Diversity and antimicrobial activity of mangrove soil actinomycetes isolated from Tanjung Lumpur, Kuantan

Actinomycetes are biotechnologically important for their unrivalled capacity to produce bioactive secondary metabolites. Although thousands of antibiotics have been discovered from actinomycetes, these represent only a small fraction of the entire reservoir. Thus, screening of actinomycetes from poo...

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Main Authors:	Abdul Malek, Nurfathiah, Zainuddin, Zarina, Khan Chowdhury, Ahmed Jalal, Zainal Abidin, Zaima Azira
Format:	Article
Language:	English
Published:	Universiti Teknologi Malaysia 2015
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/46436/ http://irep.iium.edu.my/46436/ http://irep.iium.edu.my/46436/1/jurnal_teknologi__actino.pdf

id	iium-46436
recordtype	eprints
spelling	iium-464362016-05-12T06:12:02Z http://irep.iium.edu.my/46436/ Diversity and antimicrobial activity of mangrove soil actinomycetes isolated from Tanjung Lumpur, Kuantan Abdul Malek, Nurfathiah Zainuddin, Zarina Khan Chowdhury, Ahmed Jalal Zainal Abidin, Zaima Azira QR Microbiology Actinomycetes are biotechnologically important for their unrivalled capacity to produce bioactive secondary metabolites. Although thousands of antibiotics have been discovered from actinomycetes, these represent only a small fraction of the entire reservoir. Thus, screening of actinomycetes from poorly studied environment is a valuable attempt. In the present study, an effort was made to isolate, identify and evaluate the antimicrobial activities of actinomycetes from Tanjung Lumpur Mangrove Forest of Pahang, Malaysia; an underexplored ecosystem. Out of 1366 actinomycetes that were successfully enumerated, a total of 40 representative isolates were selected for further evaluation. The antimicrobial activity of the representative isolates were first assessed using the cross streak method against Bacillus subtilis, Candida albicans, Escherichia coli, Serratia marcescens and Staphylococcus aureus. Twenty five isolates displayed antagonistic potential against at least one of the test organisms. Of this, 40% exhibited antibacterial activity, 24% exhibited antifungal activity and 36% displayed both. The cell–free supernatant of the active isolates were then further evaluated using the agar well diffusion method, in which only 4 isolates displayed inhibitory activity. A total of 13 representative isolates were identified and characterized using 16S rRNA gene partial sequencing. They were further classified into 7 genera namely Streptomyces, Micromonospora, Rhodococcus, Gordonia, Pseudonocardia, Mycobacterium and Actinophytocola. These findings suggested that mangrove of Tanjung Lumpur is a rich source of actinomycetes for the discovery of bioactive secondary metabolites. Keywords: Diversity, mangrove, actinomycetes, Tanjung Lumpur, antimicrobial activity Universiti Teknologi Malaysia 2015-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/46436/1/jurnal_teknologi__actino.pdf Abdul Malek, Nurfathiah and Zainuddin, Zarina and Khan Chowdhury, Ahmed Jalal and Zainal Abidin, Zaima Azira (2015) Diversity and antimicrobial activity of mangrove soil actinomycetes isolated from Tanjung Lumpur, Kuantan. Jurnal Teknologi, 77 (25). pp. 37-43. ISSN 0127–9696 E-ISSN 2180–3722 http://www.jurnalteknologi.utm.my/index.php/jurnalteknologi
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Abdul Malek, Nurfathiah Zainuddin, Zarina Khan Chowdhury, Ahmed Jalal Zainal Abidin, Zaima Azira Diversity and antimicrobial activity of mangrove soil actinomycetes isolated from Tanjung Lumpur, Kuantan
description	Actinomycetes are biotechnologically important for their unrivalled capacity to produce bioactive secondary metabolites. Although thousands of antibiotics have been discovered from actinomycetes, these represent only a small fraction of the entire reservoir. Thus, screening of actinomycetes from poorly studied environment is a valuable attempt. In the present study, an effort was made to isolate, identify and evaluate the antimicrobial activities of actinomycetes from Tanjung Lumpur Mangrove Forest of Pahang, Malaysia; an underexplored ecosystem. Out of 1366 actinomycetes that were successfully enumerated, a total of 40 representative isolates were selected for further evaluation. The antimicrobial activity of the representative isolates were first assessed using the cross streak method against Bacillus subtilis, Candida albicans, Escherichia coli, Serratia marcescens and Staphylococcus aureus. Twenty five isolates displayed antagonistic potential against at least one of the test organisms. Of this, 40% exhibited antibacterial activity, 24% exhibited antifungal activity and 36% displayed both. The cell–free supernatant of the active isolates were then further evaluated using the agar well diffusion method, in which only 4 isolates displayed inhibitory activity. A total of 13 representative isolates were identified and characterized using 16S rRNA gene partial sequencing. They were further classified into 7 genera namely Streptomyces, Micromonospora, Rhodococcus, Gordonia, Pseudonocardia, Mycobacterium and Actinophytocola. These findings suggested that mangrove of Tanjung Lumpur is a rich source of actinomycetes for the discovery of bioactive secondary metabolites. Keywords: Diversity, mangrove, actinomycetes, Tanjung Lumpur, antimicrobial activity
format	Article
author	Abdul Malek, Nurfathiah Zainuddin, Zarina Khan Chowdhury, Ahmed Jalal Zainal Abidin, Zaima Azira
author_facet	Abdul Malek, Nurfathiah Zainuddin, Zarina Khan Chowdhury, Ahmed Jalal Zainal Abidin, Zaima Azira
author_sort	Abdul Malek, Nurfathiah
title	Diversity and antimicrobial activity of mangrove soil actinomycetes isolated from Tanjung Lumpur, Kuantan
title_short	Diversity and antimicrobial activity of mangrove soil actinomycetes isolated from Tanjung Lumpur, Kuantan
title_full	Diversity and antimicrobial activity of mangrove soil actinomycetes isolated from Tanjung Lumpur, Kuantan
title_fullStr	Diversity and antimicrobial activity of mangrove soil actinomycetes isolated from Tanjung Lumpur, Kuantan
title_full_unstemmed	Diversity and antimicrobial activity of mangrove soil actinomycetes isolated from Tanjung Lumpur, Kuantan
title_sort	diversity and antimicrobial activity of mangrove soil actinomycetes isolated from tanjung lumpur, kuantan
publisher	Universiti Teknologi Malaysia
publishDate	2015
url	http://irep.iium.edu.my/46436/ http://irep.iium.edu.my/46436/ http://irep.iium.edu.my/46436/1/jurnal_teknologi__actino.pdf
first_indexed	2023-09-18T21:06:07Z
last_indexed	2023-09-18T21:06:07Z
_version_	1777410944521994240

Diversity and antimicrobial activity of mangrove soil actinomycetes isolated from Tanjung Lumpur, Kuantan

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