Investigation on the difference expression of type IV collagen α1(IV)- α6(IV) chain mrna in normal fibroblast and in skin cell malignant melanoma

Collagen IV is the major basement membrane protein that influences adhesion, proliferation, and migration of cells. The collagen composed of a network chains a1 to a6. The characterization of this collagen IV will correlates the relationship of collagen gene expression and cancer. This is important...

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Main Authors:	Mohammad, Mardhiah, Mat Yusof, Afzan, Kaja Mohideen, Syahirah, Syed Bahrom, Sharifah Norbaizura, Roshdi, Ridhwan, Parman, Adibah
Format:	Article
Language:	English
Published:	Universiti Teknologi Malaysia 2015
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/46358/ http://irep.iium.edu.my/46358/ http://irep.iium.edu.my/46358/ http://irep.iium.edu.my/46358/1/6754-18543-1-SM_%281%29MAR.pdf

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spelling	iium-463582016-05-01T15:59:08Z http://irep.iium.edu.my/46358/ Investigation on the difference expression of type IV collagen α1(IV)- α6(IV) chain mrna in normal fibroblast and in skin cell malignant melanoma Mohammad, Mardhiah Mat Yusof, Afzan Kaja Mohideen, Syahirah Syed Bahrom, Sharifah Norbaizura Roshdi, Ridhwan Parman, Adibah Q Science (General) Collagen IV is the major basement membrane protein that influences adhesion, proliferation, and migration of cells. The collagen composed of a network chains a1 to a6. The characterization of this collagen IV will correlates the relationship of collagen gene expression and cancer. This is important in order to provide more detailed understanding of the expression of collagen in tumor cells. . The aim of this study is to determine the a1 to a6 (IV) mRNA expression in the cell lines obtained from skin and melanoma cell. To investigate the mRNA expression, the RNA was extracted from the fibroblast and melanoma (A375) cell lines. The RNA was subjected to reverse transcription and then synthesized. The mRNA expression levels were measured using real time PCR with related to internal control, GAPDH. The study identified that a1, a2, a4, a5 and a6 of the a1-a6 (IV) were expressed in skin fibroblast. This corresponds to the a1a1a2 and a5a5a6 networks. However, in melanoma cell lines the collagen IV a2, a4, a5 and a6 mRNA was observed in low level compared to a1 and this suggested that the tumor has affected the expression of collagen and basement membrane of the cell. Universiti Teknologi Malaysia 2015-10-15 Article PeerReviewed application/pdf en http://irep.iium.edu.my/46358/1/6754-18543-1-SM_%281%29MAR.pdf Mohammad, Mardhiah and Mat Yusof, Afzan and Kaja Mohideen, Syahirah and Syed Bahrom, Sharifah Norbaizura and Roshdi, Ridhwan and Parman, Adibah (2015) Investigation on the difference expression of type IV collagen α1(IV)- α6(IV) chain mrna in normal fibroblast and in skin cell malignant melanoma. Jurnal Teknologi, 77 (25). pp. 141-145. ISSN 0127–9696 E-ISSN 2180–3722 http://www.jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/6754 10.11113/jt.v77.6754
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Mohammad, Mardhiah Mat Yusof, Afzan Kaja Mohideen, Syahirah Syed Bahrom, Sharifah Norbaizura Roshdi, Ridhwan Parman, Adibah Investigation on the difference expression of type IV collagen α1(IV)- α6(IV) chain mrna in normal fibroblast and in skin cell malignant melanoma
description	Collagen IV is the major basement membrane protein that influences adhesion, proliferation, and migration of cells. The collagen composed of a network chains a1 to a6. The characterization of this collagen IV will correlates the relationship of collagen gene expression and cancer. This is important in order to provide more detailed understanding of the expression of collagen in tumor cells. . The aim of this study is to determine the a1 to a6 (IV) mRNA expression in the cell lines obtained from skin and melanoma cell. To investigate the mRNA expression, the RNA was extracted from the fibroblast and melanoma (A375) cell lines. The RNA was subjected to reverse transcription and then synthesized. The mRNA expression levels were measured using real time PCR with related to internal control, GAPDH. The study identified that a1, a2, a4, a5 and a6 of the a1-a6 (IV) were expressed in skin fibroblast. This corresponds to the a1a1a2 and a5a5a6 networks. However, in melanoma cell lines the collagen IV a2, a4, a5 and a6 mRNA was observed in low level compared to a1 and this suggested that the tumor has affected the expression of collagen and basement membrane of the cell.
format	Article
author	Mohammad, Mardhiah Mat Yusof, Afzan Kaja Mohideen, Syahirah Syed Bahrom, Sharifah Norbaizura Roshdi, Ridhwan Parman, Adibah
author_facet	Mohammad, Mardhiah Mat Yusof, Afzan Kaja Mohideen, Syahirah Syed Bahrom, Sharifah Norbaizura Roshdi, Ridhwan Parman, Adibah
author_sort	Mohammad, Mardhiah
title	Investigation on the difference expression of type IV collagen α1(IV)- α6(IV) chain mrna in normal fibroblast and in skin cell malignant melanoma
title_short	Investigation on the difference expression of type IV collagen α1(IV)- α6(IV) chain mrna in normal fibroblast and in skin cell malignant melanoma
title_full	Investigation on the difference expression of type IV collagen α1(IV)- α6(IV) chain mrna in normal fibroblast and in skin cell malignant melanoma
title_fullStr	Investigation on the difference expression of type IV collagen α1(IV)- α6(IV) chain mrna in normal fibroblast and in skin cell malignant melanoma
title_full_unstemmed	Investigation on the difference expression of type IV collagen α1(IV)- α6(IV) chain mrna in normal fibroblast and in skin cell malignant melanoma
title_sort	investigation on the difference expression of type iv collagen α1(iv)- α6(iv) chain mrna in normal fibroblast and in skin cell malignant melanoma
publisher	Universiti Teknologi Malaysia
publishDate	2015
url	http://irep.iium.edu.my/46358/ http://irep.iium.edu.my/46358/ http://irep.iium.edu.my/46358/ http://irep.iium.edu.my/46358/1/6754-18543-1-SM_%281%29MAR.pdf
first_indexed	2023-09-18T21:06:00Z
last_indexed	2023-09-18T21:06:00Z
_version_	1777410936804474880

Investigation on the difference expression of type IV collagen α1(IV)- α6(IV) chain mrna in normal fibroblast and in skin cell malignant melanoma

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