MS method for analysis of polyphenols in Malaysian traditional vegetables

Development and validation of an LC-MS/MS method suitable for the simultaneous quantification of phenolic acids and flavonoids is described. Analytes were separated on a C6-phenyl column, analysed using negative ion APCI selected reaction monitoring. The method is capable of analysing 14 compounds...

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Main Authors: Haron, Normah, Arcot, Jayashree, Bucknall, Martin
Format: Conference or Workshop Item
Language:English
Published: 2015
Subjects:
Q Science (General)
S Agriculture (General)
Online Access:http://irep.iium.edu.my/45944/
http://irep.iium.edu.my/45944/4/45944.pdf
id iium-45944
recordtype eprints
spelling iium-459442017-12-05T08:03:36Z http://irep.iium.edu.my/45944/ Development of LC/MS-MS method for analysis of polyphenols in Malaysian traditional vegetables Haron, Normah Arcot, Jayashree Bucknall, Martin Q Science (General) S Agriculture (General) Development and validation of an LC-MS/MS method suitable for the simultaneous quantification of phenolic acids and flavonoids is described. Analytes were separated on a C6-phenyl column, analysed using negative ion APCI selected reaction monitoring. The method is capable of analysing 14 compounds (9 phenolic acids and 5 flavonoids) in 85 minutes and uses deuterium-labelled 4-hydroxy-3-methoxy-d3-cinnamic acid (ferulic acid-d3) and 13C15-quercetin as internal standards. The results demonstrated that the method developed was reliable, specific and robust. Thus the method was successfully applied to analyse phenolic compounds in 18 different types of traditional Malaysian vegetables (both raw and processed) with 3 different fractions of phenolic acids. Gallic, ferulic, vanillic and caffeic acids were the most dominant phenolic acids, while quercetin, apigenin, kaempferol and luteolin were the predominant flavonoids in the samples studied. 2015-11-15 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/45944/4/45944.pdf Haron, Normah and Arcot, Jayashree and Bucknall, Martin (2015) Development of LC/MS-MS method for analysis of polyphenols in Malaysian traditional vegetables. In: Asian Congress on Biotechnology 2015, 15th-19th November 2015, Kuala Lumpur. (Unpublished)
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic Q Science (General)
S Agriculture (General)
spellingShingle Q Science (General)
S Agriculture (General)
Haron, Normah
Arcot, Jayashree
Bucknall, Martin
Development of LC/MS-MS method for analysis of polyphenols in Malaysian traditional vegetables
description Development and validation of an LC-MS/MS method suitable for the simultaneous quantification of phenolic acids and flavonoids is described. Analytes were separated on a C6-phenyl column, analysed using negative ion APCI selected reaction monitoring. The method is capable of analysing 14 compounds (9 phenolic acids and 5 flavonoids) in 85 minutes and uses deuterium-labelled 4-hydroxy-3-methoxy-d3-cinnamic acid (ferulic acid-d3) and 13C15-quercetin as internal standards. The results demonstrated that the method developed was reliable, specific and robust. Thus the method was successfully applied to analyse phenolic compounds in 18 different types of traditional Malaysian vegetables (both raw and processed) with 3 different fractions of phenolic acids. Gallic, ferulic, vanillic and caffeic acids were the most dominant phenolic acids, while quercetin, apigenin, kaempferol and luteolin were the predominant flavonoids in the samples studied.
format Conference or Workshop Item
author Haron, Normah
Arcot, Jayashree
Bucknall, Martin
author_facet Haron, Normah
Arcot, Jayashree
Bucknall, Martin
author_sort Haron, Normah
title Development of LC/MS-MS method for analysis of polyphenols in Malaysian traditional vegetables
title_short Development of LC/MS-MS method for analysis of polyphenols in Malaysian traditional vegetables
title_full Development of LC/MS-MS method for analysis of polyphenols in Malaysian traditional vegetables
title_fullStr Development of LC/MS-MS method for analysis of polyphenols in Malaysian traditional vegetables
title_full_unstemmed Development of LC/MS-MS method for analysis of polyphenols in Malaysian traditional vegetables
title_sort development of lc/ms-ms method for analysis of polyphenols in malaysian traditional vegetables
publishDate 2015
url http://irep.iium.edu.my/45944/
http://irep.iium.edu.my/45944/4/45944.pdf
first_indexed 2023-09-18T21:05:23Z
last_indexed 2023-09-18T21:05:23Z
_version_ 1777410898258821120

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