Selective isolation and antagonistic activity of actinomycetes from mangrove forest of Pahang, Malaysia

This study aimed to isolate and evaluate the antagonistic potential of actinomycetes from mangrove forest of Pahang, Malaysia. Sediment samples from seven different sites were pretreated using wet heat and dry heat methods followed by dilution plating on eight isolation media. In total, 4850 isola...

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Main Authors:	Zainal Abidin, Zaima Azira, Abdul Malek, Nurfathiah, Zainuddin, Zarina, Khan Chowdhury, Ahmed Jalal
Format:	Article
Language:	English English English
Published:	Taylor & Francis 2016
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/46403/ http://irep.iium.edu.my/46403/ http://irep.iium.edu.my/46403/ http://irep.iium.edu.my/46403/1/46403_Selective%20isolation.pdf http://irep.iium.edu.my/46403/2/46403_Selective%20isolation_WOS.pdf http://irep.iium.edu.my/46403/3/46403_Selective%20isolation_SCOPUS.pdf

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recordtype	eprints
spelling	iium-464032017-02-08T02:00:20Z http://irep.iium.edu.my/46403/ Selective isolation and antagonistic activity of actinomycetes from mangrove forest of Pahang, Malaysia Zainal Abidin, Zaima Azira Abdul Malek, Nurfathiah Zainuddin, Zarina Khan Chowdhury, Ahmed Jalal QR Microbiology This study aimed to isolate and evaluate the antagonistic potential of actinomycetes from mangrove forest of Pahang, Malaysia. Sediment samples from seven different sites were pretreated using wet heat and dry heat methods followed by dilution plating on eight isolation media. In total, 4850 isolates were successfully recovered, with inorganic salt–starch agar displaying the highest percentage of recovery (31.7%), followed by actinomycete isolation agar (24.1%). The wet heat pretreatment was found to be more effective in the enumeration of actinomycetes, since 81.4% of total isolates were yielded using this technique, whereas dry heat treatment was better in the enumeration of spore-forming actinomycetes. After both pretreatments, Streptomyces-like and Micromonospora-like isolates were the most predominant. The antagonistic activities of the representative isolates were evaluated using the cross-streak method. In total, 78 isolates were active against at least one of the test organisms. Among them, 28.2% exhibited antibacterial activity, 23.1% exhibited antifungal activity and 48.7% displayed both. Nine isolates demonstrated broad antagonism by inhibiting the growth of all test organisms. The presence of a relatively large number of bioactive isolates suggests that Pahang mangrove forest is a potential source of actinomycetes with biosynthetic capabilities Taylor & Francis 2016 Article PeerReviewed application/pdf en http://irep.iium.edu.my/46403/1/46403_Selective%20isolation.pdf application/pdf en http://irep.iium.edu.my/46403/2/46403_Selective%20isolation_WOS.pdf application/pdf en http://irep.iium.edu.my/46403/3/46403_Selective%20isolation_SCOPUS.pdf Zainal Abidin, Zaima Azira and Abdul Malek, Nurfathiah and Zainuddin, Zarina and Khan Chowdhury, Ahmed Jalal (2016) Selective isolation and antagonistic activity of actinomycetes from mangrove forest of Pahang, Malaysia. Frontiers in Life Science, Vol. 9 (1). pp. 24-31. ISSN 2155-3769 E-ISSN 2155-3777 http://www.tandfonline.com/loi/tfls20#.VoC2YxV97IU 10.1080/21553769.2015.1051244
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	QR Microbiology
spellingShingle	QR Microbiology Zainal Abidin, Zaima Azira Abdul Malek, Nurfathiah Zainuddin, Zarina Khan Chowdhury, Ahmed Jalal Selective isolation and antagonistic activity of actinomycetes from mangrove forest of Pahang, Malaysia
description	This study aimed to isolate and evaluate the antagonistic potential of actinomycetes from mangrove forest of Pahang, Malaysia. Sediment samples from seven different sites were pretreated using wet heat and dry heat methods followed by dilution plating on eight isolation media. In total, 4850 isolates were successfully recovered, with inorganic salt–starch agar displaying the highest percentage of recovery (31.7%), followed by actinomycete isolation agar (24.1%). The wet heat pretreatment was found to be more effective in the enumeration of actinomycetes, since 81.4% of total isolates were yielded using this technique, whereas dry heat treatment was better in the enumeration of spore-forming actinomycetes. After both pretreatments, Streptomyces-like and Micromonospora-like isolates were the most predominant. The antagonistic activities of the representative isolates were evaluated using the cross-streak method. In total, 78 isolates were active against at least one of the test organisms. Among them, 28.2% exhibited antibacterial activity, 23.1% exhibited antifungal activity and 48.7% displayed both. Nine isolates demonstrated broad antagonism by inhibiting the growth of all test organisms. The presence of a relatively large number of bioactive isolates suggests that Pahang mangrove forest is a potential source of actinomycetes with biosynthetic capabilities
format	Article
author	Zainal Abidin, Zaima Azira Abdul Malek, Nurfathiah Zainuddin, Zarina Khan Chowdhury, Ahmed Jalal
author_facet	Zainal Abidin, Zaima Azira Abdul Malek, Nurfathiah Zainuddin, Zarina Khan Chowdhury, Ahmed Jalal
author_sort	Zainal Abidin, Zaima Azira
title	Selective isolation and antagonistic activity of actinomycetes from mangrove forest of Pahang, Malaysia
title_short	Selective isolation and antagonistic activity of actinomycetes from mangrove forest of Pahang, Malaysia
title_full	Selective isolation and antagonistic activity of actinomycetes from mangrove forest of Pahang, Malaysia
title_fullStr	Selective isolation and antagonistic activity of actinomycetes from mangrove forest of Pahang, Malaysia
title_full_unstemmed	Selective isolation and antagonistic activity of actinomycetes from mangrove forest of Pahang, Malaysia
title_sort	selective isolation and antagonistic activity of actinomycetes from mangrove forest of pahang, malaysia
publisher	Taylor & Francis
publishDate	2016
url	http://irep.iium.edu.my/46403/ http://irep.iium.edu.my/46403/ http://irep.iium.edu.my/46403/ http://irep.iium.edu.my/46403/1/46403_Selective%20isolation.pdf http://irep.iium.edu.my/46403/2/46403_Selective%20isolation_WOS.pdf http://irep.iium.edu.my/46403/3/46403_Selective%20isolation_SCOPUS.pdf
first_indexed	2023-09-18T21:06:04Z
last_indexed	2023-09-18T21:06:04Z
_version_	1777410941172842496

Selective isolation and antagonistic activity of actinomycetes from mangrove forest of Pahang, Malaysia

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