Protein profiling of different plant tissues from herb Phyllanthus Niruri

Herb Phyllanthus niruri (P. niruri) is known to have various pharmacological functions including anticancer, antibacterial, antioxidant, anti-hypertensive and also anti-diabetic properties. In this research, the proteomic part of P. niruri was studied to determine the bioactive peptides that respon...

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Main Authors:	Ainul Mardhiah, Mohd Nail, Noor Hasniza, Md Zin
Format:	Article
Language:	English
Published:	Universiti Teknologi Malaysia 2015
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/46536/ http://irep.iium.edu.my/46536/1/Jurnal_Teknologi_Phyllanthus_niruri.pdf

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recordtype	eprints
spelling	iium-465362016-05-12T07:04:05Z http://irep.iium.edu.my/46536/ Protein profiling of different plant tissues from herb Phyllanthus Niruri Ainul Mardhiah, Mohd Nail Noor Hasniza, Md Zin Q Science (General) Herb Phyllanthus niruri (P. niruri) is known to have various pharmacological functions including anticancer, antibacterial, antioxidant, anti-hypertensive and also anti-diabetic properties. In this research, the proteomic part of P. niruri was studied to determine the bioactive peptides that responsible for specific characteristics. Total soluble proteins from different plant parts of freshly collected P. niruri were extracted using TCA/acetone method and then quantified using Bradford assay. Fruits part was found to have a significantly higher amount of proteins (4.91µg/µl + 0.21) compared to leaves (4.18µg/µl + 0.15). To determine the quality of proteins in the crude extract, SDS-Page was carried out which separates proteins in the basis of molecular weight. Proteins extracted from leaves were widely distributed between the range of 3.5 kDa to 160 kDA. Meanwhile, proteins in fruits mainly distributed within the range of 15 kDa to 80 kDa. The most highly expressed protein band was found in fruit, located in between 30 to 40 kDa. The protein extracts were then further analyzed based on the molecular weight and isoelectric points using two-dimensional gel electrophoresis (2D-GE) approach. Based on the profile pattern obtained from 2D-GE analysis, protein extract from fruits seems to express more protein spots compared to protein extract from leaves. Protein spots from fruit are seen to be intensely resolved within pH 4 to 10 at molecular weight between 10 kDa to 80 kDa. On the other hand, protein spots from leaves were moderately resolved at pH 4 to 10 at molecular weight within 10 kDa to 50 kDa. Universiti Teknologi Malaysia 2015-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/46536/1/Jurnal_Teknologi_Phyllanthus_niruri.pdf Ainul Mardhiah, Mohd Nail and Noor Hasniza, Md Zin (2015) Protein profiling of different plant tissues from herb Phyllanthus Niruri. Jurnal Teknologi, 77 (24). pp. 95-99. ISSN 2180-3722
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Ainul Mardhiah, Mohd Nail Noor Hasniza, Md Zin Protein profiling of different plant tissues from herb Phyllanthus Niruri
description	Herb Phyllanthus niruri (P. niruri) is known to have various pharmacological functions including anticancer, antibacterial, antioxidant, anti-hypertensive and also anti-diabetic properties. In this research, the proteomic part of P. niruri was studied to determine the bioactive peptides that responsible for specific characteristics. Total soluble proteins from different plant parts of freshly collected P. niruri were extracted using TCA/acetone method and then quantified using Bradford assay. Fruits part was found to have a significantly higher amount of proteins (4.91µg/µl + 0.21) compared to leaves (4.18µg/µl + 0.15). To determine the quality of proteins in the crude extract, SDS-Page was carried out which separates proteins in the basis of molecular weight. Proteins extracted from leaves were widely distributed between the range of 3.5 kDa to 160 kDA. Meanwhile, proteins in fruits mainly distributed within the range of 15 kDa to 80 kDa. The most highly expressed protein band was found in fruit, located in between 30 to 40 kDa. The protein extracts were then further analyzed based on the molecular weight and isoelectric points using two-dimensional gel electrophoresis (2D-GE) approach. Based on the profile pattern obtained from 2D-GE analysis, protein extract from fruits seems to express more protein spots compared to protein extract from leaves. Protein spots from fruit are seen to be intensely resolved within pH 4 to 10 at molecular weight between 10 kDa to 80 kDa. On the other hand, protein spots from leaves were moderately resolved at pH 4 to 10 at molecular weight within 10 kDa to 50 kDa.
format	Article
author	Ainul Mardhiah, Mohd Nail Noor Hasniza, Md Zin
author_facet	Ainul Mardhiah, Mohd Nail Noor Hasniza, Md Zin
author_sort	Ainul Mardhiah, Mohd Nail
title	Protein profiling of different plant tissues from herb Phyllanthus Niruri
title_short	Protein profiling of different plant tissues from herb Phyllanthus Niruri
title_full	Protein profiling of different plant tissues from herb Phyllanthus Niruri
title_fullStr	Protein profiling of different plant tissues from herb Phyllanthus Niruri
title_full_unstemmed	Protein profiling of different plant tissues from herb Phyllanthus Niruri
title_sort	protein profiling of different plant tissues from herb phyllanthus niruri
publisher	Universiti Teknologi Malaysia
publishDate	2015
url	http://irep.iium.edu.my/46536/ http://irep.iium.edu.my/46536/1/Jurnal_Teknologi_Phyllanthus_niruri.pdf
first_indexed	2023-09-18T21:06:15Z
last_indexed	2023-09-18T21:06:15Z
_version_	1777410952981905408

Protein profiling of different plant tissues from herb Phyllanthus Niruri

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