Method development and validation using UV spectrophotometry for Nigella sativa oil microparticles quantification

Nigella sativa oil (NSO) has been exploited for medical purposes for many generations. The fabrication of microparticles containing NSO intended for sustained release was done to be used in treating osteomyelitis. Method in quantifying NSO using UV-spectroscopy was developed and validated. Linearity...

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Main Authors:	Harun Ismail, Ahmad Fahmi, Doolaanea, Abd Almonem, Mohamed, Farahidah, Mansor, Nur 'Izzati, Mohd Shafri, Mohd Affendi, Yusof, Fathin Athirah
Format:	Article
Language:	English
Published:	Open Science Publishers LLP Inc. 2015
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/46273/ http://irep.iium.edu.my/46273/ http://irep.iium.edu.my/46273/ http://irep.iium.edu.my/46273/1/Method_UV_Spectrophotometry_for_Nigella_sativa_Oil.pdf

id	iium-46273
recordtype	eprints
spelling	iium-462732017-02-14T03:00:42Z http://irep.iium.edu.my/46273/ Method development and validation using UV spectrophotometry for Nigella sativa oil microparticles quantification Harun Ismail, Ahmad Fahmi Doolaanea, Abd Almonem Mohamed, Farahidah Mansor, Nur 'Izzati Mohd Shafri, Mohd Affendi Yusof, Fathin Athirah RS Pharmacy and materia medica Nigella sativa oil (NSO) has been exploited for medical purposes for many generations. The fabrication of microparticles containing NSO intended for sustained release was done to be used in treating osteomyelitis. Method in quantifying NSO using UV-spectroscopy was developed and validated. Linearity shown a good correlation coefficient with the values higher than 0.995, both for actual and different analysts. The LOD and LOQ values were recorded to be 2.89 μg/mL and 8.75 μg/mL respectively. In addition, the highest %RSD values for the intermediate and repeatability studies were 0.970% and 0.445% which suggested the method was precise. The percentage recovery for 4 known concentrations gave the range between 98.16% to 99.39%, indicating the high accuracy of the method. The parameters analyzed in this study were in accordance with ICH Q2 (R1) guidelines. Open Science Publishers LLP Inc. 2015-09 Article NonPeerReviewed application/pdf en http://irep.iium.edu.my/46273/1/Method_UV_Spectrophotometry_for_Nigella_sativa_Oil.pdf Harun Ismail, Ahmad Fahmi and Doolaanea, Abd Almonem and Mohamed, Farahidah and Mansor, Nur 'Izzati and Mohd Shafri, Mohd Affendi and Yusof, Fathin Athirah (2015) Method development and validation using UV spectrophotometry for Nigella sativa oil microparticles quantification. Journal of Applied Pharmaceutical Science, 5 (9). 082-088. ISSN 2231-3354 http://www.japsonline.com/admin/php/uploads/1628_pdf.pdf 10.7324/JAPS.2015.50915
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Harun Ismail, Ahmad Fahmi Doolaanea, Abd Almonem Mohamed, Farahidah Mansor, Nur 'Izzati Mohd Shafri, Mohd Affendi Yusof, Fathin Athirah Method development and validation using UV spectrophotometry for Nigella sativa oil microparticles quantification
description	Nigella sativa oil (NSO) has been exploited for medical purposes for many generations. The fabrication of microparticles containing NSO intended for sustained release was done to be used in treating osteomyelitis. Method in quantifying NSO using UV-spectroscopy was developed and validated. Linearity shown a good correlation coefficient with the values higher than 0.995, both for actual and different analysts. The LOD and LOQ values were recorded to be 2.89 μg/mL and 8.75 μg/mL respectively. In addition, the highest %RSD values for the intermediate and repeatability studies were 0.970% and 0.445% which suggested the method was precise. The percentage recovery for 4 known concentrations gave the range between 98.16% to 99.39%, indicating the high accuracy of the method. The parameters analyzed in this study were in accordance with ICH Q2 (R1) guidelines.
format	Article
author	Harun Ismail, Ahmad Fahmi Doolaanea, Abd Almonem Mohamed, Farahidah Mansor, Nur 'Izzati Mohd Shafri, Mohd Affendi Yusof, Fathin Athirah
author_facet	Harun Ismail, Ahmad Fahmi Doolaanea, Abd Almonem Mohamed, Farahidah Mansor, Nur 'Izzati Mohd Shafri, Mohd Affendi Yusof, Fathin Athirah
author_sort	Harun Ismail, Ahmad Fahmi
title	Method development and validation using UV spectrophotometry for Nigella sativa oil microparticles quantification
title_short	Method development and validation using UV spectrophotometry for Nigella sativa oil microparticles quantification
title_full	Method development and validation using UV spectrophotometry for Nigella sativa oil microparticles quantification
title_fullStr	Method development and validation using UV spectrophotometry for Nigella sativa oil microparticles quantification
title_full_unstemmed	Method development and validation using UV spectrophotometry for Nigella sativa oil microparticles quantification
title_sort	method development and validation using uv spectrophotometry for nigella sativa oil microparticles quantification
publisher	Open Science Publishers LLP Inc.
publishDate	2015
url	http://irep.iium.edu.my/46273/ http://irep.iium.edu.my/46273/ http://irep.iium.edu.my/46273/ http://irep.iium.edu.my/46273/1/Method_UV_Spectrophotometry_for_Nigella_sativa_Oil.pdf
first_indexed	2023-09-18T21:05:53Z
last_indexed	2023-09-18T21:05:53Z
_version_	1777410929687789568

Method development and validation using UV spectrophotometry for Nigella sativa oil microparticles quantification

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