DNA amplification, optimization and cloning of several target genes from Burkholderia pseudomallei

Burkholderia pseudomallei is a saprophytic Gram-negative bacillus that is found in soil and surface water. It causes the disease melioidosis, which infect humans and animals. Melioidosis can be fatal in human, where it causes fever and commonly present with pneumonia, with or without septicaemia. Me...

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Main Authors:	Osman, Abdul Latif, Syed Abdullah, Syed Mohamed, Drahaman, Siti Marhamah, Raih, Mohd Firdaus, Muhamad Bunnori, Noraslinda, Mohamed Rehan, Aisyah
Format:	Article
Language:	English
Published:	Universiti Teknologi Malaysia 2015
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/46351/ http://irep.iium.edu.my/46351/ http://irep.iium.edu.my/46351/1/2015_JurnalTeknologi_Abdul_Latif_et_al_AISYAH_B._Pseudomallei.pdf

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spelling	iium-463512018-06-12T03:26:15Z http://irep.iium.edu.my/46351/ DNA amplification, optimization and cloning of several target genes from Burkholderia pseudomallei Osman, Abdul Latif Syed Abdullah, Syed Mohamed Drahaman, Siti Marhamah Raih, Mohd Firdaus Muhamad Bunnori, Noraslinda Mohamed Rehan, Aisyah QR Microbiology Burkholderia pseudomallei is a saprophytic Gram-negative bacillus that is found in soil and surface water. It causes the disease melioidosis, which infect humans and animals. Melioidosis can be fatal in human, where it causes fever and commonly present with pneumonia, with or without septicaemia. Melioidosis is primarily endemic in Southeast Asia and Northern Australia, but has also been found in the Middle East, China and South America. It is challenging to treat melioidosis, as it is intrinsically resistant to many antibiotics, and can cause latent infection. By characterizing identified protein targets from B. pseudomallei, we can gain fundamental knowledge on how this bacterium behaves, and thus provide us strategies to combat them. We report here the recent progress of DNA amplification and cloning of four target genes from B. pseudomallei strain D286 performed in Kulliyyah of Science, IIUM. Genomic DNA of B. pseudomallei strain D286 is obtained from School of Biosciences & Biotechnology, UKM. The four target genes; BPSL1612, BPSL1618, BPSL1691 and BPSL2054 were chosen from 52 hypothetical proteins predicted to be essential in B. pseudomallei by transposon-directed insertion site sequencing (TraDIS) technique (Moule et al., 2014). All four target genes were subjected to nested PCR amplification for subsequent GatewayTM cloning protocols, expression and purification studies. Universiti Teknologi Malaysia 2015-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/46351/1/2015_JurnalTeknologi_Abdul_Latif_et_al_AISYAH_B._Pseudomallei.pdf Osman, Abdul Latif and Syed Abdullah, Syed Mohamed and Drahaman, Siti Marhamah and Raih, Mohd Firdaus and Muhamad Bunnori, Noraslinda and Mohamed Rehan, Aisyah (2015) DNA amplification, optimization and cloning of several target genes from Burkholderia pseudomallei. Jurnal Teknologi, 77 (25). pp. 153-158. ISSN 2180–3722 (O), 0127–9696 (P) http://www.jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/6756
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Osman, Abdul Latif Syed Abdullah, Syed Mohamed Drahaman, Siti Marhamah Raih, Mohd Firdaus Muhamad Bunnori, Noraslinda Mohamed Rehan, Aisyah DNA amplification, optimization and cloning of several target genes from Burkholderia pseudomallei
description	Burkholderia pseudomallei is a saprophytic Gram-negative bacillus that is found in soil and surface water. It causes the disease melioidosis, which infect humans and animals. Melioidosis can be fatal in human, where it causes fever and commonly present with pneumonia, with or without septicaemia. Melioidosis is primarily endemic in Southeast Asia and Northern Australia, but has also been found in the Middle East, China and South America. It is challenging to treat melioidosis, as it is intrinsically resistant to many antibiotics, and can cause latent infection. By characterizing identified protein targets from B. pseudomallei, we can gain fundamental knowledge on how this bacterium behaves, and thus provide us strategies to combat them. We report here the recent progress of DNA amplification and cloning of four target genes from B. pseudomallei strain D286 performed in Kulliyyah of Science, IIUM. Genomic DNA of B. pseudomallei strain D286 is obtained from School of Biosciences & Biotechnology, UKM. The four target genes; BPSL1612, BPSL1618, BPSL1691 and BPSL2054 were chosen from 52 hypothetical proteins predicted to be essential in B. pseudomallei by transposon-directed insertion site sequencing (TraDIS) technique (Moule et al., 2014). All four target genes were subjected to nested PCR amplification for subsequent GatewayTM cloning protocols, expression and purification studies.
format	Article
author	Osman, Abdul Latif Syed Abdullah, Syed Mohamed Drahaman, Siti Marhamah Raih, Mohd Firdaus Muhamad Bunnori, Noraslinda Mohamed Rehan, Aisyah
author_facet	Osman, Abdul Latif Syed Abdullah, Syed Mohamed Drahaman, Siti Marhamah Raih, Mohd Firdaus Muhamad Bunnori, Noraslinda Mohamed Rehan, Aisyah
author_sort	Osman, Abdul Latif
title	DNA amplification, optimization and cloning of several target genes from Burkholderia pseudomallei
title_short	DNA amplification, optimization and cloning of several target genes from Burkholderia pseudomallei
title_full	DNA amplification, optimization and cloning of several target genes from Burkholderia pseudomallei
title_fullStr	DNA amplification, optimization and cloning of several target genes from Burkholderia pseudomallei
title_full_unstemmed	DNA amplification, optimization and cloning of several target genes from Burkholderia pseudomallei
title_sort	dna amplification, optimization and cloning of several target genes from burkholderia pseudomallei
publisher	Universiti Teknologi Malaysia
publishDate	2015
url	http://irep.iium.edu.my/46351/ http://irep.iium.edu.my/46351/ http://irep.iium.edu.my/46351/1/2015_JurnalTeknologi_Abdul_Latif_et_al_AISYAH_B._Pseudomallei.pdf
first_indexed	2023-09-18T21:05:59Z
last_indexed	2023-09-18T21:05:59Z
_version_	1777410936495144960

DNA amplification, optimization and cloning of several target genes from Burkholderia pseudomallei

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